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Title Thyroid hormone regulation of epidermal growth factor receptor levels in mouse mammary glands
Creator/Author Vonderhaar, B.K. ; Tang, E. ; Lyster, R.R. ; Nascimento, M.C.
Publication Date1986 Aug 01
OSTI IdentifierOSTI ID: 5013379
Other Number(s)CODEN: ENDOA
Resource TypeJournal Article
Resource RelationEndocrinology ; Vol/Issue: 2
Research OrgNational Cancer Institute, Bethesda, MD
Subject551001 -- Physiological Systems-- Tracer Techniques; THYROID HORMONES-- BIOLOGICAL FUNCTIONS; CARCINOMAS;CHEMICAL BONDS;EPIDERMIS;GROWTH;HYPOTHYROIDISM;IODINE 125;LABELLED COMPOUNDS;LIVER;MAMMARY GLANDS;MICE;RECEPTORS;THYROXINE;TRACER TECHNIQUES
Related SubjectAMINO ACIDS;ANIMAL TISSUES;ANIMALS;BETA DECAY RADIOISOTOPES;BODY;CARBOXYLIC ACIDS;DAYS LIVING RADIOISOTOPES;DIGESTIVE SYSTEM;DISEASES;ELECTRON CAPTURE RADIOISOTOPES;ENDOCRINE DISEASES;EPITHELIUM;FUNCTIONS;GLANDS;HORMONES;INTERMEDIATE MASS NUCLEI;IODINE ISOTOPES;ISOTOPE APPLICATIONS;ISOTOPES;MAMMALS;MEMBRANE PROTEINS;NEOPLASMS;NUCLEI;ODD-EVEN NUCLEI;ORGANIC ACIDS;ORGANIC COMPOUNDS;ORGANIC HALOGEN COMPOUNDS;ORGANIC IODINE COMPOUNDS;ORGANS;PEPTIDE HORMONES;PROTEINS;RADIOISOTOPES;RODENTS;SKIN;THYROID HORMONES;TISSUES;VERTEBRATES
Description/Abstract The specific binding of iodinated epidermal growth factor ((/sup 125/I)iodo-EGF) to membranes prepared from the mammary glands and spontaneous breast tumors of euthyroid and hypothyroid mice was measured in order to determine whether thyroid hormones regulate the EGF receptor levels in vivo.^Membranes from hypothyroid mammary glands of mice at various developmental ages bound 50-65% less EGF than those of age-matched euthyroid controls.^Treatment of hypothyroid mice with L-T4 before killing restored binding to the euthyroid control level.^Spontaneous breast tumors arising in hypothyroid mice also bound 30-40% less EGF than tumors from euthyroid animals even after in vitro desaturation of the membranes of endogenous growth factors with 3 M MgCl2 treatment.^The decrease in binding in hypothyroid membranes was due to a decrease in the number of binding sites, not to a change in affinity of the growth factor for its receptor, as determined by Scatchard analysis of the binding data.^Both euthyroid and hypothyroid membranes bound EGF primarily to a single class of high affinity sites (dissociation constant (Kd) = 0.7-1.8 nM).^Euthyroid membranes bound 28.4 +/- (SE) 0.6 fmol/mg protein, whereas hypothyroid membranes bound 15.5 +/- 1.0 fmol/mg protein.^These data indicate that EGF receptor levels in normal mammary glands and spontaneous breast tumors in mice are subject to regulation by thyroid status.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 580-585
System Entry Date2001 May 13

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