Wang RY, Hayes M, Kotani H, Newton P, Shih J, Lo SC; American Society for Microbiology. General Meeting.
Abstr Gen Meet Am Soc Microbiol. 1992; 92: 159 (abstract no. G-2).
ARP, Armed Forces Inst. of Pathol., Wash. D.C.
An unusual organism (GTU-54), isolated from urine of an HIV+ male homosexual, has remarkable pathobiological properties of adhesion, hemadsorption and cytadsorption known to be associated with in vivo virulence. Biochemical, ultrastructural, antigenic and DNA studies revealed it to be a previously unknown mycoplasma that penetrates into the cytoplasm of infected mammalian cells through its unique tip-like structure. We have tentatively called the newly discovered mycoplasma M. penetrans. Subsequently, five additional isolates were cultured from the urines of HIV+ AIDS patients. In this study, we present antigenic and DNA comparison among these new clinical isolates. Protein analysis using Western blots and hyperimmune antiserum against GTU-54 show all the isolates have a similar protein profile and share antigenic determinants. DNA analyses using radiolabelled genomic DNA as well as molecularly cloned DNA from GTU-54 as probes also reveal a close homology among all six mycoplasmas, each has its distinct restriction pattern. Thus, in addition to sharing the unique biological and structural properties, all isolates of M. penetrans have extensive antigenic similarity and DNA homology.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Blotting, Western
- HIV Infections
- HIV Seropositivity
- Humans
- Male
- Mycoplasma
- Mycoplasma Infections
- Mycoplasma penetrans
- Virulence
Other ID:
UI: 102202442
From Meeting Abstracts