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Library:5719 (dbEST ID)
CT0484
Library Description
| Organism: | Homo sapiens |
| Developmental stage: | Adult |
| Organ: | colon |
| Vector: | puc18 |
| Vector type: | Plasmid |
| Cloning site 1: | SmaI |
| Cloning site 2: | SmaI |
A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.
Source: Simpson A.J.G., Ludwig Institute for Cancer Research
Gene Content Analysis
24 ESTs from this library were grouped into 20 UniGene entries (putative genes) [UniGene build #217, 04-Dec-2008]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).
| ESTs | TPM | UniGene Entry | ||
|---|---|---|---|---|
| 3 | 125000 |  |
Hs.199561 | Ran binding protein. |
| 2 | 83333 | |
Hs.632272 | Atrophin-1 interacting protein 4 (AIP4). |
| 2 | 83333 | |
Hs.257900 | Leucine zipper protein 1, mRNA (cDNA clone IMAGE:3922035). |
| 1 | 41667 | |
Hs.9825 | Family with sequence similarity 96, member B, mRNA (cDNA clone MGC:12554 IMAGE:3632169). |
| 1 | 41667 | |
Hs.690422 | Transcribed locus. |
| 1 | 41667 | |
Hs.596048 | Transcribed locus. |
| 1 | 41667 | |
Hs.532399 | Zinc finger CCCH-type containing 11A, mRNA (cDNA clone IMAGE:5262797). |
| 1 | 41667 | |
Hs.527653 | Decay-acceleration factor. |
| 1 | 41667 | |
Hs.523446 | CDNA FLJ57066 complete cds, highly similar to Collagen alpha-1(XI) chain precursor. |
| 1 | 41667 | |
Hs.522500 | KIAA0310 protein. |
| 1 | 41667 | |
Hs.513522 | TLS/CHOP=hybrid gene {translocation breakpoint} [human, myxoid liposarcomas cells, mRNA Mutant, 1682 nt]. |
| 1 | 41667 | |
Hs.513083 | Ribosomal protein L9, mRNA (cDNA clone MGC:29903 IMAGE:4996986). |
| 1 | 41667 | |
Hs.497391 | (xs160) mRNA, 389bp. |
| 1 | 41667 | |
Hs.419640 | RNA-binding protein regulatory subunit. |
| 1 | 41667 | |
Hs.413812 | MRNA, clone: PO2ST9. |
| 1 | 41667 | |
Hs.372003 | Clone 24815 unknown. |
| 1 | 41667 | |
Hs.195155 | Solute carrier family 38, member 5, mRNA (cDNA clone IMAGE:4649053). |
| 1 | 41667 | |
Hs.172028 | Transcribed locus. |
| 1 | 41667 | |
Hs.125898 | Adenyl cyclase mRNA. |
| 1 | 41667 | |
Hs.124126 | Actin related protein 2/3 complex, subunit 1A, 41kDa, mRNA (cDNA clone MGC:48820 IMAGE:6018804). |
Source: NCBI UniGene
EST Sequences
55 EST sequences from this library have been submitted to the public sequence database as of 04-Dec-2008.
Source: NCBI GenBank
Click the button to save sequences to a file (FASTA format)
Keywords
- intestine
- colorectal tumor
- adult
Source: NCBI Clone Registry
References
Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).