Bibliographic Citation
| Document | For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field. For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability. |
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| Title | Metabolic activation of carcinogenic aromatic amines by fish exposed to environmental pollutants |
| Creator/Author | Rodriguez-Ariza, A. ; Diaz-Mendez, F.M. ; Navas, J.I. ; Pueyo, C. ; Lopex-Barea, J. [Universidad de Cordoba, Huelva (Spain)] |
| Publication Date | 1995 Jul 01 |
| OSTI Identifier | OSTI ID: 70292 |
| Other Number(s) | EMMUEG; ISSN 0893-6692 |
| Resource Type | Journal Article |
| Resource Relation | Environmental and Molecular Mutagenesis ; VOL. 25 ; ISSUE: 1 ; PBD: 1995 |
| Subject | 56 BIOLOGY AND MEDICINE, APPLIED STUDIES ;54 ENVIRONMENTAL SCIENCES ; AMINES; METABOLIC ACTIVATION; PESTICIDES; BIOLOGICAL EFFECTS; FISHES; BIOCHEMISTRY; COPPER; WATER POLLUTION; BIOLOGICAL INDICATORS |
| Description/Abstract | Activation of arylamines to mutagenic metabolites by hepatic S9 fractions has been evaluated as a biomaker of fish exposure to pollutants, using gilthead seabream (Sparus aurata), a valuable fish species from the Spanish South Atlantic littoral, as model organism. To obtain maximum sensitivity to the mutagenic action of aromatic amines, a strain of Salmonella typhimurium overproducing O-acetyltransferase was used. Fish were treated with Aroclor 1254, pesticides (malathion and dieldrin), or copper (II), and compared to Aroclor 1254-treated rats. The promutagen activation capabilities of the S9 fractions were further characterized by studying the effect of two monoxygenase inhibitors,{alpha}-naphthoflavone, a well known inhibitor of aromatic hydrocarbon-inducible forms of cytochrome P450, and methimazole, a substrate for the flavin monoxygenase (FMO) system. This study shows that 2-aminoanthracene (2-AA) and 2-acetylaminofluorene (AAF) activation by gilthead liver is enhanced by treatment of fish with different xenobiotics. The catalyst responsible for this enhanced activation appears to be different for each promutagen and, at least for 2-AA, dependent on the type of xenobiotic. The data presented indicate further that treatment of gilthead with some compounds, such as malathion and dieldrin, enhances the activation of aromatic amines in liver, without inducing ethoxyresorufin-O-deethylase activity. The use of acetyltransferase-overproducing bacteria appears to be a useful tool in the study of arylamine activation by fish liver, where biotransformation capability is lower than in mammals. |
| Country of Publication | United States |
| Language | English |
| Format | pp. 50-57 ; PL: |
| System Entry Date | 2001 May 03 |
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