OXYTOCIN AND NEUROHYPOPHYSEAL PEPTIDES: SPECTRAL ASSIGNMENT AND CONFORMATIONAL ANALYSIS BY 220 MHz NUCLEAR MAGNETIC RESONANCE

Proc Natl Acad Sci U S A. 1969 December; 64(4): 1269–1275.

PMCID: PMC223279

OXYTOCIN AND NEUROHYPOPHYSEAL PEPTIDES: SPECTRAL ASSIGNMENT AND CONFORMATIONAL ANALYSIS BY 220 MHz NUCLEAR MAGNETIC RESONANCE^*^†

LeRoy F. Johnson, I. L. Schwartz, and Roderich Walter^‡

VARIAN ASSOCIATES, ANALYTICAL INSTRUMENT DIVISION, PALO ALTO, CALIF.

DEPARTMENT OF PHYSIOLOGY, MOUNT SINAI MEDICAL AND GRADUATE SCHOOLS OF THE CITY UNIVERSITY OF NEW YORK

MEDICAL RESEARCH CENTER, BROOKHAVEN NATIONAL LABORATORY, UPTON, NEW YORK

^‡ To whom reprint requests should be addressed.

^* This work was supported, in part, by U.S. Public Health Service grant AM 10080 of the National Institute of Arthritis and Metabolic Diseases and by the U.S. Atomic Energy Commission.

^† Neurohypophyseal hormones are denoted in accordance with the IUPAC-IUB Tentative Rules (Biochemistry, 6, 362 (1967)) and standard abbreviations are used for amino acid residues (Biochemistry, 5, 2485 (1966)). The amino acids (except glycine) are of the L-configuration.

This article has been cited by other articles in PMC.

Abstract

Magnetic resonance peaks have been assigned to individual protons of the constituent amino acids in the neurohypophyseal hormone, oxytocin, and in related peptides. The assignments were made possible by operation at 220 MHz with the use of variable temperature studies, proton homonuclear spin-decoupling, and comparison of spectra of oxytocin analogs. Some of the observed chemical shifts, and NH-CH^α coupling constants were studied in relation to the conformation of the hormone.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (803K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

Gordon, W; Havran, RT; Schwartz, IL; Walter, R. Conformational differences in neurohypophyseal peptides: a countercurrent distribution and partition chromatography study. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1353–1355. [PubMed]
Urry, DW; Quadrifoglio, F; Walter, R; Schwartz, IL. Conformational studies on neurohypophyseal hormones: the disulfide bridge of oxytocin. Proc Natl Acad Sci U S A. 1968 Jul;60(3):967–974. [PubMed]
Ferrier, BM; Jarvis, D; Du Vigneaud, V. Deamino-oxytocin. Its isolation by partition chromatography on Sephadex and crystallization from water, and its biological activities. J Biol Chem. 1965 Nov;240(11):4264–4266. [PubMed]
MANDEL, M. PROTON MAGNETIC RESONANCE SPECTRA OF SOME PROTEINS. I. RIBONUCLEASE, OXIDIZED RIBONUCLEASE, LYSOZYME, AND CYTOCHROME C. J Biol Chem. 1965 Apr;240:1586–1592. [PubMed]
Beecham, AF; Ham, NS. Proton magnetic resonance spectra of some peptides of L-leucine and glycine. Tetrahedron. 1968 Mar;24(6):2773–2781. [PubMed]
Stern, A; Gibbons, WA; Craig, LC. A conformational analysis of gramicidin S-A by nuclear magnetic resonance. Proc Natl Acad Sci U S A. 1968 Oct;61(2):734–741. [PubMed]
McDonald, CC; Phillips, WD. Proton magnetic resonance spectra of proteins in random-coil configurations. J Am Chem Soc. 1969 Mar 12;91(6):1513–1521. [PubMed]