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Research Program My investigative program falls within the general theme of Environmentally Acceptable Endpoints, which is divided into three research areas: 1)
Molecular mechanisms of microbial transformation of metal oxyanions
and aromatic organic compounds; 2)
Development and use of synchrotron radiation-based spectromicroscopy
and other molecular probes for detecting and imaging cellular responses
to stimuli in living systems; and 3) Development of in vivo and in vitro methods for characterizing oral absorption and biokinetics of environmental contaminants. Professional Experience
Academic Degrees Ph.D. University of California at Berkeley, College of Engineering, 1986 Major: Environmental Chemistry and Engineering Minor: Chemistry and Chemical Engineering Awards 1998 Lawrence Berkeley National Laboratory Outstanding Scientific Performance Award Patents Holman, H.-Y. N. Spectroscopic evaluation of atherosclerotic plaques, IB-1867P, 2003. Holman, H.-Y. N. Gastrointestinal mimetic device, U.S. Patent No. 6,040,188, 2000. Holman, H.-Y. N. In situ infrared spectroscopy profiling of intracellular responses to external stimuli, DOE Patent Docket No.: S-91591, 1999. Publication Activities Tsibakhashvili NY. Mosulishvili LM. Kalabegishvili TL. Kirkesali EI. Frontasyeva MV. Pomyakushina EV. Pavlov SS. Holman HYN. ENAA studies of chromium uptake by Arthrobacter oxydans. Journal of Radioanalytical & Nuclear Chemistry. 259(3):527-531, 2004. Asatiani, N., Sapojnikova, N., Abuladze, M., Kartvelishvili, T.L., Kulikova, N., Kiziria, E., Namchevadze, E., and H.-Y. N. Holman. “Effects of Cr(VI) long-term and low-dose action on mammalian antioxidant enzymes: an in vitro study”. J. Inorganic Biochemistry, 98, 490-496, 2004. Tsibakhashvili, N.Y., Mosulishvili, N.A., Kalabegishvili, T.L., Kirkesali, T.I., Frontasyeva, M.V., Pomyakushina, E.V., Pavlov, S.S., and H.-Y. N. Holman. “Epithermal neutron activation analysis (ENAA) studies of chromium uptake by Arthrobacter oxydans”. Journal of Radioanalytical and Nuclear Chemistry, 259 (1/2), 2004. Holman, H.-Y. N., Martin, M.C. and W.R. McKinney. "Tracking chemical changes in a live cell: Biomedical Applications of SR-FTIR Spectromicroscopy", in Special issue: First International Conference onBiomedical Spectroscopy: From Molecules to men”, Spectroscopy - An International Journal, 17(2-3), 139-160. 2003. Holman, H.-Y. N., Martin, M.C. and W. R. McKinney "Synchrotron-Based FTIR Spectromicroscopy: Cytotoxicity Considerations" J. Biological Physics 29, 275-286, 2003. Kalabegishvili, T.L, Tsibakhashvili, N.Y. and H.-Y. N. Holman. “Electron spin resonance study of chromium(V) formation and decomposition by basalt-inhabiting bacteria”. Environmental Science & Technology, 37, 4678-4684, 2003. Bakradze, N., Sokhadze, V., Abuladze, M., Asatiani, N., Sapojnikova, N., Kartvelishvili, T.L., Tsibakhashvili, N.Y., Namchevadze, E., Tabatadze, L., Lejava, L., and H.-Y.N. Holman. “A Calorimetric Characterization of Cr(VI)-reducing Arthrobacter oxydans at different phases of the cell growth cycle”. TheScientificWorldJournal, 3, 432-442, 2003. Holman, H.-Y. N., Bjornstad, K.A., McNamara, M.P., Martin, M.C., McKinney, W.R., and E.A. Blakely. “Synchrotron Infrared Spectromicroscopy as a Novel Bioanalytical Microprobe for Individual Living Cells: Cytotoxicity Considerations”. J. of Biomedical Optics, 7(3), 417-424, 2002. Holman, H.-Y. N., Nieman, K., Sorensen, D.L., Miller, C.D., Martin, M.C.; Borch, T., McKinney, W.R., and R.C. Sims. “Catalysis of PAH Biodegradation by Humic Acid Shown in Synchrotron Infrared Studies”. Environmental Science & Technology, 36(6): 1276-1280, 2002. Holman, H.-Y. N., Goth-Goldstein, R., David Aston, Mao, Y., and J. Kengsoontra “Evaluation of gastrointestinal solubilization of petroleum hydrocarbon residues in soil using an in vitro Physiologically-Based Model”. Environmental Science & Technology, 36(6):1281-1286, 2002. Holman, H.-Y. N., Bjornstad, K.A., McNamara, M.P., Martin, M.C., McKinney, W.R., and E.A. Blakely. “Synchrotron Infrared Spectromicroscopy as a Novel Bioanalytical Microprobe for Individual Living Cells: Cytotoxicity Considerations”. J. of Biomedical Optics, 7(3), 417-424, 2002. Tsibakhashvili, N.Y., Asatiani, N.V., Abuladze, M.K., Birkaya, B.G., Sapojnikova, N.A., Mosulishvili, L.M., and H.-Y. N. Holman. “Capillary Electrophoresis of Cr(VI) Reducer Arthrobacter oxydans”. Biomedical Chromatography, 16(5): 327-331, 2002. Bhupathiraju, V. K., Krauter, P., Holman, H-Y. N., Conrad, M. E., Daley, P. F., Templeton, A. S., Hunt, J. R., Hernandez, M., and L. Alvarez-Cohen. "Assessment of in-situ bioremediation at a refinery waste-contaminated site and an aviation gasoline contaminated site". Biodegradation, 13(2):79-90, 2002. Abuladze, M.K., Asatiani, N.V., Bakradze, N. G., Kartvelishvili, T.M., Holman, H.-Y. N., Kalabegishvili, T.L., Mosulishvili, L.M., Rcheulishvili, A.N., Sapojnikova, N.A., and N. Y. Tsibakhashvili. “Effect of Chromium Action on the Protein Composition of A. oxydans" Fresenius of Environmental Bulletin, 11(9A): 562-567, 2002. Tsibakhashvili N.Y., Mosulishvili, L.M., Kalabegishvili, T.L., Pataraya, D.T., Gurielidze, M.A., and G.S. Nadareishvili, and H.-Y. N. Holman. “Chromate-Resistant and –Reducing Microorganisms in Georgia Basalts: Their Distribution and Characterization”. Fresenius of Environmental Bulletin, 11(10), 352-361, 2002. Holman, H.-Y. N., Goth-Goldstein, R., Martin, M.C., Russell, M.L., and W.R. McKinney. "Low-dose responses to 2,3,7,8-tetrachlorodibenzo-p-dioxin in single living human cells measured by synchrotron infrared spectromicroscopy,” Environ. Sci. & Tech.,34 (12), 2513-2517, 2000. Holman, H.-Y. N., Martin, M.C., Blakely, E.A., Bjornstad, K., and W.R. McKinney. "Infrared spectroscopic characteristics of cell cycle and cell death probed by synchrotron-based FTIR spectromicroscopy,” Biopolymers: Biospectroscopy, 57[6], 329-335, 2000. Holman, H.-Y. N., Goth-Goldstein, R., Blakely, E.A., Bjornstad, K., Martin, M.C., and W.R. McKinney. "Individual Human Cell Responses to Low Doses of Chemicals Studied by Synchrotron Infrared Spectromicroscopy", inBiomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques, SPIE Vol. 3918, 57-63, 2000. Geller, J.T., Holman, H.-Y. N., Su, G., Conrad, M.E., Pruess, K., and J.C. Hunter-Cevera. “Flow dynamics and potential for biodegradation of organic contaminants in fractured rock vadose zones,” J. Contaminant Hydrology, 43(1), 63-90, 2000. Holman, H.-Y. N., Perry, D.L., Martin, M.C., Geraldine Lamble, W.R. McKinney, and J.C. Hunter-Cevera. “Real-time Characterization of Biogeochemical Reduction of Cr(VI) on Basalt Surfaces by SR-FTIR Imaging,” Geomicrobiology J., 16(4), 307-323. 1999. Holman, H.-Y. N., Tsang, Y.W., and W.R. Holman. Mineralization of sparsely water-soluble polycyclic aromatic hydrocarbons in a water table fluctuation zone. Environ. Sci. Technol., 33,1819-1824, 1999. Holman, H.-Y.N., Zhang, M., Goth-Goldstein R., Martin, M.C., Russell, M., McKinney W.R., Ferrari, M., and J.C. Hunter-Cevera. “Detecting exposure to environmental organic toxins in individual cells: towards development of a micro-fabricated device,” Micro-Nanofabricated Structures and Devices for Biomedical Environmental Applications II, SPIE, 3606,55-62, 1999. Zhang, M., Holman, H.-Y. N., Ferrari, M., and J.C. Hunter-Cevera. “Synchrotron infrared microspectroscopy for assessment of mutagenicity of metal implants,” Biomedical Materials - Drug Delivery, Implants and Tissue Engineering, MRS Symp. Series, 163-169,1999. Holman, H.-Y. N., Perry, D.L., and J.C. Hunter-Cevera. Surface-enhanced infrared absorption-reflectance (SEIRA) microspectroscopy – a chemical/biological probe for bacteria localization in geologic materials. J. Microbiol. Methods, 34/1, 59-71, 1998. Holman, H.-Y. N., Perry, D.L., Martin, M.C., and W.R. McKinney. Applications of synchrotron infrared microspectroscopy to the study of inorganic-organic interactions at the bacterial-mineral interface, Application of Synchrotron Radiation Techniques to Materials Sciences, MRS Symp. Series, 17-24, 1998. Holman, H.-Y. N., and I. Javandel. Evaluation of transient dissolution of slightly water-soluble compounds from a light nonaqueous phase liquid pool, Water Resources Research, 32, 915-923, 1996. Holman, H.-Y. N., and Y.W. Tsang. Effects of soil moisture on biodegradation of petroleum hydrocarbons. In: In situ aeration: air sparging, bioventing and related remediation processes, Battelle Press, Richland. 323-332, 1995. Javandel, I., Falta, R.W., and H.-Y.N. Holman. Recent developments in transport and fate of nonaqueous phase liquids in the subsurface environment, Iran. J. of Sci. and Technol., 14, 269, 1990.
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