R = Retrieve links to Related sequences (neighbors).
Use the 
icon (if present) to retrieve links to the Sequence Retrieval System (SRS).
Use the 
icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .
Use the 
icon (if present) to retrieve links to the Protein Data Bank database.
WARNING: Descriptions of 941 database sequences were not reported due to the
limiting value of parameter V = 50.
>gi|7484829|pir||T08398 calmodulin homolog F18B3.50 - Arabidopsis thaliana
>gi|4835228|emb|CAB42906.1| (AL049862) calmodulin-like protein
[Arabidopsis thaliana]
Length = 205
Frame 1 hits (HSPs): ___________________________________
__________________________________________________
Database sequence: | | | | | | 205
0 50 100 150 200
Plus Strand HSPs:
Score = 158 (55.6 bits), Expect = 1.1e-10, P = 1.1e-10
Identities = 38/85 (44%), Positives = 51/85 (60%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
E +V +FD DGDGKIS ELR+ G +G + + A++ I E+D+D DG L EDFV
Sbjct: 64 ELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVG 123
Query: 268 LM---EAAGEDEKLKD--LEEAFEM 327
LM + G+ E D L+ AFEM
Sbjct: 124 LMTRRDLYGDGEVDGDGELKTAFEM 148
Score = 76 (26.8 bits), Expect = 0.64, P = 0.47
Identities = 19/63 (30%), Positives = 32/63 (50%), Frame = +1
Query: 88 EFERVLKYFD-EDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFV 264
E + + F+ E G G I+P L+ L +G + E +I+ D DG+G L +F
Sbjct: 141 ELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYDIDGNGILDFHEFR 200
Query: 265 KLM 273
++M
Sbjct: 201 QMM 203
>gi|5391446|gb|AAC15474.2| (AF031471) pollen allergen [Juniperus oxycedrus]
Length = 165
Frame 1 hits (HSPs): _____________________________________________
__________________________________________________
Database sequence: | | | | | 165
0 50 100 150
Plus Strand HSPs:
Score = 157 (55.3 bits), Expect = 1.4e-10, P = 1.4e-10
Identities = 35/86 (40%), Positives = 54/86 (62%), Frame = +1
Query: 70 VMRMNT-EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFL 246
VM + E E V K FD +GDGKIS EL + L +G ++ + + ++EE D+DGDG++
Sbjct: 19 VMEQSVHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADADGDGYV 78
Query: 247 SLEDFVKLMEAAGEDEKLKDLEEAFEM 327
SL++FV L +KDL+ AF++
Sbjct: 79 SLQEFVDLNNKGAS---VKDLKNAFKV 102
Score = 109 (38.4 bits), Expect = 1.7e-05, P = 1.7e-05
Identities = 21/69 (30%), Positives = 39/69 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
+ + K FD D +G IS EL + L +G ++++ +I +D +GDG +S+E+F
Sbjct: 95 DLKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHNVDKNGDGLISVEEFQT 154
Query: 268 LMEAAGEDE 294
+M + D+
Sbjct: 155 MMTSEMTDK 163
>gi|2213610|gb|AAB61505.1| (AC000103) F21J9.4 [Arabidopsis thaliana]
Length = 186
Frame 1 hits (HSPs): ______________________________________
__________________________________________________
Database sequence: | | | | | 186
0 50 100 150
Plus Strand HSPs:
Score = 150 (52.8 bits), Expect = 7.6e-10, P = 7.6e-10
Identities = 32/81 (39%), Positives = 53/81 (65%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
E E V K FD +GDGKIS EL + +G E+ ++ EK I E+D GDG+++ E+FV+
Sbjct: 37 ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVE 96
Query: 268 L-MEAAGEDEKLKDLEEAFEM 327
L + +++ L++L++AF +
Sbjct: 97 LNTKGMDQNDVLENLKDAFSV 117
Score = 107 (37.7 bits), Expect = 3.3e-05, P = 3.3e-05
Identities = 28/81 (34%), Positives = 41/81 (50%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFERV---LKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
F+ + +K M N E + +D DG+G IS EL L +G E + K+I
Sbjct: 94 FVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGG 153
Query: 220 LDSDGDGFLSLEDFVKLMEAAG 285
+D DGDG + E+F K+M G
Sbjct: 154 VDKDGDGTIDFEEF-KIMMTMG 174
>gi|6143901|gb|AAF04447.1|AC010718_16 (AC010718) putative calmodulin
[Arabidopsis thaliana]
Length = 177
Frame 1 hits (HSPs): _______________________________________
__________________________________________________
Database sequence: | | | | | 177
0 50 100 150
Plus Strand HSPs:
Score = 149 (52.5 bits), Expect = 9.7e-10, P = 9.7e-10
Identities = 31/82 (37%), Positives = 48/82 (58%), Frame = +1
Query: 82 NTEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDF 261
N E E V Y D + DG+ISP EL+ +G +L ++A + D+DGDG L E+F
Sbjct: 41 NRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEF 100
Query: 262 VKLMEAAGEDEKLKDLEEAFEM 327
+L++ E+EK +L+ AF +
Sbjct: 101 SQLIKVDDEEEKKMELKGAFRL 122
Score = 75 (26.4 bits), Expect = 0.91, P = 0.60
Identities = 19/77 (24%), Positives = 39/77 (50%), Frame = +1
Query: 61 FSKVMRMNTEFERVL------KYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEEL 222
FS++++++ E E+ + + + +G+ I+P L+ L +G D +I
Sbjct: 100 FSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAF 159
Query: 223 DSDGDGFLSLEDFVKLM 273
D + DG LS ++F +M
Sbjct: 160 DLNADGVLSFDEFALMM 176
>gi|115506|sp|P04352|CALM_CHLRE CALMODULIN >gi|71688|pir||MCKM calmodulin -
Chlamydomonas reinhardtii >gi|167411|gb|AAA33083.1| (M20729)
calmodulin [Chlamydomonas reinhardtii]
Length = 163
Frame 1 hits (HSPs): _____________________________________________
Annotated Domains: ________________________ _____________________
__________________________________________________
Database sequence: | | | | | 163
0 50 100 150
__________________
Annotated Domains:
BLOCKS BL00018: EF-hand calcium-binding domain 132..144
DOMO DM00011: CALMODULINREPEAT 1..35
Entrez acetylation site 2
Entrez Calcium binding region: SITE 1. 24..35
Entrez Calcium binding region: SITE 2. 60..71
Entrez Calcium binding region: SITE 3. 97..108
Entrez Calcium binding region: SITE 4. 133..144
PFAM efhand: EF hand 14..42
PFAM efhand: EF hand 50..78
PFAM efhand: EF hand 87..115
PFAM efhand: EF hand 123..151
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 8..74
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 86..147
PROSITE EF_HAND: EF-hand calcium-binding domain. 133..145
PROSITE EF_HAND: EF-hand calcium-binding domain. 24..36
PROSITE EF_HAND: EF-hand calcium-binding domain. 60..72
PROSITE EF_HAND: EF-hand calcium-binding domain. 97..109
__________________
Plus Strand HSPs:
Score = 146 (51.4 bits), Expect = 2.0e-09, P = 2.0e-09
Identities = 31/88 (35%), Positives = 53/88 (60%), Frame = +1
Query: 49 FLRVFSKVMRMNT---EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ M+ E K FD+DG+G IS ELR+ + +G +L ++ +++I E
Sbjct: 72 FLMLMARKMKETDHEDELREAFKVFDKDGNGFISAAELRHVMTNLGEKLSEEEVDEMIRE 131
Query: 220 LDSDGDGFLSLEDFVKLMEAAGEDEKLK 303
D DGDG ++ E+FV++M + D+K K
Sbjct: 132 ADVDGDGQVNYEEFVRMMTSGATDDKDK 159
Score = 117 (41.2 bits), Expect = 2.4e-06, P = 2.4e-06
Identities = 26/81 (32%), Positives = 45/81 (55%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 15 EFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMISEVDADGNGTIDFPEFLM 74
Query: 268 LMEAAGEDEKLKD-LEEAFEM 327
LM ++ +D L EAF++
Sbjct: 75 LMARKMKETDHEDELREAFKV 95
Score = 69 (24.3 bits), Expect = 8.4, P = 1.0
Identities = 26/80 (32%), Positives = 40/80 (50%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E L ++ ++ D E + E D DG+GF+S
Sbjct: 51 ELQDMISEVDADGNGTIDFPEF---LMLMARKMKETDHEDELREAFKVFDKDGNGFISAA 107
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL + EE EM
Sbjct: 108 ELRHVMTNLGE--KLSE-EEVDEM 128
>gi|225024|prf||1206346A calmodulin [Chlamydomonas reinhardtii]
Length = 162
Frame 1 hits (HSPs): _____________________________________________
__________________________________________________
Database sequence: | | | | | 162
0 50 100 150
Plus Strand HSPs:
Score = 146 (51.4 bits), Expect = 2.0e-09, P = 2.0e-09
Identities = 31/88 (35%), Positives = 53/88 (60%), Frame = +1
Query: 49 FLRVFSKVMRMNT---EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ M+ E K FD+DG+G IS ELR+ + +G +L ++ +++I E
Sbjct: 71 FLMLMARKMKETDHEDELREAFKVFDKDGNGFISAAELRHVMTNLGEKLSEEEVDEMIRE 130
Query: 220 LDSDGDGFLSLEDFVKLMEAAGEDEKLK 303
D DGDG ++ E+FV++M + D+K K
Sbjct: 131 ADVDGDGQVNYEEFVRMMTSGATDDKDK 158
Score = 117 (41.2 bits), Expect = 2.4e-06, P = 2.4e-06
Identities = 26/81 (32%), Positives = 45/81 (55%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 14 EFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMISEVDADGNGTIDFPEFLM 73
Query: 268 LMEAAGEDEKLKD-LEEAFEM 327
LM ++ +D L EAF++
Sbjct: 74 LMARKMKETDHEDELREAFKV 94
Score = 69 (24.3 bits), Expect = 8.3, P = 1.0
Identities = 26/80 (32%), Positives = 40/80 (50%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E L ++ ++ D E + E D DG+GF+S
Sbjct: 50 ELQDMISEVDADGNGTIDFPEF---LMLMARKMKETDHEDELREAFKVFDKDGNGFISAA 106
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL + EE EM
Sbjct: 107 ELRHVMTNLGE--KLSE-EEVDEM 127
>gi|4678921|emb|CAB41312.1| (AL049711) putative calmodulin [Arabidopsis
thaliana] >gi|5825602|gb|AAD53315.1|AF178075_1 (AF178075)
calmodulin 9 [Arabidopsis thaliana]
Length = 151
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | || 151
0 50 100 150
Plus Strand HSPs:
Score = 144 (50.7 bits), Expect = 3.3e-09, P = 3.3e-09
Identities = 30/65 (46%), Positives = 41/65 (63%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
E V + FD DGDG IS EL + +G ++ A++AE ++ E D DGDGFLS +F K
Sbjct: 85 ELIEVFRVFDRDGDGLISQLELGEGMKDMGMKITAEEAEHMVREADLDGDGFLSFHEFSK 144
Query: 268 LMEAA 282
+M AA
Sbjct: 145 MMIAA 149
Score = 82 (28.9 bits), Expect = 0.014, P = 0.014
Identities = 21/81 (25%), Positives = 40/81 (49%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF D+D DG I+ +L + +G A+ ++++ ++D G+G ++ +DF+
Sbjct: 12 EFYEAFCLIDKDSDGFITKEKLTKVMKSMGKNPKAEQLQQMMSDVDIFGNGGITFDDFLY 71
Query: 268 LMEAAGEDEKLKD-LEEAFEM 327
+M E D L E F +
Sbjct: 72 IMAQNTSQESASDELIEVFRV 92
>gi|115528|sp|P27166|CALM_STYLE CALMODULIN >gi|102408|pir||JC1309 calmodulin -
Stylonychia lemnae >gi|161195|gb|AAA29966.1| (M76407) Calmodulin
[Stylonychia lemnae]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
Annotated Domains: _________________________ ________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
__________________
Annotated Domains:
BLOCKS BL00018: EF-hand calcium-binding domain 129..141
DOMO DM00011: CALMODULINREPEAT 1..32
Entrez methylation site: (BY SIMILARITY). 116
Entrez Calcium binding region: SITE 1. 21..32
Entrez Calcium binding region: SITE 2. 57..68
Entrez Calcium binding region: SITE 3. 94..105
Entrez Calcium binding region: SITE 4. 130..141
PFAM efhand: EF hand 11..39
PFAM efhand: EF hand 47..75
PFAM efhand: EF hand 84..112
PFAM efhand: EF hand 120..148
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 5..71
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 81..144
PROSITE EF_HAND: EF-hand calcium-binding domain. 130..142
PROSITE EF_HAND: EF-hand calcium-binding domain. 21..33
PROSITE EF_HAND: EF-hand calcium-binding domain. 57..69
PROSITE EF_HAND: EF-hand calcium-binding domain. 94..106
__________________
Plus Strand HSPs:
Score = 143 (50.3 bits), Expect = 4.2e-09, P = 4.2e-09
Identities = 32/80 (40%), Positives = 52/80 (65%), Frame = +1
Query: 49 FLRVFSKVMR-MNTEFERV--LKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ M+ +TE E V K FD DG+G IS ELR+ + +G +L ++ +++I E
Sbjct: 69 FLSLMARKMKDTDTEEELVEAFKVFDRDGNGLISAAELRHVMTNLGEKLTDEEVDEMIRE 128
Query: 220 LDSDGDGFLSLEDFVKLMEA 279
D DGDG ++ E+FV++M A
Sbjct: 129 ADVDGDGHINYEEFVRMMMA 148
Score = 120 (42.2 bits), Expect = 1.1e-06, P = 1.1e-06
Identities = 26/81 (32%), Positives = 46/81 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLS 71
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
LM +D + ++L EAF++
Sbjct: 72 LMARKMKDTDTEEELVEAFKV 92
Score = 71 (25.0 bits), Expect = 3.1, P = 0.95
Identities = 27/80 (33%), Positives = 41/80 (51%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEK-LIEEL---DSDGDGFLSLE 255
E + ++ D DG+G I E L ++ ++ D E+ L+E D DG+G +S
Sbjct: 48 ELQDMINEVDADGNGTIDFPEF---LSLMARKMKDTDTEEELVEAFKVFDRDGNGLISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 105 ELRHVMTNLGE--KLTD-EEVDEM 125
>gi|2267084|gb|AAB63506.1| (AF007889) calmodulin [Symbiodinium microadriaticum]
Length = 138
Frame 1 hits (HSPs): __________________________________________________
__________________________________________________
Database sequence: | | | | 138
0 50 100
Plus Strand HSPs:
Score = 142 (50.0 bits), Expect = 5.3e-09, P = 5.3e-09
Identities = 32/80 (40%), Positives = 52/80 (65%), Frame = +1
Query: 49 FLRVFSKVMR-MNTEFERV--LKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ M+ +TE E + K FD DG+G IS ELR+ + +G +L ++ +++I E
Sbjct: 58 FLSLMARKMKDTDTEEELIEAFKVFDRDGNGFISAAELRHVMTNLGEKLTDEEVDEMIRE 117
Query: 220 LDSDGDGFLSLEDFVKLMEA 279
D DGDG ++ E+FVK+M A
Sbjct: 118 ADVDGDGQINYEEFVKMMMA 137
Score = 119 (41.9 bits), Expect = 1.5e-06, P = 1.5e-06
Identities = 26/81 (32%), Positives = 46/81 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 1 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLS 60
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
LM +D + ++L EAF++
Sbjct: 61 LMARKMKDTDTEEELIEAFKV 81
Score = 78 (27.5 bits), Expect = 0.062, P = 0.060
Identities = 29/80 (36%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEK-LIEEL---DSDGDGFLSLE 255
E + ++ D DG+G I E L ++ ++ D E+ LIE D DG+GF+S
Sbjct: 37 ELQDMINEVDADGNGTIDFPEF---LSLMARKMKDTDTEEELIEAFKVFDRDGNGFISAA 93
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 94 ELRHVMTNLGE--KLTD-EEVDEM 114
>gi|115521|sp|P11120|CALM_PLECO CALMODULIN >gi|71675|pir||MCMRP calmodulin -
cornucopia mushroom
Length = 148
Frame 1 hits (HSPs): ______________________________________________
Annotated Domains: __________________________ _______________________
__________________________________________________
Database sequence: | | | | 148
0 50 100
__________________
Annotated Domains:
BLOCKS BL00018: EF-hand calcium-binding domain 129..141
DOMO DM00011: CALMODULINREPEAT 1..32
Entrez acetylation site 1
Entrez Calcium binding region: SITE 1. 20..31
Entrez Calcium binding region: SITE 2. 56..67
Entrez Calcium binding region: SITE 3. 93..104
Entrez Calcium binding region: SITE 4. 129..140
PFAM efhand: EF hand 11..39
PFAM efhand: EF hand 47..75
PFAM efhand: EF hand 84..112
PFAM efhand: EF hand 120..148
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 5..71
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 81..144
PROSITE EF_HAND: EF-hand calcium-binding domain. 129..141
PROSITE EF_HAND: EF-hand calcium-binding domain. 20..32
PROSITE EF_HAND: EF-hand calcium-binding domain. 56..68
PROSITE EF_HAND: EF-hand calcium-binding domain. 93..105
__________________
Plus Strand HSPs:
Score = 142 (50.0 bits), Expect = 5.3e-09, P = 5.3e-09
Identities = 30/78 (38%), Positives = 48/78 (61%), Frame = +1
Query: 49 FLRVFSKVMR---MNTEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ MR E + K FD+DG+G IS ELR+ + +G +L + +++I E
Sbjct: 68 FLTMMARKMRDTDSEEEIKEAFKVFDKDGNGYISAAELRHVMTNLGEKLTDNEVDEMIRE 127
Query: 220 LDSDGDGFLSLEDFVKLM 273
D DGDG ++ E+FVK+M
Sbjct: 128 ADIDGDGQINYEEFVKMM 145
Score = 119 (41.9 bits), Expect = 1.5e-06, P = 1.5e-06
Identities = 24/82 (29%), Positives = 47/82 (57%), Frame = +1
Query: 85 TEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFV 264
+EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 10 SEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFL 69
Query: 265 KLMEAAGED-EKLKDLEEAFEM 327
+M D + ++++EAF++
Sbjct: 70 TMMARKMRDTDSEEEIKEAFKV 91
Score = 75 (26.4 bits), Expect = 0.56, P = 0.43
Identities = 27/80 (33%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E L M+ ++ D+E+ I+E D DG+G++S
Sbjct: 47 ELQDMINEVDADGNGTIDFPEF---LTMMARKMRDTDSEEEIKEAFKVFDKDGNGYISAA 103
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D E EM
Sbjct: 104 ELRHVMTNLGE--KLTD-NEVDEM 124
>gi|4335787|gb|AAD17455.1| (U91642) calmodulin [Pleurotus ostreatus]
>gi|4335789|gb|AAD17456.1| (U91643) calmodulin [Pleurotus
ostreatus]
Length = 149
Frame 1 hits (HSPs): ______________________________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
Plus Strand HSPs:
Score = 142 (50.0 bits), Expect = 5.3e-09, P = 5.3e-09
Identities = 30/78 (38%), Positives = 48/78 (61%), Frame = +1
Query: 49 FLRVFSKVMR---MNTEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ MR E + K FD+DG+G IS ELR+ + +G +L + +++I E
Sbjct: 69 FLTMMARKMRDTDSEEEIKEAFKVFDKDGNGYISAAELRHVMTNLGEKLTDNEVDEMIRE 128
Query: 220 LDSDGDGFLSLEDFVKLM 273
D DGDG ++ E+FVK+M
Sbjct: 129 ADVDGDGQINYEEFVKMM 146
Score = 119 (41.9 bits), Expect = 1.5e-06, P = 1.5e-06
Identities = 24/82 (29%), Positives = 47/82 (57%), Frame = +1
Query: 85 TEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFV 264
+EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 11 SEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFL 70
Query: 265 KLMEAAGED-EKLKDLEEAFEM 327
+M D + ++++EAF++
Sbjct: 71 TMMARKMRDTDSEEEIKEAFKV 92
Score = 75 (26.4 bits), Expect = 0.57, P = 0.43
Identities = 27/80 (33%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E L M+ ++ D+E+ I+E D DG+G++S
Sbjct: 48 ELQDMINEVDADGNGTIDFPEF---LTMMARKMRDTDSEEEIKEAFKVFDKDGNGYISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D E EM
Sbjct: 105 ELRHVMTNLGE--KLTD-NEVDEM 125
>gi|4033509|sp|P02598|CALM_TETPY CALMODULIN >gi|2144831|pir||MCTE calmodulin -
Tetrahymena pyriformis >gi|7441480|pir||S28954 calmodulin -
Tetrahymena thermophila >gi|217405|dbj|BAA01391.1| (D10521)
calmodulin [Tetrahymena pyriformis]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
Annotated Domains: _________________________ _______________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
__________________
Annotated Domains:
BLOCKS BL00018: EF-hand calcium-binding domain 129..141
DOMO DM00011: CALMODULINREPEAT 1..32
Entrez acetylation site 2
Entrez methylation site: (TRI-). 116
Entrez Calcium binding region: SITE 1. 21..32
Entrez Calcium binding region: SITE 2. 57..68
Entrez Calcium binding region: SITE 3. 94..105
Entrez Calcium binding region: SITE 4. 130..141
PFAM efhand: EF hand 11..39
PFAM efhand: EF hand 47..75
PFAM efhand: EF hand 84..112
PFAM efhand: EF hand 120..148
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 5..71
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 83..144
PROSITE EF_HAND: EF-hand calcium-binding domain. 130..142
PROSITE EF_HAND: EF-hand calcium-binding domain. 21..33
PROSITE EF_HAND: EF-hand calcium-binding domain. 57..69
PROSITE EF_HAND: EF-hand calcium-binding domain. 94..106
__________________
Plus Strand HSPs:
Score = 142 (50.0 bits), Expect = 5.3e-09, P = 5.3e-09
Identities = 31/80 (38%), Positives = 52/80 (65%), Frame = +1
Query: 49 FLRVFSKVMR-MNTEFERV--LKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ M+ +TE E + K FD DG+G IS ELR+ + +G +L ++ +++I E
Sbjct: 69 FLSLMARKMKDTDTEEELIEAFKVFDRDGNGLISAAELRHVMTNLGEKLTDEEVDEMIRE 128
Query: 220 LDSDGDGFLSLEDFVKLMEA 279
D DGDG ++ E+FV++M A
Sbjct: 129 ADIDGDGHINYEEFVRMMMA 148
Score = 119 (41.9 bits), Expect = 1.5e-06, P = 1.5e-06
Identities = 26/81 (32%), Positives = 46/81 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLS 71
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
LM +D + ++L EAF++
Sbjct: 72 LMARKMKDTDTEEELIEAFKV 92
Score = 72 (25.3 bits), Expect = 2.1, P = 0.88
Identities = 28/80 (35%), Positives = 41/80 (51%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEK-LIEEL---DSDGDGFLSLE 255
E + ++ D DG+G I E L ++ ++ D E+ LIE D DG+G +S
Sbjct: 48 ELQDMINEVDADGNGTIDFPEF---LSLMARKMKDTDTEEELIEAFKVFDRDGNGLISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 105 ELRHVMTNLGE--KLTD-EEVDEM 125
>gi|6041859|gb|AAF02168.1|AC009853_28 (AC009853) putative calmodulin
[Arabidopsis thaliana]
Length = 153
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | | 153
0 50 100 150
Plus Strand HSPs:
Score = 141 (49.6 bits), Expect = 6.8e-09, P = 6.8e-09
Identities = 29/81 (35%), Positives = 52/81 (64%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
E R+ + FD +GDGKI+ EL + L +G + KD ++IE++D +GDG++ +E+F
Sbjct: 5 ELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGG 64
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
L + E+ ++ +D+ EAF +
Sbjct: 65 LYQTIMEERDEEEDMREAFNV 85
Score = 102 (35.9 bits), Expect = 9.3e-05, P = 9.3e-05
Identities = 22/68 (32%), Positives = 41/68 (60%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIG---GELLAKDAEKLIEELDSDGDGFLSLED 258
+ FD++ DG I+ ELR+ L +G G L +D +++I ++D DGDG ++ ++
Sbjct: 78 DMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTL-EDCKRMISKVDVDGDGMVNFKE 136
Query: 259 FVKLMEAAG 285
F ++M+ G
Sbjct: 137 FKQMMKGGG 145
>gi|4959165|gb|AAD34262.1|AF084414_1 (AF084414) calmodulin mutant SYNCAM57D
[synthetic construct]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
Plus Strand HSPs:
Score = 140 (49.3 bits), Expect = 8.7e-09, P = 8.7e-09
Identities = 32/80 (40%), Positives = 54/80 (67%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFERVLK----YFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIE 216
FL + ++VM+ +T+ E LK FD+DG+G IS ELR+ + +G +L ++ +++I
Sbjct: 69 FLNLMARVMK-DTDSEEELKEAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIR 127
Query: 217 ELDSDGDGFLSLEDFVKLMEA 279
E D DGDG ++ E+FV++M A
Sbjct: 128 EADVDGDGQVNYEEFVQVMMA 148
Score = 124 (43.7 bits), Expect = 4.3e-07, P = 4.3e-07
Identities = 26/81 (32%), Positives = 46/81 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLN 71
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
LM +D + ++L+EAF +
Sbjct: 72 LMARVMKDTDSEEELKEAFRV 92
Score = 80 (28.2 bits), Expect = 0.024, P = 0.024
Identities = 27/80 (33%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E N + + + D+E+ ++E D DG+GF+S
Sbjct: 48 ELQDMINEVDADGNGTIDFPEFLNLMARV---MKDTDSEEELKEAFRVFDKDGNGFISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 105 ELRHVMTNLGE--KLTD-EEVDEM 125
>gi|4959170|gb|AAD34267.1|AF084419_1 (AF084419) calmodulin mutant SYNCAM64A
[synthetic construct]
Length = 147
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 147
0 50 100
Plus Strand HSPs:
Score = 140 (49.3 bits), Expect = 8.7e-09, P = 8.7e-09
Identities = 28/78 (35%), Positives = 52/78 (66%), Frame = +1
Query: 49 FLRVFSKVMR-MNTEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELD 225
FL + ++ M+ ++E + + FD+DG+G IS ELR+ + +G +L ++ +++I E D
Sbjct: 69 FLNLMARKMKDTDSELKEAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREAD 128
Query: 226 SDGDGFLSLEDFVKLMEA 279
DGDG ++ E+FV++M A
Sbjct: 129 VDGDGQVNYEEFVQVMMA 146
Score = 121 (42.6 bits), Expect = 9.0e-07, P = 9.0e-07
Identities = 26/80 (32%), Positives = 44/80 (55%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLN 71
Query: 268 LMEAAGEDEKLKDLEEAFEM 327
LM +D +L+EAF +
Sbjct: 72 LMARKMKDTD-SELKEAFRV 90
Score = 76 (26.8 bits), Expect = 0.27, P = 0.24
Identities = 26/80 (32%), Positives = 40/80 (50%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAE--KLIEELDSDGDGFLSLEDF 261
E + ++ D DG+G I E N ++ ++ D+E + D DG+GF+S +
Sbjct: 48 ELQDMINEVDADGNGTIDFPEFLN---LMARKMKDTDSELKEAFRVFDKDGNGFISAAEL 104
Query: 262 VKLMEAAGEDEKLKDLEEAFEM 327
+M GE KL D EE EM
Sbjct: 105 RHVMTNLGE--KLTD-EEVDEM 123
>gi|1565285|emb|CAA69660.1| (Y08373) calmodulin [Toxoplasma gondii]
Length = 146
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 146
0 50 100
Plus Strand HSPs:
Score = 140 (49.3 bits), Expect = 8.7e-09, P = 8.7e-09
Identities = 31/78 (39%), Positives = 51/78 (65%), Frame = +1
Query: 49 FLRVFSKVMR-MNTEFERV--LKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ M+ +TE E + K FD DG+G IS ELR+ + +G +L ++ +++I E
Sbjct: 69 FLTLMARKMKDTDTEEELIEAFKVFDRDGNGFISAAELRHVMTNLGEKLTDEEVDEMIRE 128
Query: 220 LDSDGDGFLSLEDFVKLM 273
D DGDG ++ E+FVK+M
Sbjct: 129 ADVDGDGQINYEEFVKMM 146
Score = 118 (41.5 bits), Expect = 1.9e-06, P = 1.9e-06
Identities = 26/81 (32%), Positives = 46/81 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLT 71
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
LM +D + ++L EAF++
Sbjct: 72 LMARKMKDTDTEEELIEAFKV 92
Score = 76 (26.8 bits), Expect = 0.27, P = 0.23
Identities = 29/80 (36%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEK-LIEEL---DSDGDGFLSLE 255
E + ++ D DG+G I E L ++ ++ D E+ LIE D DG+GF+S
Sbjct: 48 ELQDMINEVDADGNGTIDFPEF---LTLMARKMKDTDTEEELIEAFKVFDRDGNGFISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 105 ELRHVMTNLGE--KLTD-EEVDEM 125
>gi|115519|sp|P27165|CALM_PHYIN CALMODULIN >gi|169306|gb|AAA21424.1| (M83535)
calmodulin [Phytophthora infestans]
Length = 149
Frame 1 hits (HSPs): ______________________________________________
Annotated Domains: _________________________ ________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
__________________
Annotated Domains:
BLOCKS BL00018: EF-hand calcium-binding domain 129..141
DOMO DM00011: CALMODULINREPEAT 1..32
Entrez Calcium binding region: SITE 1. 21..32
Entrez Calcium binding region: SITE 2. 57..68
Entrez Calcium binding region: SITE 3. 94..105
Entrez Calcium binding region: SITE 4. 130..141
PFAM efhand: EF hand 11..39
PFAM efhand: EF hand 47..75
PFAM efhand: EF hand 84..112
PFAM efhand: EF hand 120..148
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 5..71
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 81..144
PROSITE EF_HAND: EF-hand calcium-binding domain. 130..142
PROSITE EF_HAND: EF-hand calcium-binding domain. 21..33
PROSITE EF_HAND: EF-hand calcium-binding domain. 57..69
PROSITE EF_HAND: EF-hand calcium-binding domain. 94..106
__________________
Plus Strand HSPs:
Score = 140 (49.3 bits), Expect = 8.7e-09, P = 8.7e-09
Identities = 30/78 (38%), Positives = 50/78 (64%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFERVL---KYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ M+ E +L K FD+DG+G IS ELR+ + +G +L ++ +++I E
Sbjct: 69 FLTMMARKMKDTDSEEEILEAFKVFDKDGNGFISAAELRHIMTNLGEKLTDEEVDEMIRE 128
Query: 220 LDSDGDGFLSLEDFVKLM 273
D DGDG ++ E+FVK+M
Sbjct: 129 ADIDGDGQINYEEFVKMM 146
Score = 115 (40.5 bits), Expect = 3.9e-06, P = 3.9e-06
Identities = 24/81 (29%), Positives = 46/81 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLT 71
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
+M +D + +++ EAF++
Sbjct: 72 MMARKMKDTDSEEEILEAFKV 92
Score = 80 (28.2 bits), Expect = 0.024, P = 0.024
Identities = 29/80 (36%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E L M+ ++ D+E+ I E D DG+GF+S
Sbjct: 48 ELQDMINEVDADGNGTIDFPEF---LTMMARKMKDTDSEEEILEAFKVFDKDGNGFISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 105 ELRHIMTNLGE--KLTD-EEVDEM 125
>gi|1085679|pir||S53019 calmodulin - Macrocystis pyrifera
>gi|728609|emb|CAA59418.1| (X85091) calmodulin [Macrocystis
pyrifera]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
Annotated Domains: _____ _____ _____ _____
__________________________________________________
Database sequence: | | | | 149
0 50 100
__________________
Annotated Domains:
PROSITE EF_HAND: EF-hand calcium-binding domain. 130..142
PROSITE EF_HAND: EF-hand calcium-binding domain. 21..33
PROSITE EF_HAND: EF-hand calcium-binding domain. 57..69
PROSITE EF_HAND: EF-hand calcium-binding domain. 94..106
__________________
Plus Strand HSPs:
Score = 140 (49.3 bits), Expect = 8.7e-09, P = 8.7e-09
Identities = 30/80 (37%), Positives = 51/80 (63%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFERVL---KYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ M+ E ++ K FD+DG+G IS ELR+ + +G +L ++ +++I E
Sbjct: 69 FLTMMARKMKDTDSEEEIIEAFKVFDKDGNGFISAAELRHIMTNLGEKLTDEEVDEMIRE 128
Query: 220 LDSDGDGFLSLEDFVKLMEA 279
D DGDG ++ E+FVK+M A
Sbjct: 129 ADIDGDGQINYEEFVKMMMA 148
Score = 115 (40.5 bits), Expect = 3.9e-06, P = 3.9e-06
Identities = 24/81 (29%), Positives = 46/81 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLT 71
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
+M +D + +++ EAF++
Sbjct: 72 MMARKMKDTDSEEEIIEAFKV 92
Score = 80 (28.2 bits), Expect = 0.024, P = 0.024
Identities = 29/80 (36%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E L M+ ++ D+E+ I E D DG+GF+S
Sbjct: 48 ELQDMINEVDADGNGTIDFPEF---LTMMARKMKDTDSEEEIIEAFKVFDKDGNGFISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 105 ELRHIMTNLGE--KLTD-EEVDEM 125
>gi|7441482|pir||T07122 calmodulin CAM5 - soybean >gi|310563|gb|AAA33948.1|
(L19359) calmodulin [Glycine max] >gi|1583771|prf||2121384E
calmodulin [Glycine max]
Length = 150
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 150
0 50 100
Plus Strand HSPs:
Score = 140 (49.3 bits), Expect = 8.7e-09, P = 8.7e-09
Identities = 32/82 (39%), Positives = 50/82 (60%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFE---RVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + +K M+ E E K FD+D +G IS ELR+ + +G +L ++ E++IEE
Sbjct: 69 FLNLMAKKMKETDEEEDLKEAFKVFDKDQNGYISASELRHVMINLGEKLTDEEVEQMIEE 128
Query: 220 LDSDGDGFLSLEDFVKLMEAAG 285
D DGDG ++ ++FVK+M G
Sbjct: 129 ADLDGDGQVNYDEFVKMMMTIG 150
Score = 106 (37.3 bits), Expect = 3.5e-05, P = 3.5e-05
Identities = 24/82 (29%), Positives = 47/82 (57%), Frame = +1
Query: 85 TEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFV 264
+E + FD+DGDG I+ E + + ++ + +I E+D+DG+G + +F+
Sbjct: 11 SEIKEAFGLFDKDGDGCITVDEFVTVIRSLVQNPTEEELQDMINEVDADGNGTIEFVEFL 70
Query: 265 KLM-EAAGEDEKLKDLEEAFEM 327
LM + E ++ +DL+EAF++
Sbjct: 71 NLMAKKMKETDEEEDLKEAFKV 92
Score = 96 (33.8 bits), Expect = 0.00040, P = 0.00040
Identities = 23/84 (27%), Positives = 44/84 (52%), Frame = +1
Query: 85 TEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFV 264
+E + FD+DGDG I+ E + + ++ + +I E+D+DG+G + +F+
Sbjct: 11 SEIKEAFGLFDKDGDGCITVDEFVTVIRSLVQNPTEEELQDMINEVDADGNGTIEFVEFL 70
Query: 265 KLM----EAAGEDEKLKDLEEAFE 324
LM + E+E LK+ + F+
Sbjct: 71 NLMAKKMKETDEEEDLKEAFKVFD 94
Score = 68 (23.9 bits), Expect = 9.5, P = 1.0
Identities = 26/97 (26%), Positives = 47/97 (48%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFER--VLKYFDEDGDGKISPCELRNRLGMIGGEL-LAKDAEKLIEE 219
F+ V +++ TE E ++ D DG+G I E N + E +D ++ +
Sbjct: 33 FVTVIRSLVQNPTEEELQDMINEVDADGNGTIEFVEFLNLMAKKMKETDEEEDLKEAFKV 92
Query: 220 LDSDGDGFLSLEDFVKLMEAAGE---DEKLKDL-EEA 318
D D +G++S + +M GE DE+++ + EEA
Sbjct: 93 FDKDQNGYISASELRHVMINLGEKLTDEEVEQMIEEA 129
>gi|2129799|pir||S65149 pollen allergen Bra n I (clone 43 and others) - rape
>gi|7446010|pir||S65151 pollen allergen Bra r I (clone 5 and
others) - turnip >gi|1255536|dbj|BAA09632.1| (D63151)
Calcium-Binding Protein [Brassica napus]
>gi|1255540|dbj|BAA09634.1| (D63153) Calcium-Binding Protein
[Brassica rapa] >gi|7415719|dbj|BAA93509.1| (AB032260) pollen
calcium-binding protein [Brassica rapa]
Length = 79
Frame 1 hits (HSPs): _________________________________________________
Annotated Domains: ________ ________
__________________________________________________
Database sequence: | | | | | 79
0 20 40 60
__________________
Annotated Domains:
PROSITE EF_HAND: EF-hand calcium-binding domain. 14..26
PROSITE EF_HAND: EF-hand calcium-binding domain. 49..61
__________________
Plus Strand HSPs:
Score = 140 (49.3 bits), Expect = 8.7e-09, P = 8.7e-09
Identities = 29/80 (36%), Positives = 47/80 (58%), Frame = +1
Query: 82 NTEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDF 261
+ E ER+ K FD DGDGKIS EL L +G + D +++ ++D+DGDG +S ++F
Sbjct: 3 DAEHERIFKKFDTDGDGKISAAELEEALKKLGS-VTPDDVTRMMAKIDTDGDGNISFQEF 61
Query: 262 VKLMEAAGEDEKLKDLEEAF 321
+ A+ +KD+ + F
Sbjct: 62 TEF--ASANPGLMKDVAKVF 79
>gi|115516|sp|P02596|CALM_METSE CALMODULIN >gi|71672|pir||MCXAM calmodulin -
sea anemone (Metridium senile) (tentative sequence)
Length = 148
Frame 1 hits (HSPs): ______________________________________________
Annotated Domains: __________________________ _______________________
__________________________________________________
Database sequence: | | | | 148
0 50 100
__________________
Annotated Domains:
BLOCKS BL00018: EF-hand calcium-binding domain 129..141
DOMO DM00011: CALMODULINREPEAT 1..32
Entrez blocked site 1
Entrez methylation site: (TRI-). 115
Entrez Calcium binding region: SITE 1. 20..31
Entrez Calcium binding region: SITE 2. 56..67
Entrez Calcium binding region: SITE 3. 93..104
Entrez Calcium binding region: SITE 4. 129..140
PFAM efhand: EF hand 11..39
PFAM efhand: EF hand 47..75
PFAM efhand: EF hand 84..112
PFAM efhand: EF hand 120..148
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 5..71
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 81..144
PROSITE EF_HAND: EF-hand calcium-binding domain. 129..141
PROSITE EF_HAND: EF-hand calcium-binding domain. 20..32
PROSITE EF_HAND: EF-hand calcium-binding domain. 56..68
PROSITE EF_HAND: EF-hand calcium-binding domain. 93..105
__________________
Plus Strand HSPs:
Score = 139 (48.9 bits), Expect = 1.1e-08, P = 1.1e-08
Identities = 29/78 (37%), Positives = 48/78 (61%), Frame = +1
Query: 49 FLRVFSKVMR---MNTEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ M+ E + FD+DGDG IS ELR+ + +G +L ++ +++I E
Sbjct: 68 FLTMMARKMKDTDSEEEIREAFRVFDKDGDGFISAAELRHVMTNLGEKLTDEEVDEMIRE 127
Query: 220 LDSDGDGFLSLEDFVKLM 273
D DGDG ++ E+FVK+M
Sbjct: 128 ADIDGDGQVNYEEFVKMM 145
Score = 122 (42.9 bits), Expect = 7.0e-07, P = 7.0e-07
Identities = 25/81 (30%), Positives = 45/81 (55%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DGDG + +F+
Sbjct: 11 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLT 70
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
+M +D + +++ EAF +
Sbjct: 71 MMARKMKDTDSEEEIREAFRV 91
Score = 92 (32.4 bits), Expect = 0.0011, P = 0.0011
Identities = 31/80 (38%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DGDG I E L M+ ++ D+E+ I E D DGDGF+S
Sbjct: 47 ELQDMINEVDADGDGTIDFPEF---LTMMARKMKDTDSEEEIREAFRVFDKDGDGFISAA 103
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 104 ELRHVMTNLGE--KLTD-EEVDEM 124
>gi|71673|pir||MCJZR calmodulin - sea pansy (Renilla reniformis) (tentative
sequence)
Length = 147
Frame 1 hits (HSPs): ______________________________________________
Annotated Domains: _________________________ ________________________
__________________________________________________
Database sequence: | | | | 147
0 50 100
__________________
Annotated Domains:
Entrez domain: calmodulin repeat homology #labe 6..38
Entrez domain: calmodulin repeat homology #labe 42..74
Entrez domain: calmodulin repeat homology #labe 79..111
Entrez domain: calmodulin repeat homology #labe 115..147
Entrez modified site: blocked amino end (Ala) 1
Entrez binding site: calcium (Asp, Asp, Asp, Th 19
Entrez binding site: calcium (Asp, Asp, Asp, Th 21
Entrez binding site: calcium (Asp, Asp, Asp, Th 23
Entrez binding site: calcium (Asp, Asp, Asp, Th 25
Entrez binding site: calcium (Asp, Asp, Asp, Th 27
Entrez binding site: calcium (Asp, Asp, Asp, Th 30
Entrez binding site: calcium (Asp, Asp, Asx, Th 55
Entrez binding site: calcium (Asp, Asp, Asx, Th 57
Entrez binding site: calcium (Asp, Asp, Asx, Th 59
Entrez binding site: calcium (Asp, Asp, Asx, Th 61
Entrez binding site: calcium (Asp, Asp, Asx, Th 63
Entrez binding site: calcium (Asp, Asp, Asx, Th 66
Entrez binding site: calcium (Asp, Asp, Asp, Ph 92
Entrez binding site: calcium (Asp, Asp, Asp, Ph 94
Entrez binding site: calcium (Asp, Asp, Asp, Ph 96
Entrez binding site: calcium (Asp, Asp, Asp, Ph 98
Entrez binding site: calcium (Asp, Asp, Asp, Ph 100
Entrez binding site: calcium (Asp, Asp, Asp, Ph 103
Entrez modified site: N6,N6,N6-trimethyllysine 114
Entrez binding site: calcium (Asp, Asp, Asp, Gl 128
Entrez binding site: calcium (Asp, Asp, Asp, Gl 130
Entrez binding site: calcium (Asp, Asp, Asp, Gl 132
Entrez binding site: calcium (Asp, Asp, Asp, Gl 134
Entrez binding site: calcium (Asp, Asp, Asp, Gl 136
Entrez binding site: calcium (Asp, Asp, Asp, Gl 139
PROSITE EF_HAND: EF-hand calcium-binding domain. 128..140
PROSITE EF_HAND: EF-hand calcium-binding domain. 19..31
PROSITE EF_HAND: EF-hand calcium-binding domain. 92..104
__________________
Plus Strand HSPs:
Score = 139 (48.9 bits), Expect = 1.1e-08, P = 1.1e-08
Identities = 29/78 (37%), Positives = 48/78 (61%), Frame = +1
Query: 49 FLRVFSKVMR---MNTEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ M+ E + FD+DGDG IS ELR+ + +G +L ++ +++I E
Sbjct: 67 FLTMMARKMKDTDSEEEIREAFRVFDKDGDGFISAAELRHVMTNLGEKLTDEEVDEMIRE 126
Query: 220 LDSDGDGFLSLEDFVKLM 273
D DGDG ++ E+FVK+M
Sbjct: 127 ADIDGDGQVNYEEFVKMM 144
Score = 120 (42.2 bits), Expect = 1.1e-06, P = 1.1e-06
Identities = 24/81 (29%), Positives = 45/81 (55%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 10 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGBGTIDFPEFLT 69
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
+M +D + +++ EAF +
Sbjct: 70 MMARKMKDTDSEEEIREAFRV 90
Score = 90 (31.7 bits), Expect = 0.0017, P = 0.0017
Identities = 30/80 (37%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E L M+ ++ D+E+ I E D DGDGF+S
Sbjct: 46 ELQDMINEVDADGBGTIDFPEF---LTMMARKMKDTDSEEEIREAFRVFDKDGDGFISAA 102
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 103 ELRHVMTNLGE--KLTD-EEVDEM 123
>gi|7441484|pir||T06437 calmodulin - garden pea >gi|535428|gb|AAA92677.1|
(U13736) calmodulin-like protein [Pisum sativum]
Length = 150
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 150
0 50 100
Plus Strand HSPs:
Score = 139 (48.9 bits), Expect = 1.1e-08, P = 1.1e-08
Identities = 30/80 (37%), Positives = 49/80 (61%), Frame = +1
Query: 49 FLRVFSKVMR---MNTEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ M+ E K FD+D +G ISP ELR+ + +G +L ++ +++I+E
Sbjct: 69 FLNLMARKMKDTDAEEELREAFKVFDKDQNGYISPSELRHVMMNLGEKLSDEEVKQMIKE 128
Query: 220 LDSDGDGFLSLEDFVKLMEA 279
D DGDG + +DFVK+M A
Sbjct: 129 ADMDGDGQVDYDDFVKMMMA 148
Score = 111 (39.1 bits), Expect = 1.0e-05, P = 1.0e-05
Identities = 23/81 (28%), Positives = 47/81 (58%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
+F+ FD+DGDG ++ EL + + ++ + +I E+D+DG+G + ++F+
Sbjct: 12 DFKEAFGLFDKDGDGCVTVEELATVIRSLDQNPTEEELQDMISEVDADGNGTIEFDEFLN 71
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
LM +D + ++L EAF++
Sbjct: 72 LMARKMKDTDAEEELREAFKV 92
Score = 69 (24.3 bits), Expect = 6.8, P = 1.0
Identities = 25/80 (31%), Positives = 41/80 (51%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E L ++ ++ DAE+ + E D D +G++S
Sbjct: 48 ELQDMISEVDADGNGTI---EFDEFLNLMARKMKDTDAEEELREAFKVFDKDQNGYISPS 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE +M
Sbjct: 105 ELRHVMMNLGE--KLSD-EEVKQM 125
>gi|3800851|gb|AAC68892.1| (AF081671) VU91D calmodulin [synthetic construct]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
Plus Strand HSPs:
Score = 139 (48.9 bits), Expect = 1.1e-08, P = 1.1e-08
Identities = 32/82 (39%), Positives = 51/82 (62%), Frame = +1
Query: 43 SIFLRVFSKVMRMNTEFERVL---KYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLI 213
S FL + S+ ++ N + +L K FD++GDG IS EL++ L IG +L + + +I
Sbjct: 67 SEFLALMSRQLKSNDSEQELLEAFKVFDKNGDGLISAAELKHVLTSIGEKLTDAEVDDMI 126
Query: 214 EELDSDGDGFLSLEDFVKLMEA 279
E D DGDG ++ E+FV++M A
Sbjct: 127 READVDGDGQVNYEEFVQVMMA 148
Score = 106 (37.3 bits), Expect = 3.5e-05, P = 3.5e-05
Identities = 25/81 (30%), Positives = 42/81 (51%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG IS EL + +G + L+ E+D DG+ + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTISSSELATVMRSLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLA 71
Query: 268 LMEAA-GEDEKLKDLEEAFEM 327
LM ++ ++L EAF++
Sbjct: 72 LMSRQLKSNDSEQELLEAFKV 92
Score = 70 (24.6 bits), Expect = 4.8, P = 0.99
Identities = 23/75 (30%), Positives = 40/75 (53%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEK-LIEEL---DSDGDGFLSLE 255
E ++ D DG+ +I E L ++ +L + D+E+ L+E D +GDG +S
Sbjct: 48 EVNDLMNEIDVDGNHQI---EFSEFLALMSRQLKSNDSEQELLEAFKVFDKNGDGLISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLE 312
+ ++ + GE KL D E
Sbjct: 105 ELKHVLTSIGE--KLTDAE 121
>gi|1864024|gb|AAC49648.1| (U35683) calcium-binding pollen allergen [Cynodon
dactylon] >gi|1871507|emb|CAA62634.1| (X91256) calcium-binding
pollen allergen [Cynodon dactylon]
Length = 82
Frame 1 hits (HSPs): __________________________________________________
__________________________________________________
Database sequence: | | | | | | 82
0 20 40 60 80
Plus Strand HSPs:
Score = 139 (48.9 bits), Expect = 1.1e-08, P = 1.1e-08
Identities = 29/85 (34%), Positives = 48/85 (56%), Frame = +1
Query: 67 KVMRMNTEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFL 246
K M + E + K FD +GDGKIS EL + L +G A + ++++ E+D+DGDGF+
Sbjct: 1 KTMADTGDMEHIFKRFDTNGDGKISLAELTDALRTLGSTS-ADEVQRMMAEIDTDGDGFI 59
Query: 247 SLEDFVKLMEAAGEDEKLKDLEEAF 321
++F+ A +KD+ + F
Sbjct: 60 DFDEFISFCNA--NPGLMKDVAKVF 82
>gi|3337403|gb|AAD05375.1| (AF015810) calcium-binding pollen allergen [Olea
europaea]
Length = 84
Frame 1 hits (HSPs): _____________________________________________
__________________________________________________
Database sequence: | | | | | | 84
0 20 40 60 80
Plus Strand HSPs:
Score = 139 (48.9 bits), Expect = 1.1e-08, P = 1.1e-08
Identities = 30/78 (38%), Positives = 47/78 (60%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
E ER+ K FD +GDGKIS EL L +G + ++ ++++ E+D+DGDGF+S E+F
Sbjct: 10 EHERIFKRFDANGDGKISSSELGETLKTLGS-VTPEEIQRMMAEIDTDGDGFISFEEFTV 68
Query: 268 LMEAAGEDEKLKDLEEAF 321
A +KD+ + F
Sbjct: 69 FARA--NRGLVKDVAKIF 84
>gi|3367732|emb|CAA76887.1| (Y17835) p7 protein [Phleum pratense]
Length = 78
Frame 1 hits (HSPs): __________________________________________________
__________________________________________________
Database sequence: | | | | | 78
0 20 40 60
Plus Strand HSPs:
Score = 139 (48.9 bits), Expect = 1.1e-08, P = 1.1e-08
Identities = 29/81 (35%), Positives = 47/81 (58%), Frame = +1
Query: 79 MNTEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLED 258
M + ER+ K FD +GDGKIS EL + L +G A + ++++ E+D+DGDGF+ +
Sbjct: 1 MADDMERIFKRFDTNGDGKISLSELTDALRTLGSTS-ADEVQRMMAEIDTDGDGFIDFNE 59
Query: 259 FVKLMEAAGEDEKLKDLEEAF 321
F+ A +KD+ + F
Sbjct: 60 FISFCNA--NPGLMKDVAKVF 78
>gi|6017120|gb|AAF01603.1|AC009895_24 (AC009895) pollen allergen Bra r II
[Arabidopsis thaliana]
Length = 83
Frame 1 hits (HSPs): ______________________________________________
__________________________________________________
Database sequence: | | | | | | 83
0 20 40 60 80
Plus Strand HSPs:
Score = 139 (48.9 bits), Expect = 1.1e-08, P = 1.1e-08
Identities = 28/78 (35%), Positives = 50/78 (64%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
E +R+ K FD +GDGKIS EL + L +G + +D ++++ E+D+DGDG++S ++F+
Sbjct: 9 EHDRIFKKFDANGDGKISAAELGDALKNLGS-VTHEDIKRMMAEIDTDGDGYISYQEFID 67
Query: 268 LMEAAGEDEKLKDLEEAF 321
A+ +KD+ + F
Sbjct: 68 F--ASANRGLMKDVAKIF 83
>gi|4959648|gb|AAD34437.1|AF084453_1 (AF084453) calmodulin mutant SYNCAM34
[synthetic construct]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
Plus Strand HSPs:
Score = 138 (48.6 bits), Expect = 1.4e-08, P = 1.4e-08
Identities = 31/80 (38%), Positives = 54/80 (67%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFERVLK----YFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIE 216
FL + ++ M+ +T+ E LK FD+DG+G IS ELR+ + +G +L ++ +++I
Sbjct: 69 FLNLMARKMK-DTDSEEELKEAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIR 127
Query: 217 ELDSDGDGFLSLEDFVKLMEA 279
E D DGDG+++ E+FV++M A
Sbjct: 128 EADVDGDGWVNYEEFVQVMMA 148
Score = 123 (43.3 bits), Expect = 5.5e-07, P = 5.5e-07
Identities = 26/81 (32%), Positives = 46/81 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLN 71
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
LM +D + ++L+EAF +
Sbjct: 72 LMARKMKDTDSEEELKEAFRV 92
Score = 80 (28.2 bits), Expect = 0.024, P = 0.024
Identities = 27/80 (33%), Positives = 43/80 (53%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E N ++ ++ D+E+ ++E D DG+GF+S
Sbjct: 48 ELQDMINEVDADGNGTIDFPEFLN---LMARKMKDTDSEEELKEAFRVFDKDGNGFISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 105 ELRHVMTNLGE--KLTD-EEVDEM 125
>gi|115520|sp|P24044|CALM_PLAFA CALMODULIN >gi|1070616|pir||MCZQF calmodulin -
Plasmodium falciparum >gi|160128|gb|AAA29510.1| (M59770) calmodulin
[Plasmodium falciparum] >gi|385234|gb|AAA29508.1| (M99442)
calmodulin [Plasmodium falciparum]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
Annotated Domains: __________________________ _______________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
__________________
Annotated Domains:
BLOCKS BL00018: EF-hand calcium-binding domain 130..142
DOMO DM00011: CALMODULINREPEAT 1..33
Entrez Calcium binding region: SITE 1. 21..32
Entrez Calcium binding region: SITE 2. 57..68
Entrez Calcium binding region: SITE 3. 94..105
Entrez Calcium binding region: SITE 4. 130..141
PFAM efhand: EF hand 12..40
PFAM efhand: EF hand 48..76
PFAM efhand: EF hand 85..113
PFAM efhand: EF hand 121..149
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 6..72
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 84..145
PROSITE EF_HAND: EF-hand calcium-binding domain. 130..142
PROSITE EF_HAND: EF-hand calcium-binding domain. 21..33
PROSITE EF_HAND: EF-hand calcium-binding domain. 57..69
PROSITE EF_HAND: EF-hand calcium-binding domain. 94..106
__________________
Plus Strand HSPs:
Score = 138 (48.6 bits), Expect = 1.4e-08, P = 1.4e-08
Identities = 31/80 (38%), Positives = 52/80 (65%), Frame = +1
Query: 49 FLRVFSKVMR-MNTEFERV--LKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ ++ +TE E + + FD DGDG IS ELR+ + +G +L ++ +++I E
Sbjct: 69 FLTLMARKLKDTDTEEELIEAFRVFDRDGDGYISADELRHVMTNLGEKLTNEEVDEMIRE 128
Query: 220 LDSDGDGFLSLEDFVKLMEA 279
D DGDG ++ E+FVK+M A
Sbjct: 129 ADIDGDGQINYEEFVKMMIA 148
Score = 118 (41.5 bits), Expect = 1.9e-06, P = 1.9e-06
Identities = 26/82 (31%), Positives = 46/82 (56%), Frame = +1
Query: 85 TEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFV 264
+EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 11 SEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEIDTDGNGTIDFPEFL 70
Query: 265 KLMEAAGED-EKLKDLEEAFEM 327
LM +D + ++L EAF +
Sbjct: 71 TLMARKLKDTDTEEELIEAFRV 92
Score = 79 (27.8 bits), Expect = 0.031, P = 0.031
Identities = 29/80 (36%), Positives = 43/80 (53%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEK-LIEEL---DSDGDGFLSLE 255
E + ++ D DG+G I E L ++ +L D E+ LIE D DGDG++S +
Sbjct: 48 ELQDMINEIDTDGNGTIDFPEF---LTLMARKLKDTDTEEELIEAFRVFDRDGDGYISAD 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL + EE EM
Sbjct: 105 ELRHVMTNLGE--KLTN-EEVDEM 125
>gi|115510|sp|P11118|CALM_EUGGR CALMODULIN >gi|71681|pir||MCEG calmodulin -
Euglena gracilis
Length = 148
Frame 1 hits (HSPs): ______________________________________________
Annotated Domains: __________________________ _______________________
__________________________________________________
Database sequence: | | | | 148
0 50 100
__________________
Annotated Domains:
BLOCKS BL00018: EF-hand calcium-binding domain 129..141
DOMO DM00011: CALMODULINREPEAT 1..32
Entrez acetylation site 1
Entrez methylation site 115
Entrez methylation site 148
Entrez Calcium binding region: SITE 1. 20..31
Entrez Calcium binding region: SITE 2. 56..67
Entrez Calcium binding region: SITE 3. 93..104
Entrez Calcium binding region: SITE 4. 129..140
PFAM efhand: EF hand 11..39
PFAM efhand: EF hand 47..75
PFAM efhand: EF hand 84..112
PFAM efhand: EF hand 120..148
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 5..71
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 81..144
PROSITE EF_HAND: EF-hand calcium-binding domain. 129..141
PROSITE EF_HAND: EF-hand calcium-binding domain. 20..32
PROSITE EF_HAND: EF-hand calcium-binding domain. 56..68
PROSITE EF_HAND: EF-hand calcium-binding domain. 93..105
__________________
Plus Strand HSPs:
Score = 138 (48.6 bits), Expect = 1.4e-08, P = 1.4e-08
Identities = 31/78 (39%), Positives = 50/78 (64%), Frame = +1
Query: 49 FLRVFSKVMR-MNTEFE--RVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + S+ M +TE E + FD+DG+G IS ELR+ + +G +L ++ +++I E
Sbjct: 68 FLTLMSRKMHDTDTEEEIKEAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIRE 127
Query: 220 LDSDGDGFLSLEDFVKLM 273
D DGDG ++ E+FVK+M
Sbjct: 128 ADVDGDGQINYEEFVKMM 145
Score = 117 (41.2 bits), Expect = 2.4e-06, P = 2.4e-06
Identities = 25/81 (30%), Positives = 43/81 (53%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D DG G + +F+
Sbjct: 11 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDQDGSGTIDFPEFLT 70
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
LM D + ++++EAF +
Sbjct: 71 LMSRKMHDTDTEEEIKEAFRV 91
Score = 80 (28.2 bits), Expect = 0.023, P = 0.023
Identities = 28/80 (35%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D+DG G I E L ++ ++ D E+ I+E D DG+GF+S
Sbjct: 47 ELQDMINEVDQDGSGTIDFPEF---LTLMSRKMHDTDTEEEIKEAFRVFDKDGNGFISAA 103
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 104 ELRHVMTNLGE--KLTD-EEVDEM 124
>gi|115480|sp|P25854|CAL1_ARATH CALMODULIN-1 >gi|166649|gb|AAA32762.1| (M38379)
calmodulin-1 [Arabidopsis thaliana]
Length = 136
Frame 1 hits (HSPs): ________________________________________________
Annotated Domains: __________ ___________ ____________ ___________
__________________________________________________
Database sequence: | | | | 136
0 50 100
__________________
Annotated Domains:
Entrez methylation site: (BY SIMILARITY). 103
Entrez Calcium binding region: SITE 1. 8..19
Entrez Calcium binding region: SITE 2. 44..55
Entrez Calcium binding region: SITE 3. 81..92
Entrez Calcium binding region: SITE 4. 117..128
PFAM efhand: EF hand 1..27
PFAM efhand: EF hand 35..63
PFAM efhand: EF hand 72..100
PFAM efhand: EF hand 108..136
PROSITE EF_HAND: EF-hand calcium-binding domain. 117..129
PROSITE EF_HAND: EF-hand calcium-binding domain. 44..56
PROSITE EF_HAND: EF-hand calcium-binding domain. 8..20
PROSITE EF_HAND: EF-hand calcium-binding domain. 81..93
__________________
Plus Strand HSPs:
Score = 138 (48.6 bits), Expect = 1.4e-08, P = 1.4e-08
Identities = 33/80 (41%), Positives = 52/80 (65%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFERVLK----YFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIE 216
FL + +K M+ +T+ E LK FD+D +G IS ELR+ + +G +L ++ E++I
Sbjct: 56 FLNLMAKKMK-DTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEEMIR 114
Query: 217 ELDSDGDGFLSLEDFVKLMEA 279
E D DGDG ++ E+FVK+M A
Sbjct: 115 EADVDGDGQINYEEFVKIMMA 135
Score = 110 (38.7 bits), Expect = 1.3e-05, P = 1.3e-05
Identities = 24/73 (32%), Positives = 43/73 (58%), Frame = +1
Query: 112 FDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVKLMEAAGED 291
FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+ LM +D
Sbjct: 7 FDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMAKKMKD 66
Query: 292 -EKLKDLEEAFEM 327
+ ++L+EAF +
Sbjct: 67 TDSEEELKEAFRV 79
Score = 72 (25.3 bits), Expect = 1.6, P = 0.80
Identities = 26/80 (32%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E N ++ ++ D+E+ ++E D D +GF+S
Sbjct: 35 ELQDMINEVDADGNGTIDFPEFLN---LMAKKMKDTDSEEELKEAFRVFDKDQNGFISAA 91
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 92 ELRHVMTNLGE--KLTD-EEVEEM 112
>gi|228408|prf||1803520B calmodulin 1 [Arabidopsis thaliana]
Length = 137
Frame 1 hits (HSPs): ________________________________________________
__________________________________________________
Database sequence: | | | | 137
0 50 100
Plus Strand HSPs:
Score = 138 (48.6 bits), Expect = 1.4e-08, P = 1.4e-08
Identities = 33/80 (41%), Positives = 52/80 (65%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFERVLK----YFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIE 216
FL + +K M+ +T+ E LK FD+D +G IS ELR+ + +G +L ++ E++I
Sbjct: 57 FLNLMAKKMK-DTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEEMIR 115
Query: 217 ELDSDGDGFLSLEDFVKLMEA 279
E D DGDG ++ E+FVK+M A
Sbjct: 116 EADVDGDGQINYEEFVKIMMA 136
Score = 110 (38.7 bits), Expect = 1.3e-05, P = 1.3e-05
Identities = 24/73 (32%), Positives = 43/73 (58%), Frame = +1
Query: 112 FDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVKLMEAAGED 291
FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+ LM +D
Sbjct: 8 FDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMAKKMKD 67
Query: 292 -EKLKDLEEAFEM 327
+ ++L+EAF +
Sbjct: 68 TDSEEELKEAFRV 80
Score = 72 (25.3 bits), Expect = 1.6, P = 0.81
Identities = 26/80 (32%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E N ++ ++ D+E+ ++E D D +GF+S
Sbjct: 36 ELQDMINEVDADGNGTIDFPEFLN---LMAKKMKDTDSEEELKEAFRVFDKDQNGFISAA 92
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 93 ELRHVMTNLGE--KLTD-EEVEEM 113
>gi|6580549|emb|CAB63264.1| (AJ251808) calcium-binding protein [Lotus
japonicus]
Length = 150
Frame 1 hits (HSPs): ________________________________________________
__________________________________________________
Database sequence: | | | | 150
0 50 100
Plus Strand HSPs:
Score = 138 (48.6 bits), Expect = 1.4e-08, P = 1.4e-08
Identities = 29/82 (35%), Positives = 54/82 (65%), Frame = +1
Query: 85 TEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFV 264
TE +RV + FD +GDG+I+ EL + L +G + K+ ++IE +D +GDG + +++F
Sbjct: 4 TELKRVFQMFDRNGDGRITKKELNDSLENLGIFIPDKELTQMIERIDVNGDGCVYIDEFG 63
Query: 265 KLMEAA-GEDEKLKDLEEAFEM 327
+L ++ E ++ KD++EAF +
Sbjct: 64 ELYQSIMDERDEEKDMKEAFNV 85
Score = 104 (36.6 bits), Expect = 5.7e-05, P = 5.7e-05
Identities = 23/68 (33%), Positives = 39/68 (57%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIG---GELLAKDAEKLIEELDSDGDGFLSLED 258
+ + FD++GDG I+ ELR L +G G + +D +K+I +D DGDG + +
Sbjct: 78 DMKEAFNVFDQNGDGFITVEELRTVLASLGIKQGRTV-EDCKKMIMNVDVDGDGMVDYHE 136
Query: 259 FVKLMEAAG 285
F ++M+ G
Sbjct: 137 FKQIMKGGG 145
>gi|9874|emb|CAA40264.1| (X56950) calmodulin [Plasmodium falciparum]
>gi|160126|gb|AAA29509.1| (M59349) calmodulin [Plasmodium
falciparum]
Length = 146
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 146
0 50 100
Plus Strand HSPs:
Score = 138 (48.6 bits), Expect = 1.4e-08, P = 1.4e-08
Identities = 31/80 (38%), Positives = 52/80 (65%), Frame = +1
Query: 49 FLRVFSKVMR-MNTEFERV--LKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ ++ +TE E + + FD DGDG IS ELR+ + +G +L ++ +++I E
Sbjct: 66 FLTLMARKLKDTDTEEELIEAFRVFDRDGDGYISADELRHVMTNLGEKLTNEEVDEMIRE 125
Query: 220 LDSDGDGFLSLEDFVKLMEA 279
D DGDG ++ E+FVK+M A
Sbjct: 126 ADIDGDGQINYEEFVKMMIA 145
Score = 101 (35.6 bits), Expect = 0.00012, P = 0.00012
Identities = 25/81 (30%), Positives = 44/81 (54%), Frame = +1
Query: 85 TEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFV 264
+EF+ FD+DGDG EL + +G + + +I E+D+DG+G + +F+
Sbjct: 11 SEFKEAFSLFDKDGDGTK---ELGTVMRSLGQNPTEAELQDMINEIDTDGNGTIDFPEFL 67
Query: 265 KLMEAAGED-EKLKDLEEAFEM 327
LM +D + ++L EAF +
Sbjct: 68 TLMARKLKDTDTEEELIEAFRV 89
Score = 79 (27.8 bits), Expect = 0.029, P = 0.028
Identities = 29/80 (36%), Positives = 43/80 (53%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEK-LIEEL---DSDGDGFLSLE 255
E + ++ D DG+G I E L ++ +L D E+ LIE D DGDG++S +
Sbjct: 45 ELQDMINEIDTDGNGTIDFPEF---LTLMARKLKDTDTEEELIEAFRVFDRDGDGYISAD 101
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL + EE EM
Sbjct: 102 ELRHVMTNLGE--KLTN-EEVDEM 122
>gi|4959645|gb|AAD34434.1|AF084450_1 (AF084450) calmodulin mutant SYNCAM31
[synthetic construct]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
Plus Strand HSPs:
Score = 137 (48.2 bits), Expect = 1.8e-08, P = 1.8e-08
Identities = 31/80 (38%), Positives = 54/80 (67%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFERVLK----YFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIE 216
FL + ++ M+ +T++E LK FD+DG+G IS ELR+ + +G +L ++ +++I
Sbjct: 69 FLNLMARKMK-DTDWEEELKEAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIR 127
Query: 217 ELDSDGDGFLSLEDFVKLMEA 279
E D DGDG ++ E+FV++M A
Sbjct: 128 EADVDGDGQVNYEEFVQVMMA 148
Score = 120 (42.2 bits), Expect = 1.1e-06, P = 1.1e-06
Identities = 26/81 (32%), Positives = 45/81 (55%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLN 71
Query: 268 LMEAAGEDEKLKD-LEEAFEM 327
LM +D ++ L+EAF +
Sbjct: 72 LMARKMKDTDWEEELKEAFRV 92
Score = 76 (26.8 bits), Expect = 0.28, P = 0.25
Identities = 27/80 (33%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E N ++ ++ D E+ ++E D DG+GF+S
Sbjct: 48 ELQDMINEVDADGNGTIDFPEFLN---LMARKMKDTDWEEELKEAFRVFDKDGNGFISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 105 ELRHVMTNLGE--KLTD-EEVDEM 125
>gi|71676|pir||MCDO calmodulin - slime mold (Dictyostelium discoideum)
Length = 151
Frame 1 hits (HSPs): ______________________________________________
Annotated Domains: _ ________________________________________________
__________________________________________________
Database sequence: | | | || 151
0 50 100 150
__________________
Annotated Domains:
Entrez domain: calmodulin repeat homology #labe 9..41
Entrez domain: calmodulin repeat homology #labe 45..77
Entrez domain: calmodulin repeat homology #labe 82..114
Entrez domain: calmodulin repeat homology #labe 118..150
Entrez modified site: blocked amino end 1
Entrez binding site: calcium (Asp, Asp, Asp, Se 22
Entrez binding site: calcium (Asp, Asp, Asp, Se 24
Entrez binding site: calcium (Asp, Asp, Asp, Se 26
Entrez binding site: calcium (Asp, Asp, Asp, Se 28
Entrez binding site: calcium (Asp, Asp, Asp, Se 30
Entrez binding site: calcium (Asp, Asp, Asp, Se 33
Entrez binding site: calcium (Asp, Asp, Asn, As 58
Entrez binding site: calcium (Asp, Asp, Asn, As 60
Entrez binding site: calcium (Asp, Asp, Asn, As 62
Entrez binding site: calcium (Asp, Asp, Asn, As 64
Entrez binding site: calcium (Asp, Asp, Asn, As 66
Entrez binding site: calcium (Asp, Asp, Asn, As 69
Entrez binding site: calcium (Asp, Asp, Asn, Ty 95
Entrez binding site: calcium (Asp, Asp, Asn, Ty 97
Entrez binding site: calcium (Asp, Asp, Asn, Ty 99
Entrez binding site: calcium (Asp, Asp, Asn, Ty 101
Entrez binding site: calcium (Asp, Asp, Asn, Ty 103
Entrez binding site: calcium (Asp, Asp, Asn, Ty 106
Entrez modified site: N6,N6,N6-trimethyllysine 117
Entrez binding site: calcium (Asp, Asp, Asp, Gl 131
Entrez binding site: calcium (Asp, Asp, Asp, Gl 133
Entrez binding site: calcium (Asp, Asp, Asp, Gl 135
Entrez binding site: calcium (Asp, Asp, Asp, Gl 137
Entrez binding site: calcium (Asp, Asp, Asp, Gl 139
Entrez binding site: calcium (Asp, Asp, Asp, Gl 142
PROSITE EF_HAND: EF-hand calcium-binding domain. 131..143
PROSITE EF_HAND: EF-hand calcium-binding domain. 22..34
PROSITE EF_HAND: EF-hand calcium-binding domain. 58..70
PROSITE EF_HAND: EF-hand calcium-binding domain. 95..107
__________________
Plus Strand HSPs:
Score = 137 (48.2 bits), Expect = 1.8e-08, P = 1.8e-08
Identities = 30/78 (38%), Positives = 51/78 (65%), Frame = +1
Query: 49 FLRVFSKVMR-MNTEFE--RVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ M+ +TE E K FD+DG+G IS ELR+ + +G +L ++ +++I E
Sbjct: 70 FLTMMARKMQDTDTEEEIREAFKVFDKDGNGYISAAELRHVMTSLGEKLTNEEVDEMIRE 129
Query: 220 LDSDGDGFLSLEDFVKLM 273
D DGDG ++ ++FVK+M
Sbjct: 130 ADLDGDGQVNYDEFVKMM 147
Score = 118 (41.5 bits), Expect = 1.9e-06, P = 1.9e-06
Identities = 24/81 (29%), Positives = 46/81 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 13 EFKEAFSLFDKDGDGSITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGNIDFPEFLT 72
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
+M +D + +++ EAF++
Sbjct: 73 MMARKMQDTDTEEEIREAFKV 93
Score = 77 (27.1 bits), Expect = 0.17, P = 0.16
Identities = 27/80 (33%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E L M+ ++ D E+ I E D DG+G++S
Sbjct: 49 ELQDMINEVDADGNGNIDFPEF---LTMMARKMQDTDTEEEIREAFKVFDKDGNGYISAA 105
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M + GE KL + EE EM
Sbjct: 106 ELRHVMTSLGE--KLTN-EEVDEM 126
>gi|167676|gb|AAA33171.1| (M13009) calmodulin [Dictyostelium discoideum]
Length = 139
Frame 1 hits (HSPs): _________________________________________________
__________________________________________________
Database sequence: | | | | 139
0 50 100
Plus Strand HSPs:
Score = 137 (48.2 bits), Expect = 1.8e-08, P = 1.8e-08
Identities = 30/78 (38%), Positives = 51/78 (65%), Frame = +1
Query: 49 FLRVFSKVMR-MNTEFE--RVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ M+ +TE E K FD+DG+G IS ELR+ + +G +L ++ +++I E
Sbjct: 58 FLTMMARKMQDTDTEEEIREAFKVFDKDGNGYISAAELRHVMTSLGEKLTNEEVDEMIRE 117
Query: 220 LDSDGDGFLSLEDFVKLM 273
D DGDG ++ ++FVK+M
Sbjct: 118 ADLDGDGQVNYDEFVKMM 135
Score = 118 (41.5 bits), Expect = 1.9e-06, P = 1.9e-06
Identities = 24/81 (29%), Positives = 46/81 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 1 EFKEAFSLFDKDGDGSITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGNIDFPEFLT 60
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
+M +D + +++ EAF++
Sbjct: 61 MMARKMQDTDTEEEIREAFKV 81
Score = 77 (27.1 bits), Expect = 0.13, P = 0.12
Identities = 27/80 (33%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E L M+ ++ D E+ I E D DG+G++S
Sbjct: 37 ELQDMINEVDADGNGNIDFPEF---LTMMARKMQDTDTEEEIREAFKVFDKDGNGYISAA 93
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M + GE KL + EE EM
Sbjct: 94 ELRHVMTSLGE--KLTN-EEVDEM 114
>gi|461684|sp|P02599|CALM_DICDI CALMODULIN >gi|290034|gb|AAA33172.1| (M64089)
calmodulin [Dictyostelium discoideum]
Length = 152
Frame 1 hits (HSPs): _____________________________________________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | || 152
0 50 100 150
__________________
Annotated Domains:
BLOCKS BL00018: EF-hand calcium-binding domain 131..143
DOMO DM00011: CALMODULINREPEAT 1..34
Entrez blocked site 2
Entrez Calcium binding region: SITE 1. 23..34
Entrez Calcium binding region: SITE 2. 59..70
Entrez Calcium binding region: SITE 3. 96..107
Entrez Calcium binding region: SITE 4. 132..143
PFAM efhand: EF hand 13..41
PFAM efhand: EF hand 49..77
PFAM efhand: EF hand 86..114
PFAM efhand: EF hand 122..150
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 7..73
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 83..146
PROSITE EF_HAND: EF-hand calcium-binding domain. 132..144
PROSITE EF_HAND: EF-hand calcium-binding domain. 23..35
PROSITE EF_HAND: EF-hand calcium-binding domain. 59..71
PROSITE EF_HAND: EF-hand calcium-binding domain. 96..108
__________________
Plus Strand HSPs:
Score = 137 (48.2 bits), Expect = 1.8e-08, P = 1.8e-08
Identities = 30/78 (38%), Positives = 51/78 (65%), Frame = +1
Query: 49 FLRVFSKVMR-MNTEFE--RVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ M+ +TE E K FD+DG+G IS ELR+ + +G +L ++ +++I E
Sbjct: 71 FLTMMARKMQDTDTEEEIREAFKVFDKDGNGYISAAELRHVMTSLGEKLTNEEVDEMIRE 130
Query: 220 LDSDGDGFLSLEDFVKLM 273
D DGDG ++ ++FVK+M
Sbjct: 131 ADLDGDGQVNYDEFVKMM 148
Score = 118 (41.5 bits), Expect = 1.9e-06, P = 1.9e-06
Identities = 24/81 (29%), Positives = 46/81 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 14 EFKEAFSLFDKDGDGSITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGNIDFPEFLT 73
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
+M +D + +++ EAF++
Sbjct: 74 MMARKMQDTDTEEEIREAFKV 94
Score = 77 (27.1 bits), Expect = 0.17, P = 0.16
Identities = 27/80 (33%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E L M+ ++ D E+ I E D DG+G++S
Sbjct: 50 ELQDMINEVDADGNGNIDFPEF---LTMMARKMQDTDTEEEIREAFKVFDKDGNGYISAA 106
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M + GE KL + EE EM
Sbjct: 107 ELRHVMTSLGE--KLTN-EEVDEM 127
>gi|115509|sp|P02594|CALM_ELEEL CALMODULIN >gi|71665|pir||MCEE calmodulin -
electric eel >gi|213130|gb|AAA49236.1| (M36168) calmodulin
[Electrophorus electricus]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
Annotated Domains: _________________________ ________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
__________________
Annotated Domains:
BLOCKS BL00018: EF-hand calcium-binding domain 129..141
DOMO DM00011: CALMODULINREPEAT 1..32
Entrez Calcium binding region: SITE 1. 21..32
Entrez Calcium binding region: SITE 2. 57..68
Entrez Calcium binding region: SITE 3. 94..105
Entrez Calcium binding region: SITE 4. 130..141
PFAM efhand: EF hand 11..39
PFAM efhand: EF hand 47..75
PFAM efhand: EF hand 84..112
PFAM efhand: EF hand 120..148
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 5..71
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 81..144
PROSITE EF_HAND: EF-hand calcium-binding domain. 130..142
PROSITE EF_HAND: EF-hand calcium-binding domain. 21..33
PROSITE EF_HAND: EF-hand calcium-binding domain. 57..69
PROSITE EF_HAND: EF-hand calcium-binding domain. 94..106
__________________
Plus Strand HSPs:
Score = 137 (48.2 bits), Expect = 1.8e-08, P = 1.8e-08
Identities = 29/80 (36%), Positives = 49/80 (61%), Frame = +1
Query: 49 FLRVFSKVMR---MNTEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + +K M+ E + FD+DG+G IS ELR+ + +G +L ++ +++I E
Sbjct: 69 FLTMMAKKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIRE 128
Query: 220 LDSDGDGFLSLEDFVKLMEA 279
D DGDG ++ E+FV++M A
Sbjct: 129 ADIDGDGQVNYEEFVQMMTA 148
Score = 117 (41.2 bits), Expect = 2.4e-06, P = 2.4e-06
Identities = 24/81 (29%), Positives = 45/81 (55%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLT 71
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
+M +D + +++ EAF +
Sbjct: 72 MMAKKMKDTDSEEEIREAFRV 92
Score = 79 (27.8 bits), Expect = 0.031, P = 0.031
Identities = 28/80 (35%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E L M+ ++ D+E+ I E D DG+G++S
Sbjct: 48 ELQDMINEVDADGNGTIDFPEF---LTMMAKKMKDTDSEEEIREAFRVFDKDGNGYISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 105 ELRHVMTNLGE--KLTD-EEVDEM 125
>gi|115503|sp|P15094|CALM_ACHKL CALMODULIN >gi|71674|pir||MCUMAK calmodulin -
Achlya klebsiana >gi|166304|gb|AAA32627.1| (J05116) calmodulin
[Achlya klebsiana]
Length = 149
Frame 1 hits (HSPs): ______________________________________________
Annotated Domains: _________________________ ________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
__________________
Annotated Domains:
BLOCKS BL00018: EF-hand calcium-binding domain 129..141
DOMO DM00011: CALMODULINREPEAT 1..32
Entrez methylation site: (BY SIMILARITY). 116
Entrez Calcium binding region: SITE 1. 21..32
Entrez Calcium binding region: SITE 2. 57..68
Entrez Calcium binding region: SITE 3. 94..105
Entrez Calcium binding region: SITE 4. 130..141
PFAM efhand: EF hand 11..39
PFAM efhand: EF hand 47..75
PFAM efhand: EF hand 84..112
PFAM efhand: EF hand 120..148
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 5..71
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 81..144
PROSITE EF_HAND: EF-hand calcium-binding domain. 130..142
PROSITE EF_HAND: EF-hand calcium-binding domain. 21..33
PROSITE EF_HAND: EF-hand calcium-binding domain. 57..69
PROSITE EF_HAND: EF-hand calcium-binding domain. 94..106
__________________
Plus Strand HSPs:
Score = 137 (48.2 bits), Expect = 1.8e-08, P = 1.8e-08
Identities = 29/78 (37%), Positives = 50/78 (64%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFERVLKYF---DEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ M+ E +L+ F D+DG+G IS ELR+ + +G +L ++ +++I E
Sbjct: 69 FLTMMARKMKDTDSEEEILEAFQGFDKDGNGFISAAELRHMMTNLGEKLTDEEVDEMIRE 128
Query: 220 LDSDGDGFLSLEDFVKLM 273
D DGDG ++ E+FVK+M
Sbjct: 129 ADIDGDGQINYEEFVKMM 146
Score = 112 (39.4 bits), Expect = 8.1e-06, P = 8.1e-06
Identities = 24/80 (30%), Positives = 45/80 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAGSLFDKDGDGTITTKELGTVMRSVGQNPTEAELQDMINEVDADGNGTIDFPEFLT 71
Query: 268 LMEAAGED-EKLKDLEEAFE 324
+M +D + +++ EAF+
Sbjct: 72 MMARKMKDTDSEEEILEAFQ 91
Score = 80 (28.2 bits), Expect = 0.024, P = 0.024
Identities = 29/80 (36%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E L M+ ++ D+E+ I E D DG+GF+S
Sbjct: 48 ELQDMINEVDADGNGTIDFPEF---LTMMARKMKDTDSEEEILEAFQGFDKDGNGFISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 105 ELRHMMTNLGE--KLTD-EEVDEM 125
>gi|3800849|gb|AAC68891.1| (AF081670) VU91C calmodulin [synthetic construct]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
Plus Strand HSPs:
Score = 137 (48.2 bits), Expect = 1.8e-08, P = 1.8e-08
Identities = 30/82 (36%), Positives = 53/82 (64%), Frame = +1
Query: 43 SIFLRVFSKVMRMNTEFERVL---KYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLI 213
S FL + S+ ++ N + +L + FD+DG+G IS ELR+ + +G +L ++ +++I
Sbjct: 67 SEFLALMSRQLKSNDSEQELLEAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMI 126
Query: 214 EELDSDGDGFLSLEDFVKLMEA 279
E D DGDG ++ E+FV++M A
Sbjct: 127 READVDGDGQVNYEEFVQVMMA 148
Score = 103 (36.3 bits), Expect = 7.3e-05, P = 7.3e-05
Identities = 24/81 (29%), Positives = 42/81 (51%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D DG+ + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDVDGNHQIEFSEFLA 71
Query: 268 LMEAA-GEDEKLKDLEEAFEM 327
LM ++ ++L EAF +
Sbjct: 72 LMSRQLKSNDSEQELLEAFRV 92
Score = 81 (28.5 bits), Expect = 0.018, P = 0.018
Identities = 28/80 (35%), Positives = 44/80 (55%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEK-LIEEL---DSDGDGFLSLE 255
E + ++ D DG+ +I E L ++ +L + D+E+ L+E D DG+GF+S
Sbjct: 48 ELQDMINEVDVDGNHQI---EFSEFLALMSRQLKSNDSEQELLEAFRVFDKDGNGFISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 105 ELRHVMTNLGE--KLTD-EEVDEM 125
>gi|1168751|sp|P23286|CALM_CANAL CALMODULIN >gi|7597005|gb|AAA34331.2| (M61128)
calmodulin [Candida albicans]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
Annotated Domains: __________________________ _______________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
__________________
Annotated Domains:
BLOCKS BL00018: EF-hand calcium-binding domain 130..142
DOMO DM00011: CALMODULINREPEAT 1..33
Entrez Calcium binding region: SITE 1. 21..32
Entrez Calcium binding region: SITE 2. 57..68
Entrez Calcium binding region: SITE 3. 94..105
Entrez Calcium binding region: SITE 4. 130..141
PFAM efhand: EF hand 12..40
PFAM efhand: EF hand 48..76
PFAM efhand: EF hand 85..113
PFAM efhand: EF hand 121..149
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 6..72
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 82..141
PROSITE EF_HAND: EF-hand calcium-binding domain. 130..142
PROSITE EF_HAND: EF-hand calcium-binding domain. 21..33
PROSITE EF_HAND: EF-hand calcium-binding domain. 57..69
PROSITE EF_HAND: EF-hand calcium-binding domain. 94..106
__________________
Plus Strand HSPs:
Score = 137 (48.2 bits), Expect = 1.8e-08, P = 1.8e-08
Identities = 29/80 (36%), Positives = 47/80 (58%), Frame = +1
Query: 49 FLRVFSKVMR---MNTEFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE 219
FL + ++ M+ E K FD +GDGKIS ELR+ L IG +L D +++I+E
Sbjct: 69 FLTMMARKMKDTDSEAEIAEAFKVFDRNGDGKISAAELRHLLTSIGEKLSDADVDQMIKE 128
Query: 220 LDSDGDGFLSLEDFVKLMEA 279
D++ DG + +++F L+ A
Sbjct: 129 ADTNNDGEIDIQEFTSLLAA 148
Score = 103 (36.3 bits), Expect = 7.3e-05, P = 7.3e-05
Identities = 23/81 (28%), Positives = 42/81 (51%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+D DGKI+ EL + +G + +I E+D + DG + +F+
Sbjct: 12 EFKEAFSLFDKDSDGKITTKELGTVMRSLGQNPSESELTDMINEVDVNSDGSIDFPEFLT 71
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
+M +D + ++ EAF++
Sbjct: 72 MMARKMKDTDSEAEIAEAFKV 92
>gi|3800847|gb|AAC68890.1| (AF081669) VU91B calmodulin [synthetic construct]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
Plus Strand HSPs:
Score = 137 (48.2 bits), Expect = 1.8e-08, P = 1.8e-08
Identities = 30/82 (36%), Positives = 53/82 (64%), Frame = +1
Query: 43 SIFLRVFSKVMRMNTEFERVL---KYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLI 213
S FL + S+ ++ N + +L + FD+DG+G IS ELR+ + +G +L ++ +++I
Sbjct: 67 SEFLALMSRQLKSNDSEQELLEAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMI 126
Query: 214 EELDSDGDGFLSLEDFVKLMEA 279
E D DGDG ++ E+FV++M A
Sbjct: 127 READVDGDGQVNYEEFVQVMMA 148
Score = 94 (33.1 bits), Expect = 0.00065, P = 0.00065
Identities = 23/81 (28%), Positives = 40/81 (49%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+D +G IS EL + +G + L+ E+D DG+ + +F+
Sbjct: 12 EFKEAFALFDKDNNGSISSSELATVMRSLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLA 71
Query: 268 LMEAA-GEDEKLKDLEEAFEM 327
LM ++ ++L EAF +
Sbjct: 72 LMSRQLKSNDSEQELLEAFRV 92
Score = 79 (27.8 bits), Expect = 0.031, P = 0.031
Identities = 28/80 (35%), Positives = 43/80 (53%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEK-LIEEL---DSDGDGFLSLE 255
E ++ D DG+ +I E L ++ +L + D+E+ L+E D DG+GF+S
Sbjct: 48 EVNDLMNEIDVDGNHQI---EFSEFLALMSRQLKSNDSEQELLEAFRVFDKDGNGFISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 105 ELRHVMTNLGE--KLTD-EEVDEM 125
>gi|4959162|gb|AAD34259.1|AF084411_1 (AF084411) calmodulin mutant SYNCAM57A
[synthetic construct]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
Plus Strand HSPs:
Score = 136 (47.9 bits), Expect = 2.3e-08, P = 2.3e-08
Identities = 31/80 (38%), Positives = 53/80 (66%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFERVLK----YFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIE 216
FL + ++ M+ +T+ E LK FD+DG+G IS ELR+ + +G +L ++ +++I
Sbjct: 69 FLNLMARAMK-DTDSEEELKEAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIR 127
Query: 217 ELDSDGDGFLSLEDFVKLMEA 279
E D DGDG ++ E+FV++M A
Sbjct: 128 EADVDGDGQVNYEEFVQVMMA 148
Score = 128 (45.1 bits), Expect = 1.6e-07, P = 1.6e-07
Identities = 27/81 (33%), Positives = 47/81 (58%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLN 71
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
LM A +D + ++L+EAF +
Sbjct: 72 LMARAMKDTDSEEELKEAFRV 92
Score = 78 (27.5 bits), Expect = 0.083, P = 0.080
Identities = 27/80 (33%), Positives = 42/80 (52%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E N ++ + D+E+ ++E D DG+GF+S
Sbjct: 48 ELQDMINEVDADGNGTIDFPEFLN---LMARAMKDTDSEEELKEAFRVFDKDGNGFISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 105 ELRHVMTNLGE--KLTD-EEVDEM 125
>gi|4959618|gb|AAD34422.1|AF084438_1 (AF084438) calmodulin mutant SYNCAM45
[synthetic construct]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
Plus Strand HSPs:
Score = 136 (47.9 bits), Expect = 2.3e-08, P = 2.3e-08
Identities = 31/80 (38%), Positives = 54/80 (67%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFERVLK----YFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIE 216
FL + ++ M+ +T+ E+ LK FD+DG+G IS ELR+ + +G +L ++ +++I
Sbjct: 69 FLNLMARKMK-DTDSEKELKEAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIR 127
Query: 217 ELDSDGDGFLSLEDFVKLMEA 279
E D DGDG ++ E+FV++M A
Sbjct: 128 EADVDGDGQVNYEEFVQVMMA 148
Score = 127 (44.7 bits), Expect = 2.1e-07, P = 2.1e-07
Identities = 27/81 (33%), Positives = 46/81 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLN 71
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
LM +D + K+L+EAF +
Sbjct: 72 LMARKMKDTDSEKELKEAFRV 92
Score = 84 (29.6 bits), Expect = 0.0079, P = 0.0078
Identities = 28/80 (35%), Positives = 43/80 (53%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E N ++ ++ D+EK ++E D DG+GF+S
Sbjct: 48 ELQDMINEVDADGNGTIDFPEFLN---LMARKMKDTDSEKELKEAFRVFDKDGNGFISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 105 ELRHVMTNLGE--KLTD-EEVDEM 125
>gi|4959157|gb|AAD34254.1|AF084406_1 (AF084406) calmodulin mutant SYNCAM53
[synthetic construct]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
Plus Strand HSPs:
Score = 136 (47.9 bits), Expect = 2.3e-08, P = 2.3e-08
Identities = 32/80 (40%), Positives = 52/80 (65%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFERVLK----YFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIE 216
FL + ++ M+ +T+ E LK FD+DG+G IS ELR+ + +G +L K +++I
Sbjct: 69 FLNLMARKMK-DTDSEEELKEAFRVFDKDGNGFISAAELRHVMTNLGEKLTKKKVDEIIR 127
Query: 217 ELDSDGDGFLSLEDFVKLMEA 279
E D DGDG ++ E+FV++M A
Sbjct: 128 EADVDGDGQVNYEEFVQVMMA 148
Score = 126 (44.4 bits), Expect = 2.7e-07, P = 2.7e-07
Identities = 27/81 (33%), Positives = 46/81 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G K + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTKKKLQDMINEVDADGNGTIDFPEFLN 71
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
LM +D + ++L+EAF +
Sbjct: 72 LMARKMKDTDSEEELKEAFRV 92
Score = 70 (24.6 bits), Expect = 4.8, P = 0.99
Identities = 21/77 (27%), Positives = 40/77 (51%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
+ + ++ D DG+G I E N ++ ++ D+E+ ++E D DG+GF+S
Sbjct: 48 KLQDMINEVDADGNGTIDFPEFLN---LMARKMKDTDSEEELKEAFRVFDKDGNGFISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEE 315
+ +M GE K ++E
Sbjct: 105 ELRHVMTNLGEKLTKKKVDE 124
>gi|4959151|gb|AAD34248.1|AF084400_1 (AF084400) calmodulin mutant SYNCAM48
[synthetic construct]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
Plus Strand HSPs:
Score = 136 (47.9 bits), Expect = 2.3e-08, P = 2.3e-08
Identities = 31/80 (38%), Positives = 54/80 (67%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFERVLK----YFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIE 216
FL + ++ M+ +T+ E+ LK FD+DG+G IS ELR+ + +G +L ++ +++I
Sbjct: 69 FLNLMARKMK-DTDSEKKLKEAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIR 127
Query: 217 ELDSDGDGFLSLEDFVKLMEA 279
E D DGDG ++ E+FV++M A
Sbjct: 128 EADVDGDGQVNYEEFVQVMMA 148
Score = 124 (43.7 bits), Expect = 4.3e-07, P = 4.3e-07
Identities = 27/81 (33%), Positives = 45/81 (55%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLN 71
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
LM +D + K L+EAF +
Sbjct: 72 LMARKMKDTDSEKKLKEAFRV 92
Score = 85 (29.9 bits), Expect = 0.0059, P = 0.0058
Identities = 28/80 (35%), Positives = 43/80 (53%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E N ++ ++ D+EK ++E D DG+GF+S
Sbjct: 48 ELQDMINEVDADGNGTIDFPEFLN---LMARKMKDTDSEKKLKEAFRVFDKDGNGFISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 105 ELRHVMTNLGE--KLTD-EEVDEM 125
>gi|4959172|gb|AAD34269.1|AF084421_1 (AF084421) calmodulin mutant SYNCAM71A
[synthetic construct]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
Plus Strand HSPs:
Score = 136 (47.9 bits), Expect = 2.3e-08, P = 2.3e-08
Identities = 31/80 (38%), Positives = 53/80 (66%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFERVLK----YFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIE 216
FL + ++ M+ +T+ E LK FD+DG+G IS ELR+ + +G +L ++ +++I
Sbjct: 69 FLNLMARKMK-DTDSEEELKEAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIR 127
Query: 217 ELDSDGDGFLSLEDFVKLMEA 279
E D DGDG ++ E+FV++M A
Sbjct: 128 EADVDGDGQVNYEEFVQVMSA 148
Score = 123 (43.3 bits), Expect = 5.5e-07, P = 5.5e-07
Identities = 26/81 (32%), Positives = 46/81 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLN 71
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
LM +D + ++L+EAF +
Sbjct: 72 LMARKMKDTDSEEELKEAFRV 92
Score = 80 (28.2 bits), Expect = 0.024, P = 0.024
Identities = 27/80 (33%), Positives = 43/80 (53%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E N ++ ++ D+E+ ++E D DG+GF+S
Sbjct: 48 ELQDMINEVDADGNGTIDFPEFLN---LMARKMKDTDSEEELKEAFRVFDKDGNGFISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEEAFEM 327
+ +M GE KL D EE EM
Sbjct: 105 ELRHVMTNLGE--KLTD-EEVDEM 125
>gi|4959154|gb|AAD34251.1|AF084403_1 (AF084403) calmodulin mutant SYNCAM51
[synthetic construct]
Length = 149
Frame 1 hits (HSPs): _______________________________________________
__________________________________________________
Database sequence: | | | | 149
0 50 100
Plus Strand HSPs:
Score = 136 (47.9 bits), Expect = 2.3e-08, P = 2.3e-08
Identities = 32/80 (40%), Positives = 52/80 (65%), Frame = +1
Query: 49 FLRVFSKVMRMNTEFERVLK----YFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIE 216
FL + ++ M+ +T+ E LK FD+DG+G IS ELR+ + +G +L K +++I
Sbjct: 69 FLNLMARKMK-DTDSEEELKEAFRVFDKDGNGFISAAELRHVMTNLGEKLTKKKVDEIIR 127
Query: 217 ELDSDGDGFLSLEDFVKLMEA 279
E D DGDG ++ E+FV++M A
Sbjct: 128 EADVDGDGQVNYEEFVQVMMA 148
Score = 123 (43.3 bits), Expect = 5.5e-07, P = 5.5e-07
Identities = 26/81 (32%), Positives = 46/81 (56%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEELDSDGDGFLSLEDFVK 267
EF+ FD+DGDG I+ EL + +G + + +I E+D+DG+G + +F+
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLN 71
Query: 268 LMEAAGED-EKLKDLEEAFEM 327
LM +D + ++L+EAF +
Sbjct: 72 LMARKMKDTDSEEELKEAFRV 92
Score = 74 (26.0 bits), Expect = 0.87, P = 0.58
Identities = 22/77 (28%), Positives = 40/77 (51%), Frame = +1
Query: 88 EFERVLKYFDEDGDGKISPCELRNRLGMIGGELLAKDAEKLIEE----LDSDGDGFLSLE 255
E + ++ D DG+G I E N ++ ++ D+E+ ++E D DG+GF+S
Sbjct: 48 ELQDMINEVDADGNGTIDFPEFLN---LMARKMKDTDSEEELKEAFRVFDKDGNGFISAA 104
Query: 256 DFVKLMEAAGEDEKLKDLEE 315
+ +M GE K ++E
Sbjct: 105 ELRHVMTNLGEKLTKKKVDE 124
WARNING: HSPs involving 941 database sequences were not reported due to the
limiting value of parameter B = 50.
Parameters:
filter=none
matrix=BLOSUM62
V=50
B=50
E=10
gi
H=1
sort_by_pvalue
echofilter
ctxfactor=5.90
Query ----- As Used ----- ----- Computed ----
Frame MatID Matrix name Lambda K H Lambda K H
Std. 0 BLOSUM62 0.318 0.135 0.401
+3 0 BLOSUM62 0.318 0.135 0.401 0.383 0.177 0.878
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
+2 0 BLOSUM62 0.318 0.135 0.401 0.376 0.166 0.707
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
+1 0 BLOSUM62 0.318 0.135 0.401 0.326 0.146 0.409
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
-1 0 BLOSUM62 0.318 0.135 0.401 0.340 0.142 0.489
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
-2 0 BLOSUM62 0.318 0.135 0.401 0.355 0.159 0.552
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
-3 0 BLOSUM62 0.307 0.121 0.319 same same same
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
Query
Frame MatID Length Eff.Length E S W T X E2 S2
+3 0 108 108 10. 68 3 12 22 0.12 32
29 0.12 34
+2 0 109 108 10. 68 3 12 22 0.12 32
29 0.12 34
+1 0 109 108 10. 68 3 12 22 0.12 32
29 0.12 34
-1 0 109 108 10. 68 3 12 22 0.12 32
29 0.12 34
-2 0 109 108 10. 68 3 12 22 0.12 32
29 0.12 34
-3 0 108 108 10. 68 3 12 23 0.098 33
29 0.12 34
Statistics:
Database: /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
Title: nr
Release date: unknown
Posted date: 8:50 PM CDT May 27, 2000
Format: BLAST
# of letters in database: 158,518,215
# of sequences in database: 505,245
# of database sequences satisfying E: 991
No. of states in DFA: 589 (58 KB)
Total size of DFA: 151 KB (192 KB)
Time to generate neighborhood: 0.01u 0.00s 0.01t Elapsed: 00:00:00
No. of threads or processors used: 4
Search cpu time: 160.64u 1.09s 161.73t Elapsed: 00:01:03
Total cpu time: 160.77u 1.13s 161.90t Elapsed: 00:01:03
Start: Wed Feb 14 22:18:39 2001 End: Wed Feb 14 22:19:42 2001
WARNINGS ISSUED: 2
Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000