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Title Immunodissection and culture of rabbit cortical collecting tubule cells
Creator/Author Spielman, W.S. ; Sonnenburg, W.K. ; Allen, M.L. ; Arend, L.J. ; Gerozissis, K. ; Smith, W.L.
Publication Date1986 Aug 01
OSTI IdentifierOSTI ID: 6295713
Other Number(s)CODEN: AJPHA
Resource TypeJournal Article
Resource RelationAm. J. Physiol. ; Vol/Issue: 251:2
Research OrgMichigan State Univ., East Lansing
Subject550301 -- Cytology-- Tracer Techniques; AMP-- BIOSYNTHESIS;LABELLED COMPOUNDS-- BIOSYNTHESIS;TUBULES-- BIOCHEMISTRY;TUBULES-- MORPHOLOGY; ANIMAL CELLS;ANTIGENS;CELL CULTURES;IMMUNOGLOBULINS;IODINE 125;MONOCLONAL ANTIBODIES;PARATHORMONE;PROSTAGLANDINS;RABBITS;TRITIUM COMPOUNDS;VASOPRESSIN
Related SubjectANIMALS;ANTIBODIES;BETA DECAY RADIOISOTOPES;BODY;CHEMISTRY;DAYS LIVING RADIOISOTOPES;ELECTRON CAPTURE RADIOISOTOPES;GLOBULINS;HORMONES;INTERMEDIATE MASS NUCLEI;IODINE ISOTOPES;ISOTOPES;KIDNEYS;LABELLED COMPOUNDS;MAMMALS;NUCLEI;NUCLEOTIDES;ODD-EVEN NUCLEI;ORGANIC COMPOUNDS;ORGANS;PEPTIDE HORMONES;PITUITARY HORMONES;PROTEINS;RADIOISOTOPES;SYNTHESIS;VERTEBRATES
Description/Abstract A mouse monoclonal antibody designated IgG3 (rct-30) has been prepared that reacts specifically with an antigen on the surface of all cells comprising the cortical and medullary rabbit renal collecting tubule including the arcades.^Plastic culture dishes coated with IgG3 (rct-30) were used to isolate collecting tubule cells from collagenase dispersions of rabbit renal cortical cells by immunoadsorption.^Typically, 10W rabbit cortical collecting tubule (RCCT) cells were obtained from 5 g of renal cortex (2 kidneys).^Between 20 and 30% of the RCCT cells were reactive with peanut lectin suggesting that RCCT cells are a mixture of principal and intercalated cells.^Approximately 10X RCCT cells were obtained after 4 to 5 days in primary culture.^Moreover, RCCT cells continued to proliferate after passaging with a doubling time of approx.32 h. RCCT cells passaged once and then cultured 4-5 days were found 1) to synthesize cAMP in response to arginine vasopressin (AVP), prostaglandin E2 (PGE2), isoproterenol, and parathyroid hormone, but not calcitonin, prostaglandin D2, or prostaglandin I, and 2) to release PGE2 in response to bradykinin but not arginine vasopressin or isoproterenol.^The results indicate that cultured RCCT cells retain many of the hormonal, histochemical, and morphological properties expected for a mixture of principal and intercalated rabbit cortical collecting tubule epithelia.^RCCT cells should prove useful both for studying hormonal interactions in the cortical collecting tubule and as a starting population for isolating intercalated collecting tubule epithelia.
Country of PublicationUnited States
LanguageEnglish
FormatPages: F348-F357
System Entry Date2001 May 13

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