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Title Senescence of nickel-transformed cells by an X chromosome: Possible epigenetic control
Creator/Author Klein, C.B. ; Xin Wei Wang ; Bhamra, R.K. ; Xinhua Lin ; Cohen, M.D. ; Costa, M. (New York Univ. Medical Center, NY (United States)) ; Conway, K. (Univ. of North Carolina Medical School, Chapel Hill (United States)) ; Annab, L. ; Barrett, J.C. (National Inst. of Environmental Health Sciences, Research Triangle Park, NC (United States))
Publication Date1991 Feb 15
OSTI IdentifierOSTI ID: 5596855
Other Number(s)ISSN0036-8075; CODEN: SCIEA
Resource TypeJournal Article
Resource RelationScience (Washington, D.C.) ; Vol/Issue: 251:4995
Subject560300 -- Chemicals Metabolism & Toxicology; ANIMAL CELLS-- ONCOGENIC TRANSFORMATIONS;DNA-- METHYLATION;NICKEL-- CARCINOGENESIS; GENE REGULATION;GENES;HAMSTERS;METABOLISM;X CHROMOSOME
Related SubjectANIMALS;CELL TRANSFORMATIONS;CHEMICAL REACTIONS;CHROMOSOMES;ELEMENTS;HETEROCHROMOSOMES;MAMMALS;METALS;NUCLEIC ACIDS;ORGANIC COMPOUNDS;PATHOGENESIS;RODENTS;TRANSITION ELEMENTS;VERTEBRATES
Description/Abstract Transfer of a normal Chinese hamster X chromosome (carried in a mouse A9 donor cell line) to a nickel-transformed Chinese hamster cell line with an Xq chromosome deletion resulted in senescense of these previously immortal cells.^At early passages of the A9/CX donor cells, the hamster X chromosome was highly active, inducing senescence in 100% of the colonies obtained after its transfer into the nickel-transformed cells.^However, senescence was reduced to 50% when Chinese hamster X chromosomes were transferred from later passage A9 cells.^Full senescing activity of the intact hamster X chromosome was restored by treatment of the donor mouse cells with 5-azacytidine, which induced demethylation of DMA.^These results suggest that a senescence gene or genes, which may be located on the Chinese hamster X chromosome, can be regulated by DNA methylation, and that escape from senescence and possibly loss of tumor suppressor gene activity can occur by epigenetic mechanisms.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 796-799
System Entry Date2001 May 13

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