Record 333 of 382 - CRIS/ICAR 9/96
TI: REGULATION OF GENE EXPRESSION BY ETHYLENE
IN: ABELES-F-B
PO: FRUIT CULTURE RESEARCH APPALACHIAN FRUIT RESEARCH LAB. AGRICULTURAL
RESEARCH SERVICE
LO: KEARNEYSVILLE, WEST VIRGINIA 25430.
FY: 1990
CL:
PRIMARY CLASSIFICATION GENERAL CLASSIF.
RPA ACTVTY CMMDTY SCNCE PRCNT PRGM JTC
R404 A5800 C1000 F1312 100% P3.13 J3A
PH: R404 (Quality-Maintenance-Fruit,-Vegetables);
A5800 (Measure-and-Maintain-Market-Quality);
C1000 (Deciduous-and-Small-Fruits-and-Nuts);
F1312 (Physiology-Plant)
GH: P3.13 (Fruit);
J3A (Food-Systems)
SC: S1015 (Peaches) 100%
DE: ETHYLENE; FRUIT; RIPENING; DNA; ENZYMES; GENE-REGULATION; PLANT-GENETICS;
GENES; GENE-EXPRESSION; SENESCENCE; RABBITS; ANTIBODY-PRODUCTION;
DNA-STRUCTURE; PLANT-PHYSIOLOGY; GLUCANASES
CT: Basic 90%; Applied 10%; Developmental 0%
OB: Determine the effect of ethylene on the induction of beta-1,3-glucanase;
elucidate the role which this enzyme has in senescence of fruit.
AP: Purify to homogeneity beta-1,3-glucanase and make antibodies to it in
rabbits. Identify the cDNA coding for the enzyme.
PR: 8901 TO 8912
The role of cellulase in fruit ripening was twofold. The enzyme appears
to control both the expansion growth of fruit such as apple and strawberry and
softening in blackberry and strawberry. The enzymatic basis of softening in
apple is still unknown. While polygalacturonase is important in the softening
of fruits such as tomato, peach, avocado and pear, it does not function in
loss of flesh firmness in apple, strawberry, and blackberry. Peroxidase in
members of the Cucurbitaceae was evaluated because it represents a major gene
product in ethylene treated plants. Two isoenzymes, acidic and basic, were
purified and antibodies used in quantification and immunocytochemical
localization. The basic peroxidase undergoes 25-fold induction during aging or
ethylene action and was localized around starch grains. The acid isoenzyme was
increased 3-fold and localized in both the cytoplasm and vacuole. We evaluated
the various theories suggesting that these enzymes play a role in chlorophyll
degradation, disease resistance or lignin formation. No conclusive evidence
was obtained that any of the above described the true function of these
proteins. Peroxidase was cloned and sequenced and shown to be similar to other
plant heme proteins. A restriction fragment length polymorphism analysis of
DNA integrity during senescence has indicated that senescence in plants is not
due to DNA breakdown but to the synthesis of specific hydrolases encoded by
the DNA.
PB: 8901 TO 8912
ABELES, F.B., HERSHBERGER, W.L., DUNN, L.J. 1989. Hormonal regulation and
intracellular localization of a 33-kD cationic peroxidase in excised cucumber
cotyledons. Plant Physiol. 89:664-668. SAN ANTONIO, J.P., ABELES, F.B., and
HANNERS, P.K. 1989. Shiitake mushroom production on oak logs inoculated with
grain-spawn disks by 23 different shiitaki strains. Mushroom J for the Tropics
9:161-164. ABELES, F.B. and DUNN, L.J. 1989. Role of peroxidase during
ethylene-induced chlorophyll breakdown in Cucumis sativus cotyledons. J. Plant
Growth Regulation 8:319-325. ABELES, F.B. and TAKEDA, F. 1989. Increased
cellulase activity during blackberryfruit ripening. HortScience 24:851.
ABELES, F.B., BILES, C.L. and DUNN, L.J. 1989. Induction of peroxidase as a
response to environmental stimuli. In: Mechanism of plant perception response
toenvironmental Stimulus. Mono. 20, British Plant Reg. Group.
PB: 8801 TO 8812
ABELES, F.B., DUNN, L.J., MORGENS, P., CALLAHAN, A., DINTERMAN, R.E.,
SCHMIDT,J 1988. Induction of 33-kDa and 60-kD peroxidases during
ethylene-induced senescence of cucumber cotyledons. Plant Physiol. 87:609-615.
PB: 8701 TO 8712
ABELES, F.B. 1987. Plant chemiluminescence: An overview. Physiol. Plant.
71:127-130. ABELES, F.B. and WYDOSKI, S.G. 1987. Inhibitors of ethylene
synthetics and action: a companion of their activities in a lettuce root
growth model system. J. Amer. Soc. Hort. Sci. 112:122-125. ABELES, F.B. 1987.
Manipulation of plant growth by ethylene. Acta Horticulturae 201:11-20.
PB: 8601 TO 8612
ABELES, F.B. 1986. Role of ethylene in lactuca sativa cv 'Grand Rapids'
seed germination. Plant Physiol. 81:780-787. ABELES, F.B. 1986. Plant
chemiluminescence. Ann. Rev. Plant Physiol. 37:49-72. MORGENS, P.H., PYLE,
J.B. AND CALLAHAN, A.M. 1986. Searching for molecular mechanisms involved in
fruit ripening. UCLA Symposium on Molecular and Cellular Biology, New Series,
Vol. 44. J.E. Fox & M. Jacobs, Eds. NY.
PB: 8501 TO 8512
Abeles, F.B., L.J. Dunn. 1985. Ethylene-enhanced ethylene oxidation in
vicia faba. J. Plant Growth Regulation 4:123-128. Abeles, F.B. 1985. Ethylene
and plant development: An introduction In Ethylene and Plant Development, Eds.
Roberts, J.A., and G.A. Tucker pp 1-8. Abeles, F.B. 1985. Sources of ethylene
of horticultural significance. In Ethylene and Plant Development, Eds.
Roberts, J.A., and G.A. Tucker, pp. 287-296. Abeles, F.B. 1986. Plant
chemiluminescence. An. Rev. Plant Physiol. In press.
OC: 007230
IC: 008588
SU: Region 1 (Northeastern).
PS: NEW
UD: 8706
UP: 9003
PP: 8912
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