Version 2.5.2.0

Abstract

Grant Number:	1F32AG027631-01
Project Title:	Delineating the role of Rb in cellular senescence

PI Information:	Name	Email	Title
	CHICAS, AGUSTIN	chicas@cshl.edu

Abstract: DESCRIPTION (provided by applicant): The goal of this proposal is to dissect the role of the Rb family of proteins in cellular senescence, identify the targets of Rb during cellular senescence and identify genes that that contribute to the stability of senescence. Because components of the senescence machinery have been shown to play important roles in aging and cancer, this approach could lead to the identification of new molecules with potential role in ageing and/or cancer. These questions will be addressed using the well characterize IMR90 human diploid fibroblast. These cells can be induced to undergo senescence by exogenous expression of oncogenic ras, etoposide, enforce expression of p16, or extensive passage. Senescent IMR90 cells display the characteristic associated with senescence such as irreversible cell cycle arrest, large flat morphology, senescence-associated beta-galactosidase (SA-beta-gal) activity and heterochromatic foci. These characteristics will be used together with RNAi technology to assess the role of the different Rb family members in senescence and to identify factors that contribute to the stability of senescence. RNAi technology will also be combined with expression microarray to identify the targets of Rb during senescence.

Public Health Relevance:
This Public Health Relevance is not available.

Thesaurus Terms:
cell senescence, retinoblastoma protein, tumor suppressor gene
cell cycle, cell growth regulation, cell proliferation, gene induction /repression, heterochromatin, transcription factor
RNA interference, cell line, cell morphology, chromatin immunoprecipitation, human genetic material tag, immunofluorescence technique, microarray technology, postdoctoral investigator, transfection

Institution:	COLD SPRING HARBOR LABORATORY
	P.O. BOX 100
	COLD SPRING HARBOR, NY 11724
Fiscal Year:	2006
Department:
Project Start:	01-APR-2006
Project End:	31-MAR-2009
ICD:	NATIONAL INSTITUTE ON AGING
IRG:	ZRG1

---