Kuijper EJ, De Wever B, Snijders F, Danner SA, Dankert J; American Society for Microbiology. General Meeting.

Abstr Gen Meet Am Soc Microbiol. 1998 May 17-21; 98: 173 (abstract no. C-254).

Department of Medical Microbiology, Univer. of Amsterdam, The Netherlands.

A cross-sectional study was performed at the outpatients AIDS department to assess the prevalence of Campylobacter species in stool specimens from 201 consecutive HIV-seropositive patients. We performed cultures with use of a membrane filter technique on nonselective bloodagar and characterized campylobacters phenotypically and genetically. Primers used were based on 23S rDNA sequences for the identification of the group of thermophilic species (C. jejuni, C. coli, C. lari and C.upsaliensis) and for the individual species C. hyointestinalis, C. fetus, C. mucosalis, C.concisus and C.sputorum. Thermophilic species were further identified with a recent described technique of RFLP-PCR on a putative haem-copper oxidase gene. Campylobacter species were the most frequent enteropathogenic bacteria from 7 (16%) of 43 patients with diarrhea and 5 (3%) of 158 patients without diarrhea (p = 0.001). of 10 strains available for phenotyical and genetical identification, only 3 were correctly identified phenotypically (1 C. upsaliensis, 1 C. jejuni subsp. jejuni, 1 C. jejuni subsp. doylei). Genetical characterization resulted in 3 C. lari, 2 C. jejuni, 2 C. upsaliensis, and 1 C. coli. the remaining two strains did not react with the available primers but 23S rDNA sequencing confirmed their identification as campylobacters. of 12 campylobacter strains, 2 C. lari were resistant to erythromycin. PCR for thermophilic species was also applied directy onto colony sweeps obtained from faecal cultures using the membrane filter method and nonselective bloodagar plates. Six positive faecal cultures from which a sweep was available were also positive by PCR. of 22 HIV-seropositive patients with negative cultures for campylobacters, PCR was positive in 2 of 12 stool cultures of patients with diarrhea and in 2 of 10 cultures of patients without diarrhea. We conclude that campylobacters are an important cause of diarrhea in HIV-infected patients and that genetical identification is more reliable than phenotypical tests. Diagnozing campylobacteriosis by PCR of colony sweeps from nonselective plates after membrane filtration of faecal samples is more sensitive than culture only.

Publication Types:

• Meeting Abstracts

Keywords:

• Acquired Immunodeficiency Syndrome
• Base Sequence
• Campylobacter
• Campylobacter Infections
• Chromosomes
• Cross-Sectional Studies
• Diarrhea
• Dysentery
• Evaluation Studies
• Feces
• HIV Infections
• HIV Seropositivity
• Humans
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length
• Prevalence
• diagnosis
• genetics

Other ID:

• 98296499

UI: 102226451

From Meeting Abstracts