Morey-Holton ER, Tanner SJ, Bikle DD, Doty SB, Halloran BP.
ASGSB Bull. 1993 Oct; 7: 48.
NASA-Ames Research Center, Moffett Field, CA.
Inhibition of periosteal cortical bone formation occurs during skeletal unloading in growing rats. To determine if alendronate (AHBP), a bisphosphonate that inhibits bone resorption, would protect against this skeletal defect, saline or 0.1mg P/kg body weight AHBP was administered on two consecutive days before unloading the hindquarters of young rats. Injections of calcein or demeclocycline were given 6 d before and 1 d after unloading, respectively, to mark bone forming surfaces. Six rats/group (AHBP or saline) were unloaded by tail traction and six rats/group served as controls. After 9 d unloading, tibiae were removed, processed, and sectioned at the tibiofibular junction. Periosteal mineralization was analyzed using the NIH image program. All groups formed bone at about the same rate prior to unloading (approximately 0.08mm2/d). After 9 d unloading, AHBP rats, loaded and unloaded, formed significantly less bone than the control saline rats (15% and 47%, respectively); mineralization rate in saline unloaded animals was similar to AHBP unloaded rats. No change in medullary area was found between groups. The data suggest that AHBP does not protect against suppression of periosteal bone formation during unloading, that the drug, per se, induces suppression of formation, and that endosteal parameters are unaffected by drug treatment or suspension at this sampling site in cortical bone.
Publication Types:
Keywords:
- Alendronate
- Animals
- Bone Resorption
- Bone and Bones
- Diphosphonates
- Hindlimb Suspension
- Naphthalenes
- Osteogenesis
- Rats
- Rats, Inbred Strains
- Rats, Sprague-Dawley
- Tibia
- protect
- NASA Center ARC
- NASA Discipline Musculoskeletal
- NASA Discipline Number 00-00
- NASA Discipline Number 40-40
- NASA Program Flight
- NASA Program Space Biology
- Non-NASA Center
Other ID:
UI: 102212755
From Meeting Abstracts