Name: Ryan J. Case

Mentor: Lane Sander

Division: Analytical Chemistry

Laboratory: Chemical Sciences and Technology Laboratory

Room: A119

Building address: Building 227

Mail Stop: 8392

Telephone: (301) 975-4134

Fax:  (301) 977-0685

Email: ryan.case@nist.gov

Sigma Xi membership?  No

Category: Chemistry

Determination of Flavanols and Organic Acids in Cranberry SRM Development

Ryan J. Case, Katherine E. Sharpless, Stephen A. Wise, Lane C. Sander

As part of a large, collaborative effort between NIST, the Office of Dietary Supplements (ODS) at NIH and the Center for Drug Evaluation and Research (CDER) of the FDA, Standard Reference Materials for popular dietary supplements are being developed for use in the development of new analytical methods, as quality controls and for the support of future clinical trails.  Cranberry (Vaccinium macrocarpon Ait.) has recently become highly popular as a dietary supplement, due to its anti-oxidant activity and anti-adhesion effects on E. coli based urinary-tract infections.  As a result, new Standard Reference Materials of several different product forms of cranberry (juice, powder, extract and tablet) are being developed.  Currently, methods have been developed to analyze and quantify the levels of two flavanols, quercetin and myricetin, and several organic acids, quinic, malic, shikimic, ascorbic and citric acid.  Additionally, several different extraction and hydrolysis techniques were evaluated to identify the most exhaustive techniques in yielding the analytes of interest.