RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Red Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lutjanus campechanus
FDA Seafood List Common Name: Red Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19930816gel02
Unique Web Code: rsh01Srr
Tissue Aliquot Code: lutjcamp 002 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Co-Migrated	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 1
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 1
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	86	10.2	0.0971431	4	0.754515	7.1	5.5	4.55	85.9	4.5	0.0947035
2	5.2	180	21.4	0.187561	3	1.09807	10.3	8	5.2	179.7	3.4	0.184292
3	5.85	283	33.7	0.1902	4	1.2022	11.2	8.8	5.85	283.4	3.5	0.191913
4	6.55	417	49.6	0.151605	6	1.4405	13.5	10.5	6.55	416.2	5.8	0.140333
5	6.85	447	53.2	0.0307788	7	0.336827	3.1	2.5	6.85	447.5	7.1	0.0266369
6	7.35	512	60.9	0.0794942	4	0.532668	5	3.9	7.35	512.1	3.8	0.0785908
7	8.15	646	76.8	0.113591	6	1.27544	11.9	9.3	8.15	645.7	6.4	0.111606
8	8.45	717	85.3	0.139191	5	1.21833	11.4	8.9	8.45	716.6	4.9	0.139177
9	8.65	766	91.1	0.0835306	4	0.623757	5.8	4.6	8.65	765.5	4.2	0.0835899
10	9.3	820	97.5	0.224527	6	2.2182	20.7	16.2	9.3	818.8	5.8	0.216234

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.084	24	2.8	0.024343	5	0.216277	0.6	0.6	4.084	23.1	5	0.0243682
2	4.303	55	6.5	0.296199	5	2.72069	8.2	7.8	4.303	55	5.2	0.296115
3	4.55	90	10.7	0.0951872	3	0.575029	1.7	1.6	4.55	89.9	3.5	0.0939976
4	4.847	132	15.6	0.0304569	5	0.258594	0.8	0.7	4.847	131.5	4.8	0.0302131
5	5.023	157	18.6	0.164809	6	1.69527	5.1	4.9	5.023	157.7	6.4	0.148997
6	5.2	182	21.6	0.107421	6	1.02669	3.1	2.9	5.2	182	6.3	0.0923162
7	5.335	204	24.2	0.232687	3	1.14375	3.4	3.3	5.335	204.6	2.8	0.231204
8	5.451	223	26.4	0.0089568	3	0.0456083	0.1	0.1	5.451	223	3	0.0085542
9	5.574	243	28.8	0.0325117	12	0.748764	2.2	2.1	5.574	248.2	11.8	0.0359283
10	5.672	259	30.7	0.0964126	5	0.771195	2.3	2.2	5.672	259.2	5.4	0.0805902
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.85	288	34.1	0.118762	4	0.843562	2.5	2.4	5.85	288.3	4	0.120275
12	6.017	319	37.8	0.482254	3	2.59954	7.8	7.5	6.017	319.5	3.1	0.477415
13	6.109	336	39.8	0.228693	3	1.29936	3.9	3.7	6.109	336.2	3.3	0.224105
14	6.212	355	42.1	0.218888	5	1.86068	5.6	5.3	6.212	353.7	4.7	0.223268
15	6.298	371	44	0.047939	5	0.416143	1.2	1.2	6.298	370.1	4.7	0.0499404
16	6.379	386	45.7	0.0701315	3	0.385701	1.2	1.1	6.379	386	2.9	0.0745275
17	6.444	398	47.2	0.0422922	5	0.428942	1.3	1.2	6.444	401.4	5.4	0.044595
18	6.514	411	48.7	0.140057	3	0.622833	1.9	1.8	6.514	411	2.5	0.139295
19	6.675	430	50.9	0.507243	4	3.22906	9.7	9.3	6.675	430.3	3.7	0.486859
20	6.806	443	52.5	0.367284	2	1.47496	4.4	4.2	6.806	442.9	2.3	0.366498
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.92	457	54.1	0.084678	4	0.678943	2	1.9	6.92	456.5	4.5	0.0854671
22	7.292	508	60.2	0.192671	3	1.12444	3.4	3.2	7.292	508.2	3.3	0.192423
23	7.35	516	61.1	0.0535747	3	0.33944	1	1	7.35	516.4	3.3	0.0573306
24	7.457	534	63.3	0.0174822	7	0.230313	0.7	0.7	7.457	533.2	6.6	0.0197982
25	7.717	578	68.5	0.0135697	3	0.0833979	0.2	0.2	7.717	577.4	3.5	0.0134728
26	7.8	592	70.1	0.0913201	7	0.932194	2.8	2.7	7.8	595.1	6.5	0.0805915
27	7.854	601	71.2	0.350599	6	3.34012	10	9.6	7.854	600.3	6	0.314254
28	7.954	618	73.2	0.185556	7	1.82775	5.5	5.2	7.954	618	6.5	0.157884
29	8.15	651	77.1	0.0315488	11	0.594774	1.8	1.7	8.15	649.1	11.1	0.030126
30	8.45	720	85.3	0.0569631	5	0.482567	1.4	1.4	8.45	719.7	4.7	0.0573414
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.65	769	91.1	0.051575	4	0.354522	1.1	1	8.65	767.9	4	0.0506349
32	9.3	820	97.2	0.144881	4	1.00929	3	2.9	9.3	819.7	3.8	0.148841

PeakCounter: 32

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.087	22	2.6	0.0403797	6	0.407273	1.1	1	4.087	22.3	5.8	0.0398271
2	4.315	55	6.5	0.347013	6	3.51829	9.6	8.4	4.315	55	5.7	0.346693
3	4.861	134	15.9	0.0469757	5	0.443668	1.2	1.1	4.861	132.9	5.3	0.0475998
4	5.041	160	19	0.280876	6	2.81946	7.7	6.7	5.041	161	6.4	0.24918
5	5.368	210	24.9	0.323712	3	1.64946	4.5	3.9	5.368	210	2.9	0.317737
6	5.486	229	27.1	0.0279692	5	0.226484	0.6	0.5	5.486	229.6	5.5	0.0233092
7	5.611	249	29.5	0.0647735	13	1.37872	3.8	3.3	5.611	251.2	12.6	0.0616685
8	5.71	265	31.4	0.12167	5	0.94413	2.6	2.2	5.71	264.8	5.2	0.102881
9	5.879	293	34.7	0.0252947	3	0.133322	0.4	0.3	5.879	291.9	2.8	0.0270146
10	5.917	300	35.5	0.0294809	4	0.199281	0.5	0.5	5.917	299.7	3.9	0.0286423
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.024	320	37.9	0.546373	3	2.98692	8.2	7.1	6.024	319.9	3.2	0.534899
12	6.109	336	39.8	0.311712	4	2.093	5.7	5	6.109	336.1	4.1	0.285102
13	6.206	354	41.9	0.230838	5	2.16146	5.9	5.1	6.206	353	5.3	0.228458
14	6.281	368	43.6	0.0461452	4	0.307644	0.8	0.7	6.281	369.6	3.7	0.0469541
15	6.372	385	45.6	0.113111	3	0.68352	1.9	1.6	6.372	385.1	3.3	0.11721
16	6.5	409	48.5	0.191552	3	1.02041	2.8	2.4	6.5	408.7	3.3	0.174703
17	6.659	429	50.8	0.563247	4	3.92677	10.7	9.3	6.659	428.9	4.1	0.536953
18	6.782	441	52.3	0.419633	2	1.85295	5.1	4.4	6.782	441.6	2.5	0.41818
19	6.897	454	53.8	0.153428	5	1.27176	3.5	3	6.897	454.1	4.7	0.15409
20	7.007	469	55.6	0.028158	3	0.146366	0.4	0.3	7.007	469.1	2.9	0.0288396
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.096	481	57	0.0172103	2	0.0692821	0.2	0.2	7.096	481.5	2.2	0.0176412
22	7.177	492	58.3	0.0249442	3	0.124135	0.3	0.3	7.177	492.8	3	0.0236889
23	7.287	507	60.1	0.278825	3	1.46812	4	3.5	7.287	506.5	3.1	0.271141
24	7.462	534	63.3	0.0042633	1	0	0	0	7.462	529	1	0
25	7.717	576	68.2	0.0309294	4	0.203418	0.6	0.5	7.717	575.5	3.8	0.03044
26	7.795	589	69.8	0.0284427	4	0.221321	0.6	0.5	7.795	589.4	4.3	0.0292296
27	7.911	608	72	0.338629	11	6.39058	17.4	15.2	7.911	610.8	11.3	0.320491

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	88	10.4	0.0860985	6	0.835056	7.6	6	4.55	88	5.8	0.0810377
2	5.2	184	21.7	0.138219	4	1.04438	9.6	7.5	5.2	184.7	4.4	0.133952
3	5.85	287	33.9	0.215357	3	1.29557	11.8	9.3	5.85	287	3.4	0.213513
4	6.55	419	49.5	0.169847	5	1.49024	13.6	10.7	6.55	418.5	5.4	0.15544
5	6.85	448	53	0.0313978	4	0.198439	1.8	1.4	6.85	447.8	3.6	0.0312895
6	7.35	515	60.9	0.0955479	4	0.663307	6.1	4.8	7.35	515.2	4	0.0930442
7	8.15	644	76.1	0.117002	6	1.3039	11.9	9.4	8.15	643.2	6.3	0.117285
8	8.45	711	84	0.121773	5	1.14201	10.4	8.2	8.45	710.5	5.3	0.121029
9	8.65	760	89.8	0.0685799	5	0.565564	5.2	4.1	8.65	760.5	4.8	0.06691
10	9.3	818	96.7	0.261407	5	2.39739	21.9	17.3	9.3	817.2	5.4	0.250615

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.085	19	2.2	0.0160932	10	0.236636	0.7	0.6	4.085	20.4	10.3	0.0129244
2	4.297	50	5.9	0.311059	5	2.8693	8.2	7.2	4.297	50	5.1	0.315505
3	4.55	87	10.3	0.0646775	3	0.390152	1.1	1	4.55	87	3.4	0.0646479
4	4.851	131	15.5	0.0327824	3	0.19603	0.6	0.5	4.851	130.6	3.3	0.0334414
5	5.077	164	19.4	0.0306701	6	0.322173	0.9	0.8	5.077	165.4	6.3	0.0286757
6	5.2	182	21.5	0.0668353	6	0.635744	1.8	1.6	5.2	182	5.8	0.0616047
7	5.336	204	24.1	0.210374	3	1.1963	3.4	3	5.336	204.4	3.2	0.210345
8	5.46	224	26.4	0.0194183	7	0.18206	0.5	0.5	5.46	226	6.6	0.0155601
9	5.59	245	28.9	0.054576	12	1.42833	4.1	3.6	5.59	250	12	0.0671025
10	5.695	262	30.9	0.316755	5	2.49856	7.1	6.3	5.695	262.1	4.8	0.292048
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.85	287	33.9	0.110959	5	0.899784	2.6	2.3	5.85	287	4.9	0.104464
12	5.994	314	37.1	0.528774	3	2.71559	7.7	6.8	5.994	314.3	2.9	0.533028
13	6.079	330	39	0.273653	3	1.46446	4.2	3.7	6.079	329.6	3	0.273177
14	6.169	347	41	0.24972	5	2.10856	6	5.3	6.169	346.5	4.8	0.247759
15	6.259	364	43	0.068145	6	0.74865	2.1	1.9	6.259	363.1	5.9	0.0710843
16	6.35	381	45	0.0924366	3	0.630813	1.8	1.6	6.35	381.9	3.5	0.101898
17	6.461	402	47.5	0.0977638	8	1.26347	3.6	3.2	6.461	401	7.6	0.0943946
18	6.499	409	48.3	0.151973	2	0.50495	1.4	1.3	6.499	409.5	2.2	0.126776
19	6.553	419	49.5	0.0707411	3	0.340275	1	0.9	6.553	419.3	2.7	0.0720145
20	6.666	430	50.8	0.485382	4	2.9576	8.4	7.5	6.666	430.5	3.5	0.473332
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.799	443	52.3	0.355474	2	1.48991	4.2	3.8	6.799	442.6	2.4	0.355289
22	6.916	457	54	0.117071	7	1.38942	4	3.5	6.916	454.7	6.9	0.112877
23	7.019	471	55.6	0.0191029	1	0.0334071	0.1	0.1	7.019	471.8	1.4	0.0137802
24	7.1	482	56.9	0.0141897	2	0.0607943	0.2	0.2	7.1	482.6	2.3	0.0147799
25	7.181	493	58.2	0.0125194	2	0.0395627	0.1	0.1	7.181	493	1.8	0.0126691
26	7.284	507	59.9	0.231037	3	1.18384	3.4	3	7.284	507.2	3	0.225667
27	7.35	516	60.9	0.0412538	4	0.326505	0.9	0.8	7.35	515.4	4.3	0.0432198
28	7.457	533	62.9	0.01333	3	0.0780029	0.2	0.2	7.457	532.7	3.3	0.0132836
29	7.696	571	67.4	0.0161667	4	0.0912348	0.3	0.2	7.696	570.8	3.7	0.0139743
30	7.854	596	70.4	0.482484	5	3.85765	11	9.7	7.854	596.5	5.1	0.430882
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.15	643	75.9	0.0603213	12	0.995227	2.8	2.5	8.15	640	12.3	0.0456691
32	8.45	711	83.9	0.0447468	5	0.400949	1.1	1	8.45	709.8	4.9	0.0459528
33	8.65	760	89.7	0.0392467	4	0.26198	0.7	0.7	8.65	759.4	3.8	0.0387778
34	9.3	816	96.3	0.118335	7	1.35995	3.9	3.4	9.3	815.2	6.7	0.114455

PeakCounter: 34

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.095	20	2.4	0.0304971	5	0.249746	0.7	0.7	4.095	19.2	4.7	0.0301976
2	4.297	51	6	0.324559	6	3.4045	9.6	8.9	4.297	51.3	5.8	0.329667
3	4.55	90	10.6	0.0311814	4	0.19526	0.5	0.5	4.55	90.1	3.5	0.031085
4	4.862	138	16.3	0.0427367	4	0.27353	0.8	0.7	4.862	137.4	3.6	0.0432559
5	5.031	164	19.3	0.0503565	8	0.694225	2	1.8	5.031	165.8	8.4	0.046745
6	5.2	190	22.4	0.0432847	10	0.720341	2	1.9	5.2	189.7	10.5	0.0387684
7	5.347	213	25.1	0.320291	3	1.54667	4.4	4	5.347	212.7	2.8	0.310003
8	5.455	230	27.1	0.0168063	5	0.119747	0.3	0.3	5.455	231	4.7	0.0144318
9	5.582	250	29.4	0.0528001	11	1.14599	3.2	3	5.582	254.8	11.4	0.0565673
10	5.697	268	31.6	0.367117	4	2.70064	7.6	7.1	5.697	268	4.4	0.348968
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.85	292	34.4	0.0572783	8	0.707836	2	1.9	5.85	294.1	7.8	0.0514701
12	6	319	37.6	0.571706	3	3.10838	8.7	8.1	6	319.3	3.2	0.548427
13	6.083	334	39.3	0.317746	4	2.01909	5.7	5.3	6.083	334.2	3.7	0.305323
14	6.177	351	41.3	0.243747	5	2.08087	5.9	5.4	6.177	350.2	4.7	0.247452
15	6.254	365	43	0.0353599	4	0.247619	0.7	0.6	6.254	366.4	3.8	0.0368372
16	6.36	384	45.2	0.117561	3	0.702775	2	1.8	6.36	384.1	3.3	0.12194
17	6.443	399	47	0.055478	9	0.994172	2.8	2.6	6.443	402.3	8.6	0.0649295
18	6.504	410	48.3	0.153488	2	0.560787	1.6	1.5	6.504	410.2	2.5	0.126943
19	6.587	422	49.7	0.0851383	6	0.874611	2.5	2.3	6.587	424.5	6.3	0.0787344
20	6.698	433	51	0.427696	4	2.83488	8	7.4	6.698	433.5	3.7	0.430468
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.799	443	52.2	0.341595	2	1.508	4.2	3.9	6.799	443.4	2.5	0.340721
22	6.905	456	53.7	0.125452	4	0.962041	2.7	2.5	6.905	456.3	4.2	0.130752
23	7.008	471	55.5	0.0152443	3	0.0794924	0.2	0.2	7.008	471.5	2.8	0.0160669
24	7.097	484	57	0.0166479	2	0.069295	0.2	0.2	7.097	484.6	2.2	0.0175044
25	7.186	497	58.5	0.0209063	2	0.0915671	0.3	0.2	7.186	496.6	2.4	0.0211118
26	7.282	511	60.2	0.261997	3	1.38056	3.9	3.6	7.282	511	3.1	0.254186
27	7.35	521	61.4	0.0241873	3	0.15059	0.4	0.4	7.35	520.4	3.4	0.0251862
28	7.45	537	63.3	0.0134453	4	0.105302	0.3	0.3	7.45	536.1	4.4	0.0134736
29	7.706	578	68.1	0.0170937	3	0.0885168	0.2	0.2	7.706	577.5	3	0.016687
30	7.769	588	69.3	0.0484459	2	0.194496	0.5	0.5	7.769	588.9	1.9	0.0573036
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.85	601	70.8	0.390984	7	4.02795	11.3	10.5	7.85	602	6.7	0.339544
32	8.15	649	76.4	0.0236659	3	0.119522	0.3	0.3	8.15	649.1	3.2	0.0207955
33	8.45	716	84.3	0.0152485	5	0.131422	0.4	0.3	8.45	717.1	4.6	0.0160806
34	8.65	764	90	0.0196377	5	0.157117	0.4	0.4	8.65	763.4	4.8	0.0186043
35	9.3	818	96.3	0.0674496	11	1.28425	3.6	3.4	9.3	814.1	11.1	0.0650802

PeakCounter: 35

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	81	9.5	0.0885677	4	0.606712	5.7	4.7	4.55	81.1	4	0.0858469
2	5.2	175	20.6	0.16252	4	1.14589	10.8	8.8	5.2	175.2	4.1	0.158199
3	5.85	284	33.4	0.184641	4	1.15616	10.9	8.9	5.85	283.6	3.5	0.185324
4	6.55	418	49.2	0.161555	6	1.57097	14.8	12.1	6.55	416.9	5.9	0.149787
5	6.85	448	52.7	0.0326472	4	0.231904	2.2	1.8	6.85	448.1	4.2	0.0314117
6	7.35	518	60.9	0.0802786	4	0.547756	5.2	4.2	7.35	517.8	3.9	0.0784854
7	8.15	645	75.9	0.112305	6	1.21704	11.5	9.3	8.15	644.9	6.3	0.109786
8	8.45	711	83.6	0.106094	6	1.18635	11.2	9.1	8.45	710.9	6.4	0.104885
9	8.65	762	89.6	0.088878	4	0.630452	5.9	4.8	8.65	761.8	4	0.0878822
10	9.3	823	96.8	0.221807	6	2.32687	21.9	17.9	9.3	821.8	5.9	0.223751

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO CO MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 158

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 7.100 5.200 10.300 5.850 11.200 6.550 13.500 6.850 3.100 7.350 5.000 8.150 11.900 8.450 11.400 8.650 5.800 9.300 20.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 1 LANE TYPE: pI Markers ACTUAL GEL LANE: 1 PeakCount: 10 Return to Lane Navigator
4.084 0.600 4.303 8.200 4.550 1.700 4.847 0.800 5.023 5.100 5.200 3.100 5.335 3.400 5.451 0.100 5.574 2.200 5.672 2.300 5.850 2.500 6.017 7.800 6.109 3.900 6.212 5.600 6.298 1.200 6.379 1.200 6.444 1.300 6.514 1.900 6.675 9.700 6.806 4.400 6.920 2.000 7.292 3.400 7.350 1.000 7.457 0.700 7.717 0.200 7.800 2.800 7.854 10.000 7.954 5.500 8.150 1.800 8.450 1.400 8.650 1.100 9.300 3.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 2 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 2 PeakCount: 32 Return to Lane Navigator
4.087 1.100 4.315 9.600 4.861 1.200 5.041 7.700 5.368 4.500 5.486 0.600 5.611 3.800 5.710 2.600 5.879 0.400 5.917 0.500 6.024 8.200 6.109 5.700 6.206 5.900 6.281 0.800 6.372 1.900 6.500 2.800 6.659 10.700 6.782 5.100 6.897 3.500 7.007 0.400 7.096 0.200 7.177 0.300 7.287 4.000 7.462 0 7.717 0.600 7.795 0.600 7.911 17.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 27 Return to Lane Navigator
4.550 7.600 5.200 9.600 5.850 11.800 6.550 13.600 6.850 1.800 7.350 6.100 8.150 11.900 8.450 10.400 8.650 5.200 9.300 21.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.085 0.700 4.297 8.200 4.550 1.100 4.851 0.600 5.077 0.900 5.200 1.800 5.336 3.400 5.460 0.500 5.590 4.100 5.695 7.100 5.850 2.600 5.994 7.700 6.079 4.200 6.169 6.000 6.259 2.100 6.350 1.800 6.461 3.600 6.499 1.400 6.553 1.000 6.666 8.400 6.799 4.200 6.916 4.000 7.019 0.100 7.100 0.200 7.181 0.100 7.284 3.400 7.350 0.900 7.457 0.200 7.696 0.300 7.854 11.000 8.150 2.800 8.450 1.100 8.650 0.700 9.300 3.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 34 Return to Lane Navigator
4.095 0.700 4.297 9.600 4.550 0.500 4.862 0.800 5.031 2.000 5.200 2.000 5.347 4.400 5.455 0.300 5.582 3.200 5.697 7.600 5.850 2.000 6.000 8.700 6.083 5.700 6.177 5.900 6.254 0.700 6.360 2.000 6.443 2.800 6.504 1.600 6.587 2.500 6.698 8.000 6.799 4.200 6.905 2.700 7.008 0.200 7.097 0.200 7.186 0.300 7.282 3.900 7.350 0.400 7.450 0.300 7.706 0.200 7.769 0.500 7.850 11.300 8.150 0.300 8.450 0.400 8.650 0.400 9.300 3.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 6 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 6 PeakCount: 35 Return to Lane Navigator
4.550 5.700 5.200 10.800 5.850 10.900 6.550 14.800 6.850 2.200 7.350 5.200 8.150 11.500 8.450 11.200 8.650 5.900 9.300 21.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Red Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Red Snapper ---- Go to Display Gel Lane Navigator.