US 7,393,630 B2
Use of microparticles combined with submicron oil-in-water emulsions
Derek O'Hagan, Berkeley, Calif. (US); Gary Van Nest, El Sobrante, Calif. (US); Gary S. Ott, Oakland, Calif. (US); and Manmohan Singh, Hercules, Calif. (US)
Assigned to Novartis Vaccines and Diagnostics, Inc., Emeryville, Calif. (US)
Filed on Feb. 08, 2005, as Appl. No. 11/53,290.
Application 09/564416 is a division of application No. 09/015736, filed on Jan. 29, 1998, granted, now 6,086,901.
Application 11/053290 is a continuation of application No. 10/211025, filed on Aug. 02, 2002, granted, now 6,855,492.
Application 10/211025 is a continuation of application No. 09/935466, filed on Aug. 20, 2001, granted, now 6,458,370.
Application 09/935466 is a continuation of application No. 09/564416, filed on May 02, 2000, granted, now 6,306,405.
Claims priority of provisional application 60/069724, filed on Dec. 16, 1997.
Prior Publication US 2005/0136073 A1, Jun. 23, 2005
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/70 (2006.01); A61K 39/21 (2006.01)
U.S. Cl. 435—5  [424/208.1] 17 Claims
 
1. A method of making a composition comprising combining a submicron oil-in-water emulsion with a selected antigen entrapped in, or adsorbed to, a biodegradable microparticle, wherein the microparticle is formed from a poly(α-hydroxy acid) selected from the group consisting of poly(L-lactide), poly(D,L-lactide) and poly(D,L-lactide-co-glycolide).