Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
European John Dory  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Zeus faber
FDA Seafood List Common Name: European John Dory
FDA Seafood List Market Name: Dory
Unique IEF Gel Code: 19950809gel05
Unique Web Code: zsh01Srr
Tissue Aliquot Code: zeusfabe I94-002 #01a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.556580.032931360.3731448.16.64.5566.46.50.0325639
25.215519.10.032176260.3307357.25.85.2155.86.10.0306734
35.8525931.90.028950660.3116286.85.55.85259.16.30.0279474
46.5538046.70.0326178100.5561112.19.86.55380.3100.031374
56.8541651.20.012925870.1561383.42.76.85416.370.0125345
67.3547758.70.023027470.2677495.84.77.35474.86.90.021832
78.1559773.40.057891570.71134715.512.58.15597.17.40.0545812
88.4565680.70.078004270.99150221.617.48.45655.57.10.0793296
98.6571587.90.066152950.53387511.69.48.65715.24.50.0663209
109.377595.30.019503120.3601577.86.39.3774120.0169645
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.14810.010287920.03862810.10.14.148.82.10.0103731
24.2562430.022789650.2043150.70.64.25623.15.20.0222048
34.474546.70.23707872.847231094.47453.970.23021
44.7429111.20.174709184.9936817.515.84.74287.1180.156524
55.51720425.20.013666330.06959220.20.25.5172032.70.014551
65.60621826.90.15649530.7576752.72.45.606217.82.70.158591
75.90526632.80.006374920.02356550.10.15.905265.31.80.007372
85.94527333.70.01044120.03736340.10.15.945272.72.10.0100086
96.01928635.30.036445720.1363810.50.46.019287.11.70.0460111
106.07729636.50.45777732.18677.76.96.077296.32.70.457054
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.16331138.30.28603941.763786.25.66.163311.33.60.279486
126.2232139.60.067730291.214974.33.96.22324.79.10.0750648
136.333541.30.42849532.444668.67.86.3334.63.30.416294
146.37534842.90.042630140.3210821.116.375350.14.40.0408841
156.518373460.014494320.05360180.20.26.51837320.0153956
166.69139548.70.52090132.9240310.29.36.691394.73.20.521781
176.82941150.70.045804760.4592151.61.56.829412.15.80.0449353
187.116446550.010668760.09690630.30.37.116444.360.00908
197.25646357.10.042019350.4031891.41.37.256461.65.40.0419117
207.37347858.90.082542450.663852.32.17.373477.14.80.0774236
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.4448860.20.16963361.7066665.47.44487.85.90.163935
227.61451463.40.036137530.1879910.70.67.61451430.0350292
237.74853465.80.24508541.576845.557.748533.53.60.245372
247.88255468.30.0702098181.863336.55.97.882562.2180.0584051
258.27261876.20.078206491.179684.13.78.272618.290.0739465
268.32362877.40.061088250.3853661.41.28.323629.850.0431142
PeakCounter: 26
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55668.10.030743550.2812486.44.5566.45.20.0304843
25.215519.10.02593460.2668517.66.15.21555.90.0256712
35.8525631.60.0182406160.48528113.911.15.85248.415.90.0172377
46.5537946.70.0266309120.48487413.811.16.55376.211.80.0231619
56.8541350.90.010902160.1357913.93.16.85414.66.40.011897
67.3547458.40.019628780.2344096.75.37.35472.87.90.016655
78.1559373.10.04429660.43057412.39.88.15592.65.70.0424679
88.4565280.40.055829260.62658817.914.38.45651.16.30.0561261
98.6571087.50.050976650.4111.79.48.65709.94.70.0496296
109.376894.70.0104815100.1477364.23.49.3768.59.60.008722
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.318253.10.009382760.09385570.70.64.31824.360.0087895
24.55617.50.12753771.5316710.89.64.5560.76.90.125091
34.7759611.80.0796153141.6937411.910.64.7759814.20.0672778
45.2162200.013846450.1174770.80.75.2160.45.10.0129836
55.54921326.20.053499930.28100721.85.549212.72.90.0539884
65.8525731.70.01218990.2051611.41.35.85260.290.0128076
75.88926432.50.012983220.003028005.8892662.40.0007072
85.957276340.005219820.01848740.10.15.9572762.10.0048725
96.0328935.60.052570730.27750621.76.03288.92.90.0531264
106.08629936.80.24699931.4468110.296.086298.93.30.244461
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.17631538.80.20304241.42056108.96.176315.340.198594
126.31634041.90.32633931.5896211.29.96.316339.72.70.330792
136.38335243.30.024102740.14235610.96.383352.23.50.0229013
146.51837646.30.019009860.1660071.216.518376.15.70.0163845
156.69239748.90.36882331.8737213.211.76.692396.92.90.365575
166.85414510.023818350.223791.61.46.85413.75.30.0239163
177.11244855.20.010504680.1182740.80.77.112445.680.0083394
187.227463570.015451740.1131330.80.77.227462.34.30.0148565
197.35479590.051926240.3744562.62.37.35477.14.50.0471209
207.42749060.30.069626791.010037.16.37.427490.690.063096
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.60751663.50.018767560.1817111.31.17.607516.460.0169962
227.747536660.11832240.7764135.54.97.747536.13.80.11517
238.1559473.20.020489950.1801921.31.18.15592.95.20.0196734
248.4565680.80.019580150.1526371.118.45655.54.50.0189674
258.6571287.70.024100140.1774531.21.18.65711.24.30.0233283
269.376994.70.005116370.05560760.40.39.3770.16.90.0045317
PeakCounter: 26
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.267232.80.019570970.2202330.80.74.26721.96.50.0190724
24.483556.80.20957982.8038410.59.14.48354.77.70.206092
34.7599611.80.122764183.5406213.311.54.75987.8180.110979
45.48220425.10.020390930.09536650.40.35.482204.52.60.0209
55.58822027.10.16685130.79470432.65.588219.62.70.16906
65.90526933.10.01056220.04114040.20.15.905268.920.0114621
75.95127734.10.009646120.03613250.10.15.9512772.10.0098361
86.02529035.70.088197320.3911131.51.36.025289.92.40.0900975
96.07729936.80.44630532.350448.87.66.077298.62.90.457665
106.15731338.50.26832631.550195.856.157313.53.30.268519
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.29533741.50.39508942.67359108.76.295337.13.70.411641
126.37635143.20.042478140.3409831.31.16.376352.54.50.0431687
136.51437546.20.020038530.09404520.40.36.514375.12.60.0202156
146.69639748.90.52424333.0607411.59.96.696396.93.30.524326
156.83641250.70.042396650.367761.41.26.836413.24.90.0426964
167.08844454.70.018783450.1753090.70.67.088444.55.20.0190007
177.24546457.10.040936550.3494611.31.17.245462.34.80.0408157
187.34647758.70.10995651.0686443.57.346478.15.30.113448
197.42848960.20.13941551.18524.43.87.428488.94.90.135908
207.61151663.50.081944791.169364.43.87.611516.29.50.0695162
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.75353766.10.27817541.850126.967.753536.43.80.275881
228.27562176.50.0929245152.511929.48.28.275624.815.30.0923465
PeakCounter: 22
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55718.70.041743960.4021578.56.34.5570.95.70.0400684
25.2163200.028001970.3399147.25.35.2161.46.90.0277721
35.8526432.40.023426670.2741375.84.35.85263.67.40.0207895
46.5538347.10.039589580.524371118.26.55382.680.036988
56.85415510.017084760.162053.42.56.85416.35.80.015811
67.3547858.70.024087270.2778965.84.37.35477.36.70.0235487
78.1559973.60.049477790.70498514.8118.155998.50.0465539
88.45659810.081732381.1820924.918.48.45657.98.30.0803974
98.6571487.70.082203140.62862413.29.88.65713.84.30.0819885
109.377194.70.0180527100.2546625.449.3770.49.80.0146644
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 104
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 26 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 26 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 22 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln