Mayer K, Cu-Uvin S, Hogan J, Rodriguez I, Tucker L, Allsworth J, Anderson D; International Conference on AIDS.
Int Conf AIDS. 1998; 12: 432 (abstract no. 23450).
Brown University AIDS Program, Providence, Rhode Island, USA.
OBJECTIVES: To describe clinical factors associated with genital tract HIV DNA, or RNA detection among ASX US women, with and without GTI. DESIGN: 89 ASX HIV + women enrolled in a prospective HIV natural history study (the HERS cohort) underwent a CV lavage which was analyzed for HIV DNA, cell-free (CF) and cell-associated (CA) HIV RNA, as well as genital tract WBC, and screened for syphilis, GC, chlamydia, trichomonas, and CV CMV culture.. METHODS: CV lavage utilized 10 ml PBS instilled during a speculum exam. CV WBC and STD studies were done on site; frozen CV and blood specimens were analyzed in batch using HIV RNA and DNA PCR probes. RESULTS: The women's blood CD4 counts ranged from 4 to 1,911 cells/mm3 (median: 310); CVL WBC counts ranged from 0 to 5,800,000 cells/mm3 (median: 215,000) with less than 1,000 RBC/CVL. Although HIV DNA was detected in 89% of blood samples (median copies/ml: 450; maximum: 6,000), HIV DNA or RNA was detected in only 51% of CV samples (maximum copies/ml: CV DNA: 20K; CF RNA: 240K; CA RNA 140K). Women who were HIV DNA (+) in blood more often had CV HIV DNA detected (p < 0.05). 2 women who had no blood HIV DNA detected, had CV CF RNA detected. Women with > 100,000 CV WBC/ml were (-) for other STD screening (including CMV culture) except for 1 (+) for trichomonas; but 57% had CV HIV DNA or RNA detected. Women with CV WBC counts > 100,000 cells/mm3 more often had CV CF RNA detected than those who did not (OR = 2.18, 95% C.I. 0.73, 7.06). Women with detectable CV HIV DNA or RNA had lower median CD4 counts (p = 0.03). CONCLUSIONS: Asymptomatic HIV + women with lower CD4 counts or GTI more often had CV HIV DNA or RNA detected. GTI in HIV + women was common and independent of other STDs. HIV may be detected in CV secretions as cell-free RNA, or cell-associated DNA or RNA, and women with undetectable HIV plasma viremia may have genital HIV detected.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Anti-HIV Agents
- CD4 Lymphocyte Count
- Female
- HIV
- HIV Antibodies
- HIV Core Protein p24
- HIV Envelope Protein gp120
- HIV Infections
- HIV Protease Inhibitors
- HIV Seronegativity
- HIV Seropositivity
- Humans
- Prospective Studies
- RNA
- RNA, Viral
- Viremia
- immunology
Other ID:
UI: 102229078
From Meeting Abstracts