![]() |
---|
Slicing Device: The cutting system can best be described as a modified microtome. Associated equipment included:
Blade, cutter assembly
Cutter assembly
Insulated box and cover
Ice remover
Cold plate assembly
Teflon tubing
Push rod
Digital distance travel indicator
Liquid nitrogen supply
A cold plate was placed inside an insulated box, and liquid nitrogen (LN2) was connected to the cooling system. The slicing fixture was then mounted on the cold plate. A 17.8 cm length of Teflon tubing, 0.635 cm inside diameter by 0.715 cm outside diameter, was inserted into the slicing fixture and installed so that it extended 1.27 cm out of the end of the fixture. The razor blade was then placed into its slot and used to trim the Teflon tube to length. An extractor fixture was placed on the protruding end of the Teflon which was inserted into the extractor fixture. Thermocouples located at the right, center and left of the cutter assembly, the sample stage, the LN2 inlet and outlet, the flow bench air, the cooling block, the sample transfer system and the box air were then secured in place. A plexiglass cover was placed on the insulated box, and the flow of liquid nitrogen began. The temperature of the fixture was reduced to between -50 and -70 degrees Celsius before operations continued.
Experiment + Electrophoresis Technology (MA-011)