Kellogg JA, Bankert DA; American Society for Microbiology. General Meeting.
Abstr Gen Meet Am Soc Microbiol. 1999 May 30-Jun 3; 99: 133 (abstract no. C-140).
York Hospital, PA.
Because of the increasing frequency with which a variety of Mycobacterium species are being recovered from immunosuppressed and other patients, as well as the technical complexity of many of the standard biochemical identification tests and the costs of nucleic acid probes, there is a growing need for a rapid, cost-effective alternative for identifying clinical isolates of mycobacteria. Therefore, the abitlity of the Sherlock Mycobacteria Identification System (MIDI Inc.) using computerized software (version 2.95) and a Hewlett Packard Series 1100 High Performance Liquid Chromatograph to identify mycobacteria to the species level was compared to identification using probes (Gen Probe) and biochemical tests. Clinical isolates and stock cultures were inoculated to Middlebrook 7H10 agar and incubated at 35 degrees C in 5- 10% C02. Harvesting, saponification, extraction, derivitization, and chromatography were completed following MIDI's instructions. Of 85 isolates or stock cultures, 60(71%)were given species names (52[87%]) of which were correct) and 25(29%) were unidentified by Sherlock. The overall predictive value for accuracy for Sherlock species identification was 87%, including 100% for both M. avium complex (19 of 19) and M. gordonae (14 of 14). The Sherlock Mycobacteria Identification System, combined with a knowledge of colony morphology, pigment, and growth rate, offers a rapid, reasonably accurate, cost-effective alternative to identification of many mycobacterial species using traditional biochemical tests and nucleic acid probes.
Publication Types:
Keywords:
- Chromatography, High Pressure Liquid
- DNA Probes
- Humans
- Mycobacterium
- Mycobacterium Infections
- Nucleic Acid Probes
- Software
- instrumentation
- psychology
Other ID:
UI: 102195575
From Meeting Abstracts