Technical Abstract: Three major species of root-knot nematode infect peanut (Arachis hypogaea): Meloidogyne arenaria race 1 (Ma), M. hapla (Mh), and M. javanica (Mj). Sources of resistance to all three are needed to develop novel peanut cultivars with broad resistance to Meloidogyne spp. Fifty-seven cultivars and breeding lines of peanut were collected from China and the US and evaluated in the greenhouse for resistance to Ma, Mh, and Mj. On lines 990304-1, 0029-2, 0031, 0108. and C724-25-8, all three root-knot species produced 25% of the eggs produced on susceptible controls, Georgia Green (for Ma and Mj) or D098 (for Mh). Based on egg numbers per gram of root, COAN, NemaTAM, and D009 showed high or moderate resistance to Ma and Mj; D099 had moderate resistance to both Ma and Mb; 0999 and 950530 had moderate resistance to Mj and Mh, while three and five accessions had moderate resistance to Ma and Mh alone, respectively C724-19-11 and ATO81 7 also showed some resistance to Ma, Mj, or Mh, however, the variability of egg numbers was high, indicating that these two accessions were still segregating. All Mj resistant genotypes were also resistant either to Ma, Mh, or both. For all three species, the correlation coefficients between gall number and eggs/g root were significantly positive (P All the genotypes which showed resistance based on egg production were also resistant on gall number, with two exceptions in Ma. Conversely, some genotypes were moderately or highly susceptible based on eggs/g root, although they were resistant based on gall number In conclusion, sources of resistance to all three Meloidogyne spp. exist within cultivated peanut (Arachis hypogaea L.) either with or without introgressed genes from peanut wild species.