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Title Quantification of deposition of neutrophilic granulocytes on vascular grafts in dogs with /sup 111/In-labeled granulocytes
Creator/Author Dewanjee, M.K. ; Solis, E. ; Mackey, S.T. ; Socher, S. ; Chowdhury, S. ; Wu, F.P. ; Kaye, M.P.
Publication Date1985 Mar 01
OSTI IdentifierOSTI ID: 5754544
Other Number(s)CODEN: MACPA
Resource TypeJournal Article
Resource RelationMayo Clin., Proc. ; Vol/Issue: 60:3
Research OrgMayo Clinic, Rochester, MN
Subject550601 -- Medicine-- Unsealed Radionuclides in Diagnostics; BLOOD VESSELS-- GRAFTS;BLOOD VESSELS-- SCINTISCANNING;NEUTROPHILS-- ADHESION; ARTERIES;BLOOD;DOGS;INDIUM 111;LABELLED COMPOUNDS;LEUKOCYTES;RETENTION;TRACER TECHNIQUES;VEINS
Related SubjectANIMALS;BETA DECAY RADIOISOTOPES;BIOLOGICAL MATERIALS;BLOOD;BLOOD CELLS;BLOOD VESSELS;BODY;BODY FLUIDS;CARDIOVASCULAR SYSTEM;COUNTING TECHNIQUES;DAYS LIVING RADIOISOTOPES;DIAGNOSTIC TECHNIQUES;ELECTRON CAPTURE RADIOISOTOPES;INDIUM ISOTOPES;INTERMEDIATE MASS NUCLEI;ISOMERIC TRANSITION ISOTOPES;ISOTOPE APPLICATIONS;ISOTOPES;LEUKOCYTES;MAMMALS;MATERIALS;MINUTES LIVING RADIOISOTOPES;NUCLEI;ODD-EVEN NUCLEI;ORGANS;RADIOISOTOPE SCANNING;RADIOISOTOPES;TRANSPLANTS;VERTEBRATES
Description/Abstract A new radioisotopic technique has been developed for quantification of deposition of neutrophilic granulocytes on vascular grafts.^Nine healthy mongrel dogs underwent bilateral femoral artery resection and reconstruction with grafts of the femoral vein and Gore-Tex.^Pure granulocytes that had been separated from whole blood by centrifugal elutriation were labeled with /sup 111/In-tropolone in plasma.^The granulocyte harvesting efficiency was 25 +/- 12%, and the labeling efficiency was 87 +/- 7%.^Three hours after injection of labeled granulocytes and 2 hours after reperfusion, the grafts were harvested and cut into several segments for study of areas of anastomoses and midsections.^On the basis of the radioactivity in the blood and in anastomotic and graft sections, the area of graft sections, and the neutrophilic granulocyte and differential leukocyte counts, the number of neutrophilic granulocytes adherent to a unit area and the total number of neutrophilic granulocytes on graft sections were calculated.^These quantifications of the deposition of neutrophilic granulocytes indicated that the midsections of Gore-Tex grafts retained more neutrophilic granulocytes than did the midsections of vein grafts.^Although the anastomotic areas retained more neutrophilic granulocytes than did the midsections of vein grafts, the opposite finding prevailed for the Gore-Tex grafts.^A major fraction of neutrophilic granulocytes on Gore-Tex grafts was incorporated into the thrombus.^Semiquantitative information obtained by scintigraphy of the deposition of neutrophilic granulocytes on vascular grafts also confirmed this observation.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 173-179
System Entry Date2001 May 13

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