Kim Y. Green, Ph.D.
Investigator
Description of Research Program
Norwalk virus and Norwalk-like viruses in the Caliciviridae are major etiologic agents of epidemic gastroenteritis. Numerous attempts to grow these viruses in cell culture or to develop animal models for the study of infection and disease have failed. The overall goals of this research program are to establish the role of these viruses as agents of human diarrheal disease and to develop experimental systems for study of the molecular basis for viral replication and pathogenicity.
Recent breakthroughs include the development of new diagnostic assays that use recombinant virus-like particles derived from expression of the major structural capsid protein from several different human calicivirus strains. Our recent epidemiologic studies have increased knowledge of the scope of these antigenically diverse viruses as agents of acute gastroenteritis in several settings, including nursing homes and U.S. military operations.
An additional breakthrough was the first report of an infectious cDNA clone for a calicivirus genome. These studies employed the feline calicivirus (FCV), a member of the Caliciviridae that replicates efficiently in cultured cells. FCV has become an important model system in our laboratory for the establishment of experimental approaches for study of the noncultivatable human caliciviruses.
Studies in progress include mapping genetic determinants in FCV and human caliciviruses involved in cell tropism and growth restriction, and study of the basic features of the viral replication cycle using infectious cDNA clones.
Memberships
- American Society for Virology
- American Society for Microbiology
Editorial Boards
Research Group Members
Stanislav Sosnovstev, Adriene King.
Selected Publications
(View current list in PubMed.)
Green KY, Belliot G, Taylor JL, Valdesuso J, Lew JF, Kapikian AZ, Lin FC. A predominant role for Norwalk-like viruses as agents of epidemic gastroenteritis in Maryland nursing homes for the elderly. Journal of Infectious Diseases. 2002. 185(2): 133-146.
Lew JF, Kapikian AZ, Jiang X, Estes MK, Green KY. Identification of minireovirus as a Norwalk-like virus in pediatric patients with gastroenteritis. Journal of Virology. 1994. 68: 3391-3396.
Lew JF, Kapikian AZ, Valdesuso J, Green KY. Molecular characterization of Hawaii virus and other Norwalk-like viruses: Evidence for genetic polymorphism among human caliciviruses. Journal of Infectious Diseases. 1995. 170(3): 535-542.
Sosnovtsev S, Green KY. RNA transcripts derived from a cloned full-length copy of the feline calicivirus genome do not require VpG for infectivity. Virology. 1995. 210: 383-390.
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