RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Channel Catfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Ictalurus punctatus
FDA Seafood List Common Name: Channel Catfish
FDA Seafood List Market Name: Catfish
Unique IEF Gel Code: 19970206gel08
Unique Web Code: cch01Srr
Tissue Aliquot Code: ictapunc K95-004 #04p Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	110	13.1	0.0651593	10	0.940873	8.5	5.7	4.55	106.7	10.2	0.0519033
2	5.2	202	24	0.132303	4	0.847499	7.6	5.1	5.2	202.3	3.8	0.125367
3	5.85	326	38.8	0.230443	5	1.80722	16.3	11	5.85	326.4	4.8	0.213108
4	6.55	466	55.4	0.142166	6	1.46876	13.2	8.9	6.55	464.9	6.1	0.135453
5	6.85	496	59	0.0633713	5	0.501211	4.5	3	6.85	496.7	4.6	0.0609731
6	7.35	561	66.7	0.117799	4	0.822383	7.4	5	7.35	561	4	0.116049
7	8.15	685	81.5	0.0917415	10	1.69254	15.2	10.3	8.15	683.3	10.3	0.0930929
8	8.45	746	88.7	0.162156	7	2.08157	18.7	12.6	8.45	745.2	7.5	0.157094
9	8.65	794	94.4	0.12878	4	0.941952	8.5	5.7	8.65	793.2	4.2	0.125939

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.986	17	2	0.11953	6	1.11216	2.9	1.9	3.986	16.4	5.6	0.112398
2	4.48	94	11.2	0.595697	4	4.63161	12	7.7	4.48	94.2	4.4	0.600117
3	4.704	129	15.3	0.431455	4	3.16151	8.2	5.3	4.704	128.8	4.1	0.439548
4	4.864	154	18.3	0.0559759	12	0.966254	2.5	1.6	4.864	155.9	12.1	0.045147
5	4.979	172	20.4	0.454254	3	1.98408	5.1	3.3	4.979	171.8	2.6	0.435501
6	5.05	183	21.7	0.0143385	2	0.0299434	0.1	0	5.05	183.3	1.7	0.0101994
7	5.165	201	23.8	0.112382	3	0.60239	1.6	1	5.165	201	3.2	0.104706
8	5.225	211	25	0.0516465	8	0.678038	1.7	1.1	5.225	211.9	7.9	0.048266
9	5.722	302	35.8	0.111723	5	0.941373	2.4	1.6	5.722	301.1	4.8	0.110471
10	5.776	312	37	0.121784	4	0.805028	2.1	1.3	5.776	311.4	3.7	0.123817
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.842	324	38.4	0.502571	4	3.14978	8.1	5.3	5.842	323.8	3.6	0.497614
12	5.913	338	40.1	0.451451	4	3.29799	8.5	5.5	5.913	337.7	4.4	0.419835
13	5.984	352	41.8	0.128215	5	1.24424	3.2	2.1	5.984	351.7	5.4	0.129616
14	6.04	363	43.1	0.0280759	2	0.0619931	0.2	0.1	6.04	364.3	1.5	0.0231851
15	6.176	390	46.3	0.0279032	1	0	0	0	6.176	386	1	0
16	6.206	396	47	0.0708932	2	0.301342	0.8	0.5	6.206	396.1	2.4	0.0719363
17	6.272	409	48.5	0.125952	3	0.598761	1.5	1	6.272	408.8	2.7	0.124667
18	6.312	417	49.5	0.24639	3	1.05444	2.7	1.8	6.312	417.4	2.7	0.223397
19	6.363	427	50.7	0.0749497	9	0.91185	2.4	1.5	6.363	425.5	9.5	0.0542633
20	6.403	435	51.6	0.403908	2	1.21311	3.1	2	6.403	434.9	1.8	0.384706
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.454	445	52.8	0.513413	3	2.34635	6.1	3.9	6.454	444.9	2.5	0.520937
22	6.499	454	53.9	0.149223	3	0.663636	1.7	1.1	6.499	454.1	2.5	0.146991
23	6.753	485	57.5	0.0398349	7	0.366968	0.9	0.6	6.753	485.4	6.8	0.0304738
24	6.831	493	58.5	0.0624304	2	0.197918	0.5	0.3	6.831	492.8	2.2	0.0508405
25	6.905	502	59.5	0.400836	2	1.67639	4.3	2.8	6.905	502.5	2.3	0.41266
26	7.006	515	61.1	0.0454232	5	0.390603	1	0.7	7.006	516	5.1	0.0434355
27	7.303	553	65.6	0.0228924	2	0.0929692	0.2	0.2	7.303	552.6	2.3	0.022589
28	7.396	566	67.1	0.0479524	3	0.222625	0.6	0.4	7.396	566.3	2.6	0.0489116
29	8.671	797	94.5	0.202671	18	6.04268	15.6	10.1	8.671	795.3	18	0.189404

PeakCounter: 29

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	101	11.9	0.0591364	6	0.584307	4.9	3.7	4.55	100.7	5.8	0.0568757
2	5.2	212	25.1	0.0757843	9	1.02411	8.6	6.5	5.2	209.5	8.7	0.0667832
3	5.85	326	38.5	0.124711	5	1.07467	9	6.9	5.85	325.3	5	0.122015
4	6.55	463	54.7	0.168579	7	1.85333	15.5	11.8	6.55	462.2	6.8	0.153746
5	6.85	495	58.5	0.0679307	4	0.481648	4	3.1	6.85	495.4	4.4	0.0623144
6	7.35	558	66	0.121918	4	0.81493	6.8	5.2	7.35	558.3	3.8	0.122072
7	8.15	679	80.3	0.115169	12	2.42311	20.3	15.5	8.15	676.9	12.1	0.112844
8	8.45	743	87.8	0.181601	8	2.45447	20.5	15.7	8.45	742.3	7.9	0.174564
9	8.65	791	93.5	0.172715	4	1.24652	10.4	8	8.65	790.7	4.3	0.164278

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.035	18	2.1	0.0568182	4	0.417979	1.5	1.2	4.035	17.8	4.1	0.0569768
2	4.46	87	10.3	0.410181	5	3.34954	11.8	9.6	4.46	87.2	4.6	0.407507
3	4.657	119	14	0.203012	5	1.81157	6.4	5.2	4.657	119.2	5	0.20584
4	4.823	146	17.2	0.0275064	8	0.417482	1.5	1.2	4.823	146.2	8.3	0.0285479
5	4.933	164	19.3	0.143168	6	1.44633	5.1	4.1	4.933	165.7	5.6	0.146327
6	5.143	198	23.3	0.0534505	7	0.645198	2.3	1.9	5.143	198	6.6	0.0552019
7	5.215	210	24.8	0.0156616	6	0.162284	0.6	0.5	5.215	212.7	6.2	0.0146565
8	5.693	295	34.8	0.0440622	3	0.27117	1	0.8	5.693	294	3.5	0.0441927
9	5.754	306	36.1	0.048547	2	0.183918	0.6	0.5	5.754	305.4	2.2	0.0461427
10	5.811	316	37.3	0.415083	4	2.66102	9.4	7.6	5.811	316.3	3.7	0.402139
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.89	331	39	0.371492	5	3.40217	12	9.8	5.89	330.6	5.4	0.354128
12	5.95	343	40.4	0.0852294	5	0.761808	2.7	2.2	5.95	344	4.9	0.0881961
13	6.025	358	42.2	0.0211257	2	0.0682685	0.2	0.2	6.025	358	1.9	0.0201339
14	6.185	390	46	0.051392	5	0.394637	1.4	1.1	6.185	389.2	5.4	0.0410408
15	6.25	403	47.5	0.0773263	3	0.346755	1.2	1	6.25	402.8	2.5	0.0770999
16	6.29	411	48.5	0.143993	3	0.684976	2.4	2	6.29	411.6	2.8	0.13993
17	6.34	421	49.6	0.0351115	6	0.281305	1	0.8	6.34	420	6.3	0.0252423
18	6.38	429	50.6	0.292064	2	0.944746	3.3	2.7	6.38	429	1.9	0.28658
19	6.43	439	51.8	0.470577	2	1.91718	6.8	5.5	6.43	439	2.3	0.470473
20	6.485	450	53.1	0.109945	4	0.640045	2.3	1.8	6.485	450.1	3.6	0.10153
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.825	492	58	0.0479687	5	0.419356	1.5	1.2	6.825	491.9	5.2	0.0454134
22	6.927	504	59.4	0.25335	2	1.04811	3.7	3	6.927	504.4	2.3	0.25543
23	7.035	517	61	0.0206341	3	0.118894	0.4	0.3	7.035	516.5	3.3	0.0200894
24	7.35	555	65.4	0.0703754	7	0.746243	2.6	2.1	7.35	556.1	6.7	0.062875
25	8.15	678	80	0.0758225	12	1.49525	5.3	4.3	8.15	675.4	11.5	0.0731712
26	8.45	743	87.6	0.0598464	5	0.556594	2	1.6	8.45	742.3	5.2	0.0603132
27	8.65	789	93	0.124336	16	3.16333	11.2	9.1	8.65	791.2	15.8	0.113031

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.01	19	2.2	0.115793	7	1.27544	3.1	1.8	4.01	18.8	6.8	0.105763
2	4.509	96	11.3	0.598321	4	4.65781	11.1	6.6	4.509	96	4.4	0.593246
3	4.749	133	15.6	0.419348	4	2.94965	7.1	4.2	4.749	132.5	3.9	0.421804
4	4.911	158	18.6	0.0559642	11	1.02072	2.4	1.4	4.911	159.9	11.2	0.0511997
5	5.021	175	20.6	0.337719	4	2.07085	5	2.9	5.021	174.9	3.5	0.33152
6	5.207	204	24	0.09804	6	0.881015	2.1	1.3	5.207	202.3	6	0.082925
7	5.257	213	25.1	0.0565026	7	0.680818	1.6	1	5.257	212.9	7.4	0.0517337
8	5.728	298	35.1	0.136743	4	1.01476	2.4	1.4	5.728	297.7	4.3	0.131728
9	5.789	309	36.4	0.1324	3	0.616323	1.5	0.9	5.789	308.2	2.8	0.125408
10	5.844	319	37.5	0.53995	4	3.27447	7.8	4.7	5.844	319.6	3.6	0.514727
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.919	334	39.3	0.420284	5	3.77428	9	5.4	5.919	333.5	5.3	0.399179
12	5.982	347	40.8	0.0924686	6	1.01277	2.4	1.4	5.982	348	6	0.0958131
13	6.051	361	42.5	0.038874	2	0.0816953	0.2	0.1	6.051	360.9	1.5	0.0305455
14	6.178	387	45.5	0.0372137	11	0.0747023	0.2	0.1	6.178	384.5	11.1	0.00380797
15	6.207	393	46.2	0.0785318	2	0.312317	0.7	0.4	6.207	392.7	2.2	0.07974
16	6.261	404	47.5	0.146413	2	0.653601	1.6	0.9	6.261	404.2	2.5	0.148146
17	6.305	413	48.6	0.260435	3	1.05401	2.5	1.5	6.305	413.4	2.6	0.225051
18	6.359	424	49.9	0.0520145	7	0.632085	1.5	0.9	6.359	420.4	7.1	0.0505418
19	6.393	431	50.7	0.439264	2	1.54063	3.7	2.2	6.393	430.5	1.9	0.447796
20	6.447	442	52	0.561985	3	2.5773	6.2	3.7	6.447	441.5	2.5	0.575987
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.496	452	53.2	0.188198	3	0.851195	2	1.2	6.496	451.6	2.6	0.188071
22	6.54	461	54.2	0.0357365	2	0.13498	0.3	0.2	6.54	461.3	2.1	0.037083
23	6.789	488	57.4	0.0487587	6	0.409188	1	0.6	6.789	487.3	6.5	0.0356657
24	6.873	497	58.5	0.06413	2	0.242533	0.6	0.3	6.873	496.1	2.2	0.0610579
25	6.949	506	59.5	0.396103	2	1.6741	4	2.4	6.949	505.8	2.3	0.403421
26	7.035	516	60.7	0.0420914	3	0.218657	0.5	0.3	7.035	516.3	2.8	0.043545
27	7.389	559	65.8	0.0658149	3	0.305493	0.7	0.4	7.389	559.3	2.7	0.062823
28	8.669	794	93.4	0.258061	18	7.79097	18.6	11.1	8.669	795	18	0.244203

PeakCounter: 28

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	104	12.2	0.0554443	5	0.466234	4.8	3.6	4.55	104.7	5	0.0528858
2	5.2	199	23.3	0.0750025	6	0.773169	8	6	5.2	198.6	5.9	0.0743013
3	5.85	318	37.3	0.135736	4	0.896944	9.3	7	5.85	317.7	3.8	0.131696
4	6.55	464	54.4	0.15203	6	1.52004	15.7	11.8	6.55	463.1	6.1	0.140654
5	6.85	495	58	0.063776	5	0.492611	5.1	3.8	6.85	495.9	4.6	0.0602054
6	7.35	552	64.7	0.119705	4	0.83316	8.6	6.5	7.35	551.6	4.1	0.115537
7	8.15	677	79.4	0.121247	9	1.81577	18.7	14.1	8.15	676.8	8.6	0.118691
8	8.45	742	87	0.172656	6	1.92875	19.9	15	8.45	741	6.5	0.167881
9	8.65	791	92.7	0.136779	4	0.967248	10	7.5	8.65	790.8	4.1	0.131538

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 111

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 8.500 5.200 7.600 5.850 16.300 6.550 13.200 6.850 4.500 7.350 7.400 8.150 15.200 8.450 18.700 8.650 8.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
3.986 2.900 4.480 12.000 4.704 8.200 4.864 2.500 4.979 5.100 5.050 0.100 5.165 1.600 5.225 1.700 5.722 2.400 5.776 2.100 5.842 8.100 5.913 8.500 5.984 3.200 6.040 0.200 6.176 0 6.206 0.800 6.272 1.500 6.312 2.700 6.363 2.400 6.403 3.100 6.454 6.100 6.499 1.700 6.753 0.900 6.831 0.500 6.905 4.300 7.006 1.000 7.303 0.200 7.396 0.600 8.671 15.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 29 Return to Lane Navigator
4.550 4.900 5.200 8.600 5.850 9.000 6.550 15.500 6.850 4.000 7.350 6.800 8.150 20.300 8.450 20.500 8.650 10.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.035 1.500 4.460 11.800 4.657 6.400 4.823 1.500 4.933 5.100 5.143 2.300 5.215 0.600 5.693 1.000 5.754 0.600 5.811 9.400 5.890 12.000 5.950 2.700 6.025 0.200 6.185 1.400 6.250 1.200 6.290 2.400 6.340 1.000 6.380 3.300 6.430 6.800 6.485 2.300 6.825 1.500 6.927 3.700 7.035 0.400 7.350 2.600 8.150 5.300 8.450 2.000 8.650 11.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 27 Return to Lane Navigator
4.010 3.100 4.509 11.100 4.749 7.100 4.911 2.400 5.021 5.000 5.207 2.100 5.257 1.600 5.728 2.400 5.789 1.500 5.844 7.800 5.919 9.000 5.982 2.400 6.051 0.200 6.178 0.200 6.207 0.700 6.261 1.600 6.305 2.500 6.359 1.500 6.393 3.700 6.447 6.200 6.496 2.000 6.540 0.300 6.789 1.000 6.873 0.600 6.949 4.000 7.035 0.500 7.389 0.700 8.669 18.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 28 Return to Lane Navigator
4.550 4.800 5.200 8.000 5.850 9.300 6.550 15.700 6.850 5.100 7.350 8.600 8.150 18.700 8.450 19.900 8.650 10.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Channel Catfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Channel Catfish ---- Go to Display Gel Lane Navigator.