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Title Simian virus 40 T antigen alters the binding characteristics of specific simian DNA-binding factors
Creator/Author Gallo, G.J. ; Gilinger, G. ; Alwine, J.C.
Publication Date1988 Apr 01
OSTI IdentifierOSTI ID: 7192066
Other Number(s)CODEN: MCEBD
Resource TypeJournal Article
Resource RelationMol. Cell. Biol. ; Vol/Issue: 8:4
Research OrgDept. of Microbiology and The Graduate Group of Molecular Biology, Univ. of Pennsylvania, School of Medicine, Philadelphia, PA (US)
Subject550200 -- Biochemistry ;550700 -- Microbiology; HELA CELLS-- GENE AMPLIFICATION;SIMIAN VIRUS-- ANTIGEN-ANTIBODY REACTIONS;SIMIAN VIRUS-- ANTIGENS;SIMIAN VIRUS-- ONCOGENES; BIOLOGICAL PATHWAYS;CHEMICAL BONDS;DNA;GENE RECOMBINATION PROTEINS;HOST-CELL REACTIVATION;ONCOGENIC TRANSFORMATIONS
Related SubjectBIOLOGICAL RECOVERY;BIOLOGICAL REPAIR;CELL TRANSFORMATIONS;GENES;MICROORGANISMS;NUCLEIC ACIDS;NUCLEOPROTEINS;ORGANIC COMPOUNDS;PARASITES;PROTEINS;RECOVERY;REPAIR;VIRUSES
Description/Abstract Although large T antigen is a well-characterized DNA-binding protein, a variety of data suggest that its trans-activation function does not require direct interaction with DNA.^ The authors demonstrate that defined late promoter elements, omega (..omega..), tau (tau), and delta (delta), necessary for T-antigen-mediated trans-activation, are binding sites for simian cellular factor, not T antigen.^ Two of the late promoter elements (..omega.. and tau) are shown to bind the same factor or family of factors.^ These factors bind to a site very similar to that for the HeLa cell factor AP1.^ The authors refer to these factors as the simian AP1-sequence recognition proteins (sAP1-SRPs).^ Compared with normal simian CV-1P cells, the sAP1-SRPs from T-antigen-producing COS cells, or from 14-h simian virus 40-infected CV-1P cells, showed altered binding patterns to both the ..omega.. and tau binding sites.^ In addition, the sAP1-SRPs from T-antigen-containing cells bound to the tau site more stably than did the analogous factors from normal CV-1P cells.^ The altered pattern of binding and the increased stability of binding correlated with the presence of T antigen in the cell.^ Additionally, the alteration of the binding pattern with 14 h of infection in CV-1P cells is temporally correct for late promoter activation.^ Overall, the data show (i) that the late promoter elements necessary for T-antigen-mediated trans-activation contain binding sites for simian cellular DNA-binding proteins; (ii) that the presence of T antigen causes alterations in the binding characteristics of specific simian cellular DNA-binding factors or families of factors; and (iii) that factors which bind to the late promoter elements required for activation have altered and more stable binding characteristics in the presence of T antigen.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 1648-1656
System Entry Date2001 May 13

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