MCF7
10X
20X
| ATCC | HTB-22 |
|---|---|
| Specimen Origin | |
| Source | Georgetown |
| Organism | Homo Sapiens (Human) |
| Tissue | Mammary gland |
| Tumourigenic | |
| Morphology | Epithelial |
| Age Status | 69 (years) |
| Ethnicity | Caucasian |
| Gender | Female |
| Cytogenetic Analysis | The stemline chromosome numbers ranged from hypertriploidy to hypotetraploidy, with the 2S component occurring at 1%. There were 29 to 34 marker chromosomes per S metaphase; 24 to 28 markers occurred in at least 30% of cells, and generally one large submetacentric (M1) and 3 large subtelocentric (M2, M3, and M4) markers were recognizable in over 80% of metaphases. No DM were detected. Chromosome 20 was nullisomic and X was disomic. |
| Growth Properties | Adherent |
| Doubling Time | |
| Culture Media | DMEM, 10%FBS |
| Split Ratio | A subcultivation ratio of 1:3 to 1:6 is recommended |
| Freeze Media | culture medium 95%; DMSO, 5% |
| Comments | The MCF7 line retains several characteristics of differentiated mammary epithelium including ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes. Contains the Tx-4 oncogene. Growth of MCF7 cells is inhibited by tumor necrosis factor alpha (TNF alpha). Secretion of IGFBP's can be modulated by treatment with anti-estrogens. |
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| Mutations |