Energy Citations Database

Bibliographic Citation

 
Document
For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field.
For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability.
Title Aneuploidy detection for chromosomes 1, X and Y by fluorescence in situ hybridization in human sperm from oligoasthenoteratozoospermic patients
Creator/Author Pang, M.G. ; Zackowski, J.L. ; Acosta, A.A. [Eastern Virginia Medical School, Norfolk, VA (United States)][and others]
Publication Date1994 Sep 01
OSTI IdentifierOSTI ID: 133733
Report Number(s)CONF-941009--
Other Number(s)AJHGAG; ISSN 0002-9297
Resource TypeJournal Article
Resource RelationAmerican Journal of Human Genetics ; VOL. 55 ; ISSUE: Suppl.3 ; 44. annual meeting of the American Society of Human Genetics, Montreal (Canada), 18-22 Oct 1994 ; PBD: Sep 1994
Subject55 BIOLOGY AND MEDICINE, BASIC STUDIES ; HETEROCHROMOSOMES; CHROMOSOMAL ABERRATIONS; ANEUPLOIDY; MUTATION FREQUENCY; DETECTION; SPERMATOZOA; MALES; FERTILITY; HUMAN CHROMOSOME 1; FLUORESCENCE; DNA HYBRIDIZATION; FERTILIZATION; PROBES
Description/Abstract Oligoasthenoteratozoospermic males (n=15) were investigated for infertility as compared with proven fertile donors. The oligoasthenoteratozoospermic population showed a mean sperm concentration of 9.7 x 10{sup 6}/ml (Range 4.2-19.7), mean motility of 38.5% (Range 10.6-76.8) and morphology (measured by the percentage of normal forms evaluated by strict criteria) with a mean of 3.49% (Range 1.5-5.0). Fluorescence in situ hybridization (FISH) using satellite DNA probes specific for chromosomes 1 (puc 1.77), X (alpha satellite), and Y (satellite-III at Yqh) was performed on human interphase sperm nuclei. DNA probes were either directly labelled with rhodamine-dUTP, FITC-dUTP, or biotinylated by nick translation. Hybridization and signal detection were done by routine laboratory protocols. Microscopic analysis was performed using a cooled CCD camera attached to an epi-fluorescent microscope. After hybridization, fertile donors yielded a frequency of 0.96% (n=12) nullisomic, 98.5% (n=1231) monosomic and 0.96% (n=12) disomic for chromosome 1, whereas oligoasthenoteratozoospermic males yielded a frequency of 16% (n=600) nullisomic, 74.5% (n=2792) monosomic and 9.9% (n=370) disomic. In addition, fertile donors yielded a frequency of 45.7% (n=633) monosomic and 0.7% (n=11) disomic for chromosome X, whereas oligoasthenoteratozoospermic males yielded a frequency of 38.7% (n=760) monosomic and 0.8% (n=13) disomic. Chromosome Y frequencies for fertile donors showed 44.6% (n=614) monosomic and 0.6% (n=2) disomic, whereas oligoasthenoteratozoospermic males yielded a frequency of 33.2% (n=701) monosomic and 0.8% (n=15) disomic. This suggests that the frequency of nullisomy for chromosome 1 is significantly higher (p<0.001) in sperm from oligoasthenoteratozoospermic makes versus sperm from our fertile donors. We conclude that FISH is a powerful tool to determine the frequency of aneuploidy in sperm from oligoasthenoteratozoospermic patients.
Country of PublicationUnited States
LanguageEnglish
Formatpp. A113.644 ; PL:
System Entry Date2001 May 03

Top