Newsletter 132 October 9, 2006 |
The
NIH X-Ray Diffraction
Interest Group
Newsletter
web site: http://mcl1.ncifcrf.gov/nihxray
The 2006 International Conference on Structural Genomics Oct. 22-26, 2006, Beijing, China Advances in Protein Crystallography 24 - 25 January 2007, South San Francisco, CA, USA 9th International Conference on Biology and Synchrotron Radiation 13-17 August 2007, Manchester, England Item 1: September 2006 Publications by
Members: 1: Gustchina E, Louis JM, Bewley CA, Clore GM. Item 2: Tips and Tricks - Crystallization Editorial - The Silver Bullets:
At the ACA 2006, Bob Cudney (Hampton Research) and Alexander McPherson
(University of California Irvine) presented an alternative stretage for
crystallizing macromolecules, as they put it, by searching the silver
bullets. Examples of the silver bullets incude hexammine cobalt (III)
chloride, 1,3-propanediol, sebacic acid, 4-aminobezonic acid,
terephthalic acid, arginine, pentaglycine, glycerol 2-phosphate,
trans-aconitic acid, trimesic acid, and putrescine. As you may realize,
they are in fact additives. They tested 120 additives in the
crystallization experiment of 81 proteins using two fundamental
conditions: (1) 30% w/v PEG 3350, 0.1 M HEPES pH 7.0; and (2) 50%
TacsimateTM pH 7.0. The succesful rate was very impressive:
65 out of 81 (85%) proteins crystallized. Most significant was that 35
of the 65 (54%) crystallized only in the presence of one or more
reagent mixes, but not in control samples lacking any additives!
Click for Introduction and tips and tricks in Crystallization, Post-crystallization treatments for improving diffraction quality of protein crystals, Derivatization, Diffraction, Symmetry, Structure Solution, Structure Refinement, and Structure Analysis.
Item 3: Topic Discussion - Low Resolution Crystallography Byron DeLaBarre & Axel Brunger: Considerations for the refinement of low-resolution crystal structures Click for previous discussions on: PHASER, HKL2000, Parallel Protein Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography, and Absorption Correction.
Item 4: Dr. Zbigniew Dauter's Lectures at the NIH (03/29-31/2005) |
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