Projects

Development of Methods for Rapid Control of Classical Swine Fever (Csf) Appropriated (D)   Accession Number: 411629

Foot-and-Mouth Disease Virus (Fmdv) Host-Pathogen Interactions Appropriated (D)   Accession Number: 411986

Role of Glycosylation of Csfv Envelope Proteins on Virus Virulence, Antigenicity and Immunogenicity Reimbursable (R)   Accession Number: 411177

Foreign Animal Disease Vaccines and Diagnostics Reimbursable (R)   Accession Number: 414498

Classical Swine Fever Virus: Role of the 3'Utr in Infectivity of Swine Specific Cooperative Agreement (S)   Accession Number: 409306

Assessment of the Role of Foot-and-Mouth Disease Virus (Fmdv) Proteins in Viral Pathogenesis Specific Cooperative Agreement (S)   Accession Number: 411126

Publications

Development of a Live Attenuated Antigenic Marker Classical Swine Fever Vaccine - (Trade Journal) - (15-Oct-08)

Role of the Region Located Between the Two AUG Initiation Codons in the Pathogenesis of Foot-and-Mouth Disease Virus (FMDV) - (Abstract)

Piccone, M.E., Pacheco Tobin, J., Pauszek, S.J., Borca, M.V., Rieder, A.E., Rodriguez, L.L. 2008. Role of the Region Located Between the Two AUG Initiation Codons in the Pathogenesis of Foot-and-Mouth Disease Virus (FMDV). European Study Group on the Molecular Biology of Picornaviruses. p. 199.

Removal of a N-linked Glycosylation Site on the Classical Swine Fever Virus Strain Brescia E(rns) Glycoprotein Affects Virulence in Swine - (Peer Reviewed Journal)

Fernandez-Sainz, I., Holinka, L.G., Lu, Z., Risatti, G.R., Borca, M.V. 2007. Removal of a N-linked Glycosylation Site on the Classical Swine Fever Virus Strain Brescia E(rns) Glycoprotein Affects Virulence in Swine. Virology. 370:122-129.

Sheeppox virus kelch-like gene SPPV-019 affects virus virulence - (Peer Reviewed Journal)

Balinsky, C.A., Delhon, G., Afonso, C.L., Risatti, G.R., Borca, M.V., French, R.A., Tulman, E.R., Geary, S.J., Rock, D.L. 2007. Sheeppox virus kelch-like gene SPPV-019 affects virus virulence. Journal of Virology. 81(20):11392-11401.

Genetic and Phenotypic Variation of FMDV During Serial Passages in a Natural Host - (Peer Reviewed Journal) - (24-Jul-07)

Classical Swine Fever Virus Inhibits Nitric Oxide Production in Infected Macrophages - (Peer Reviewed Journal)

Zaffuto, K.M., Piccone, M.E., Burrage, T.G., Risatti, G.R., Borca, M.V., Holinka, L.G., Rock, D.L., Afonso, C.L. 2007. Classical Swine Fever Virus Inhibits Nitric Oxide Production in Infected Macrophages. Journal of General Virology. 88:3007-3012.

Mutations in the Carboxyl Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus are Responsible for Viral Attenuation in Swine - (Abstract)

Risatti, G.R., Holinka, L.G., Fernanez-Sainz, I., Carrillo, C., Kutish, G., Lu, Z., Zhu, J., Rock, D.L., Borca, M.V., 2007, Mutations in the Carboxyl Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus are Responsible for Viral Attenuation in Swine. Positive Strand RNA Virus International Conference Proceedings. P2-K27, p.116

N-linked Glycosylation of Classical Swine Fever Virus Strain Brescia Erns Glycoprotein Alters Virulence in Swine - (Abstract)

Fernandez-Sainz, I., Holinka, L.G., Lu, Z., Risatti, G.R., Borca, M.V. 2007. N-linked Glycosylation of Classical Swine Fever Virus Strain Brescia Erns Glycoprotein Alters Virulence in Swine. Eighth International Symposium on Positive Strand RNA Virus, P2-K26, P.116

Mutations in the Carboxyl Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus is Responsible for Viral Attenuation in Swine - (Peer Reviewed Journal)

Risatti, G., Holinka, L.G., Fernandez-Sainz, I., Carrillo, C., Kutish, G.F., Lu, Z., Zhu, J., Rock, D.L., Borca, M.V. 2007. Mutations in the Carboxyl Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus is Responsible for Viral Attenuation in Swine. Virology. 364 (2): 371-82.

N-Linked Glycosylation Status Of Classical Swine Fever Virus Strain Brescia E2 Glycoprotein Influences Virulence In Swine - (Abstract)

Risatti, G., Holinka, L.G., Fernandez-Sainz, I., Carrillo, C., Lu, Z., Borca, M.V. 2006. N-linked glycosylation status of classical swine fever virus strain brescia e2 glycoprotein influences virulence in swine. Conference of Research Workers in Animal Disease (CRWAD) annual meeting, Chicago, IL, December 3-5, 2006, page 200.

Identification of a Novel Virulence Determinant Within the E2 Structural Glycoprotein of Classical Swine Fever Virus - (Abstract)

Risatti, G.R., Holinka, L.G., Carillo, C., Kutish, G.F., Lu, Z., Tulman, E.R., Fernandez-Sainz, I., Borca, M.V. 2006. Identification of a Novel Virulence Determinant Within the E2 Structural Glycoprotein of Classical Swine Fever Virus. Conference of Research Workers in Animal Disease (CRWAD) annual meeting, Chicago, IL, December 3-5, 2006, page 201.

Mutations in the Carboxi Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus is Responsible for Viral Attenuation in Swine - (Abstract)

Risatti, G.R., Holinka, L.G., Fernandez-Sainz, I., Carillo, C., Kutish, G.F., Lu, Z., Tulman, E.R., Rock, D.L., Borca, M.V. Mutations in the Carboxi Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus is Responsible for Viral Attenuation in Swine. Conference of Research Workers in Animal Disease (CRWAD) annual meeting, Chicago, IL, December 3-5, 2006, page 138.

N-LINKED GLYCOSYLATION STATUS OF CLASSICAL SWINE FEVER VIRUS STRAIN BRECIA E2 GLYCOPROTEIN INFLUENCES VIRULENCE IN SWINE - (Peer Reviewed Journal)

Rissatti, G., Holinka, L.G., Fernandez-Sainz, I., Carrillo, C., Lu, Z., Borca, M.V. 2007. N-linked glycosylation status of classical swine fever virus strain brecia e2 glycoprotein influences virulence in swine. Journal of Virology. Vol. 81, No.2, P. 924-933.

MUTATION OF E1 GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS AFFECTS VIRAL VIRULENCE IN SWINE - (Peer Reviewed Journal)

Risatti, G.R., Holinka, L.G., Lu, Z., Kutish, G.F., Tulman, E.R., French, R.A., Sur, J.H., Rock, D.L., Borca, M.V. 2005 Mutation of E1 Glycoprotein of Classical Swine Fever Virus Affects Viral Virulence in Swine. Journal of Virology 343: 116-127.

CHIMERIC CLASSICAL SWINE FEVER VIRUS CONTAINING THE E2 GENE FROM A VACCINE STRAIN IN THE GENETIC BACKGROUND OF STRAIN BRESCIA INDUCES SOLID EARLY PROTECTION IN SWINE - (Abstract)

Risatti, G.R., Holinka, L.G., Lu, Z., Kutish, G.F., Rock, D.L., Borca, M.V. 2005. Chimeric Classical Swine Fever Virus Containing the E2 Gene from a Vaccine Strain in the Genetic Background of Strain Brescia Induces Solid Early Protection in Swine. 6th Pestivirus Symposium, Thun, Switzerland.Page 89, P5-10

GENETIC AND PHENOTYPIC VARIATION OF FMDV DURING SERIAL PASSAGES IN PIGS - (Abstract)

Carrillo, C., Lu, Z., Tulman, E., Borca, M.V., Vagnozzi, A., Kutish, G.F., Rock, D.L. 2005. Genetic and Phenotypic Variation of FMDV During Serial Passages in Pigs. European Study Group on the Molecular Biology of Picornaviruses. P. E06.

NOVEL TOOLS TO PREVENT AND ERADICATE FOREIGN ANIMAL DISEASES - (Abstract)

Rodriguez, L.L., Baxt, B., Rieder, A.E., Golde, W.T., Borca, M.V., Grubman, M.J. 2005. Novel tools to prevent and eradicate foreign animal diseases. American Society for Microbiology Biodefense Meeting. p 41.

PRECLINICAL DIAGNOSIS OF AFRICAN SWINE FEVER IN CONTACT EXPOSED SWINE BY A PORTABLE REAL-TIME PCR ASSAY - (Proceedings/Symposium)

Zsak, L., Borca, M.V., Risatti, G.R., French, R.A., Lu, Z., Kutish, G.F., Neilan, J.G., Callahan, J.D., Nelson, W.M., Rock, D.L. 2004. Preclinical diagnosis of african swine fever in contact exposed swine by a portable real-time pcr assay [abstract]. Conference Research Workers Disease Meeting. p. 87.

THE E2 GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS AFFECTS VIRAL VIRULENCE IN SWINE - (Peer Reviewed Journal)

Risatti, G.R., Borca, M.V., Kutish, G.F., Lu, Z., Holinka, L.G., French, R., Tulman, E., Rock, D.L. 2005 The E2 Glycoprotein of Classical Swine Fever Virus Affects Viral Virulence in Swine. Journal of Virology.79: 3787-3796.

A 19MER PEPTIDE INSERTION IN THE E1 GLYCOPROTEIN OF CLASSICAL SWINE FEVER FEVER VIRUS AFFECTS VIRAL VIRULENCE IN SWINE - (Proceedings/Symposium)

Risatti, G.R., Kutish, G.F., Lu, Z., Holinka, L.G., French, R.A., Sur, J.H., Rock, D.L., Borca, M.V. 2004. A 19mer Peptide Insertion in the E1 Glycoprotein of Classical Swine Fever Virus Affects Viral Virulence in Swine. Conference of Research Worksers in Animal Disese Meeting. P.195

THE E2 GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS IS A VIRULENCE DETERMINANT IN SWINE - (Proceedings/Symposium)

Risatti, G.R., Kutish, G.F., Lu, Z., Holinka, L.G., French, R.A., Tulman, E., Rock, D.L., Borca, M.V. 2004. The E2 Glycoprotein of Classical Swine Fever Virus is a Virulence Determinant in Swine. Conference of Research Worksers in Animal Disese Meeting. P. 196.

DIAGNOSTIC EVALUATION OF A PORTABLE REAL-TIME REVERSE TRANSCRIPTASE PCR ASSAY FOR THE DETECTION OF CLASSICAL SWINE FEVER. - (Proceedings/Symposium)

Risatti, G.R., Holinka, L.G., Lu, Z., Kutish, G.F., Callahan, J.D., Nelson, W.N., Tio, B.E., Borca, M.V. 2004. Diagnostic Evaluation of a Portable Real-Time Reverse Transcriptase PCR Assay for the Detection of Classical Swine Fever. Conference of Research Worksers in Animal Disese Meeting. P 194.

PRECLINICAL DIAGNOSIS OF AFRICAN SWINE FEVER IN CONTACT EXPOSED SWINE BY A PORTABLE REAL-TIME PCR ASSAY - (Peer Reviewed Journal) - (06-Sep-04)

PRECLINICAL DIAGNOSIS OF AFRICAN SWINE FEVER IN CONTACT EXPOSED SWINE BY A PORTABLE REAL-TIME PCR ASSAY - (Peer Reviewed Journal)

Zsak, L., Borca, M.V., Risatti, G.R., French, R., Lu, Z., Kutish, G.F., Neilan, J.G., Callahan, J.D., Nelson, W.M., Rock, D.L. 2005. Preclinical Diagnosis of African Swine Fever in Contact Exposed Swine by a Portable Real-time PCR Assay. Journal of Clinical Microbiology. 43 (1):112-119.

DIAGNOSTIC EVALUATION OF A PORTABLE REAL-TIME REVERSE TRANSCRIPTASE PCR ASSAY FOR THE DETECTION OF CLASSICAL SWINE FEVER - (Peer Reviewed Journal)

Risatti, G.R., Holinka, L.G., Lu, Z., Kutish, G.F., Callahan, J.D., Nelson, W.M., Brea, T.E., Borca, M.V. 2005. Diagnostic Evaluation of a Portable Real-Time Reverse transcriptase PCR Assay for the Detection of Classical Swine Fever. Journal of Clinical Microbiology. 43 (1): 468-471.

DEVELOPMENT OF TRANSGENIC ALFALFA PLANTS CONTAINING THE FOOT AND MOUTH DISEASE VIRUS STRUCTURAL POLYPROTEIN GENE P1 AND ITS UTILIZATION AS AN EXPERIMENTAL IMMUNOGEN - (Peer Reviewed Journal)

Dus Santos, M., Carrillo, C., Ardila, F., Rios, R., Franzone, M., Piccone, M.E., Wigdorovitz, A., Borca, M.V. 2005. Development of Transgenic Alfalfa Plants Containing the Foot-and-Mouth Disease Virus Structural Polyprotein Gene p1 and Its Utilization as an Experimental Immunogen. Vaccine.23(15): 1838-43.

PRESERVED ANTIGENICITY OF HIV-1 P24 PRODUCED AND PURIFIED IN HIGH YIELDS FROM PLANTS INOCULATED WITH TOBACCO MOSAIC VIRUS (TMV)-DERIVED VECTOR - (Peer Reviewed Journal)

Perez, D., Brayfield, B., Phiri, S., Borca, M.V., Woods, C., Morris, T. 2004. Preserved antigenicity of hiv-1 p24 produced and purified in high yields from plants inoculated with tobacco mosaic virus (tmv)-derived vector.. Journal of Virological Methods. 121 (2004) 201-208.

PASSIVE PROTECTION TO BOVINE ROTAVIRUS (BRV) INFECTION INDUCED BY A BRV VP8* PRODUCED IN PLANTS USING A TMV-BASED VECTOR - (Peer Reviewed Journal)

Perez Filgueira, D., Mozgovoj, M., Wigdorovitz, A., Dus Santos, M., Parreno, V., Trono, K., Fernandez, F., Carrillo, C., Badiuk, L., Morris, T., Borca, M.V. 2004. Passive protection to bovine rotavirus (brv) infection induced by a brv vp8* produced in plants using a tmv-based vector.. Archives of Virology. 149:2337-2348.

A 19MER PEPTIDE INSERTION IN THE E1 GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS AFFECTS VIRAL VIRULENCE IN SWINE - (Proceedings/Symposium)

Borca, M.V., Risatti, G.R., Kutish, G.F., Lu, Z., Holinka, L.G., French, R.A., Sur, J., Rock, D.L. 2004. A 19mer peptide insertion in the e1 glycoprotein of classical swine fever virus affects viral virulence in swine [abstract]. Positive Strand RNA Virus International Conference Proceedings. p. 109.

THE E2 GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS AFFECTS VIRAL VIRULENCE IN SWINE - (Proceedings/Symposium)

Risatti, G.R., Kutish, G.F., Lu, Z., Holinka, L.G., French, R.A., Borca, M.V., Rock, D.L. 2004. The e2 glycoprotein of classical swine fever virus affects viral virulence in swine [abstract]. Positive Strand RNA Virus International Conference Proceedings. P. 109.

PROTECTIVE LACTOGENIC IMMUNITY CONFERRED BY AN EDIBLE PEPTIDE VACCINE TO BOVINE ROTAVIRUS PRODUCED IN TRANSGENIC PLANTS - (Peer Reviewed Journal)

Wigdorovitz, A., Mozgovoj, M., Dus Santos, M.J., Parreno, V., Perez-Filgueira, D.M., Trono, K.G., Rios, R.D., Carrillo, C., Escribano, J.M., Borca, M.V. 2004. Protective lactogenic immunity conferred by an edible peptide vaccine to bovine rotavirus produced in transgenic plants. Journal of General Virology. 85:1825-1832.

BOVINE HERPES VIRUS G-D PROTEIN PRODUCED IN PLANTS USING A RECOMBINANT TOBACCO MOSAIC VIRUS (TMV) VECTOR POSSESSES AUTHENTIC ANTIGENICITY - (Peer Reviewed Journal)

Perez Filgueira, D.M., Zamorano, P.I., Dominguez, M.G., Taboga, O., Del Medico Zajac, M.P., Puntel, M., Romera, S.A., Morris, T.J., Borca, M.V., Sadir, A.M. 2003. Bovine herpes virus g-d protein produced in plants using a recombinant tobacco mosaic virus (tmv) vector possesses authentic antigenicity. Vaccine. 21(27-30):4201-4209.

RAPID DETECTION OF CLASSICAL SWINE FEVER VIRUS BY A PORTABLE REAL-TIME REVERSE TRANSCRIPTASE PCR ASSAY - (Peer Reviewed Journal) - (03-Nov-02)

RAPID DETECTION OF CLASSICAL SWINE FEVER VIRUS USING A PORTABLE REAL TIME RT-PCR ASSAY - (Peer Reviewed Journal) - (22-Sep-02)