Marx PA, Lu CY, Makuwa M, Georges-Courbot MC, Telfer P, Dubreuil G, Chen Z, Smith SM, Gao F, Hahn BH; Conference on Retroviruses and Opportunistic Infections.
Program Abstr 5th Conf Retrovir Oppor Infect Conf Retrovir Oppor Infect 5th 1998 Chic Ill. 1998 Feb 1-5; 5th: 185 (abstract no. 557).
Aaron Diamond AIDS Research Center, New York, NY.
A serosurvey for simian immunodeficiency virus (SIV) antibody in pet monkeys in Gabon found a positive red-capped mangabey (RCM), C. t. torquatus. A virus, SIVrcm, was isolated by co-cultivation of its peripheral blood mononuclear cells (PBMC) with primary human PBMC. SIVrcm also grew by co-cultivation of CD8-depleted RCM PBMC with Molt 4 clone 8 cells. A clone, 8.4, was obtained that was 100% infected, had no cytopathology and continuously produced virus. Two rhesus macaques were infected with SIVrcm and showed declines in CD4 cell numbers. To determine the evolutionary origins of SIVrcm, subgenomic pol (475bp) and gag (954 bp) gene fragments were amplified and sequenced. The position of SIVrcm relative to other SIV lineages showed discordance in the phylogenetic positions of the two genomic regions. In trees derived from partial gag sequences, SIVrcm clustered independently from all other HIV and SIV strains, consistent with a new lentiviral lineage. However, in trees derived from pol sequences representing integrase, SIVrcm clustered with the HIV-1/SIVcpz lineage. SIVrcm is the first mangabey virus found with significant homology to HIV-1, but it may be mosaic, possibly the result of a recombination involving divergent lentiviruses in the distant past.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Animals
- Animals, Domestic
- Base Sequence
- CD4-Positive T-Lymphocytes
- Cercocebus
- Cercocebus atys
- Communicable Diseases
- Gabon
- Genes, gag
- Genes, pol
- HIV Infections
- HIV-1
- Humans
- Lentivirus
- Macaca mulatta
- Simian Acquired Immunodeficiency Syndrome
- Simian immunodeficiency virus
- genetics
Other ID:
UI: 102236143
From Meeting Abstracts