Bibliographic Citation
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| Title | Acrolein stimulates eicosanoid release from bovine airway epithelial cells |
| Creator/Author | Doupnik, C.A. ; Leikauf, G.D. (Univ. of Cincinnati College of Medicine, OH (USA)) |
| Publication Date | 1990 Oct 01 |
| OSTI Identifier | OSTI ID: 6267622 |
| Other Number(s) | ISSN0002-9513; CODEN: AJPHA |
| Resource Type | Journal Article |
| Resource Relation | American Journal of Physiology ; Vol/Issue: 259:4 Pt 1 |
| Subject | 560300 -- Chemicals Metabolism & Toxicology ;550501 -- Metabolism-- Tracer Techniques; ACROLEIN-- BIOLOGICAL EFFECTS;ARACHIDONIC ACID-- METABOLISM;EICOSANOIC ACID-- BIOSYNTHESIS;RESPIRATORY SYSTEM DISEASES-- PATHOGENESIS; CATTLE;CELL CULTURES;DOSE-RESPONSE RELATIONSHIPS;ENZYME ACTIVITY;EPITHELIUM;LACTATE DEHYDROGENASE;LIQUID COLUMN CHROMATOGRAPHY;PROSTAGLANDINS;TRACER TECHNIQUES;TRITIUM COMPOUNDS |
| Related Subject | ALDEHYDES;ANIMAL TISSUES;ANIMALS;BODY;CARBOXYLIC ACIDS;CHROMATOGRAPHY;DISEASES;DOMESTIC ANIMALS;ENZYMES;HEMIACETAL DEHYDROGENASES;HYDROGEN COMPOUNDS;ISOTOPE APPLICATIONS;MAMMALS;MONOCARBOXYLIC ACIDS;ORGANIC ACIDS;ORGANIC COMPOUNDS;OXIDOREDUCTASES;RUMINANTS;SEPARATION PROCESSES;SYNTHESIS;TISSUES;VERTEBRATES |
| Description/Abstract | Injury to the airway mucosa after exposure to environmental irritants is associated with pulmonary inflammation and bronchial hyperresponsiveness.^To better understand the relationships between mediator release and airway epithelial cell injury during irritant exposures, we studied the effects of acrolein, a low-molecular-weight aldehyde found in cigarette smoke, on arachidonic acid metabolism in cultured bovine tracheal epithelial cells.^Confluent airway epithelial cell monolayers, prelabeled with (3H)arachidonic acid, released significant levels of 3H activity when exposed (20 min) to 100 microM acrolein.^(3H)arachidonic acid products were resolved using reverse-phase high-performance liquid chromatography.^Under control conditions the released 3H activity coeluted predominantly with the cyclooxygenase product, prostaglandin (PG) E2.^After exposure to acrolein, significant peaks in 3H activity coeluted with the lipoxygenase products 12-hydroxyeicosatetraenoic acid (HETE) and 15-HETE, as well as with PGE2, PGF2 alpha, and 6-keto-PGF1 alpha.^Dose-response relationships for acrolein-induced release of immunoreactive PGF2 alpha and PGE2 from unlabeled epithelial monolayers demonstrated 30 microM acrolein as the threshold dose, with 100 microM acrolein inducing nearly a fivefold increase in both PGF2 alpha and PGE2.^Cellular viability after exposure to 100 microM acrolein, determined by released lactate dehydrogenase activity, was not affected until exposure periods were greater than or equal to 2 h. These results implicate the airway epithelial cell as a possible source of eicosanoids after exposure to acrolein. |
| Country of Publication | United States |
| Language | English |
| Format | Pages: L222-L229 |
| System Entry Date | 2001 May 13 |
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