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PSWMRU SOP
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1 - Table of Contents
2 - Adding Acid to Water
3 - Agarose Gel
4 - Autoclave
5 - Burning Field Crop Land
6 - Chloroform Phosgene
7 - Cold Room - Rock Springs
8 - Cold Room - Main Building
9 - Cold Room - Tower Road
10 - Desiccant
11 - Ethidium Bromide Spill Cleanup
12 - Forklift & Refresher Training
13 - Formaldehyde - Acetic Acid - Alcohol SOP
14 - Gloves & Specific Chemicals
15 - Greenhouse Etiquette
16 - Iron Oxide-Impregnated Paper Strip Method
17 - Kjeldhal Method
18 - Lyme Disease
19 - Mehlich Phosphate Analysis
20 - Material Safety Data Sheet (MSDS)
21 - Olsen Reagent: (Sodium Bicarbonate) SOP
22 - Polymerase Chain Reaction (PCR)
23 - Recycling Effort
24 - Rodents and Hanta Virus
25 - Root Ash Procedure
26 - Sharps, Broken Glassware, Needles
27 - Shop Equipment
28 - UV Light Use
29 - Welding
30 - Winter Safety
31 - Adding Water to Acid II
Adding Acid to Water

Never add Water to Acid, an extreme exothermic chemical reaction will occur.

1. Fill a container with ice from the ice machine in the growth chamber room in the basement.
2. Place the ice container into the sink.
3. Fill the ice container with cold water.
4. Place the Pyrex container of water into the ice bath.
5. Make sure the level of the ice bath does not cause the container to tip.
6. Wear gloves.
7. Gradually pour acid liquid into the Pyrex container.
8. Swirl container in ice bath to cool the chemical reaction.
9. Continue to pour acid slowly, allowing solution to cool as needed.

Alternate Method

1.  Place a secondary container into the sink, fill it with cold tap water.
2.  Place the Pyrex container of water into the container of water.
3.  Make sure the level of the cold water bath does not cause the Pyrex container to tip.
4.  Run tap water continuously into the secondary container to cool the reaction.
5.  Wear gloves.
6.  Follow from # 6 in the above directions.

17 March 1999

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Last Modified: 12/24/2008
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