Pieniazek D, Luo CC, Kalish M, LaTorre ND, Janini LM, Schochetman G, Rayfield M; National Conference on Human Retroviruses and Related Infections.
Program Abstr First Natl Conf Hum Retrovir Relat Infect Natl Conf Hum Retrovir Relat Infect 1st 1993 Wash DC. 1993 Dec 12-16; 105.
CDC, Atlanta, GA.
Objective: To conduct comparative phylogenetic analysis based on gag, pol and env genes of geographically distinct HIV-1 strains. Methods: HIV-1 proviral DNA from more than 90 isolates collected from countries in Asia, Africa and the Americas was PCR-amplified using nested primers flanking the p24 gag region, protease gene and V3 domain. The amplified products were endonuclease-digested for preliminary typing, and 50 genetically diverse isolates were selected for sequencing and phylogenetic analysis using maximum likelihood and distance matrix methods. Results: (i) Phylogenetic analysis of the three HIV-1 gene regions indicates the presence of at least three predominant ancestral branches that, through subsequent splitting led to the major genetic subgroups of HIV-1 recognized today. (ii) With the exception of North American HIV-1 strains, isolates from South America, Africa, and Asia have a broad spectrum of genetic diversity; the isolates are dispersed across the branches of phylogenetic trees constructed from previously published sequences. (iii) At the genotypic level, subtype group assignments can vary when comparing variable and conserved domains. Conclusions: PCR analysis of gag, pol and env regions in HIV-1 genome demonstrated that all three ancestral branches are viable and could be used as markers to survey local epidemics in Europe, Asia, Africa, and the Americas.
Publication Types:
Keywords:
- Africa
- Americas
- Asia
- Base Sequence
- Europe
- Genes, env
- Genes, gag
- Genes, pol
- Genotype
- HIV-1
- Polymerase Chain Reaction
- South America
- Variation (Genetics)
- genetics
- reverse transcriptase, Human immunodeficiency virus 1
Other ID:
UI: 102214243
From Meeting Abstracts