| Gut. 1994 April; 35(4): 483–489. | PMCID: PMC1374796 |
Lactobacillus acidophilus LA 1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria. M F Bernet, D Brassart, J R Neeser, and A L Servin Département de Microbiologie, UFR Sciences Pharmaceutiques, Paris, France. Abstract Four human Lactobacillus acidophilus strains were tested for their ability to adhere onto human enterocyte like Caco-2 cells in culture. The LA 1 strain exhibited a high calcium independent adhesive property. This adhesion onto Caco-2 cells required a proteinaceous adhesion promoting factor, which was present in the spent bacterial broth culture supernatant. LA 1 strain also strongly bound to the mucus secreted by the homogeneous cultured human goblet cell line HT29-MTX. The inhibitory effect of LA 1 organisms against Caco-2 cell adhesion and cell invasion by a large variety of diarrhoeagenic bacteria was investigated. As a result, the following dose dependent inhibitions were obtained: (a) against the cell association of enterotoxigenic, diffusely adhering and enteropathogenic Escherichia coli, and Salmonella typhimurium; (b) against the cell invasion by enteropathogenic Escherichia coli, Yersinia pseudotuberculosis, and Salmonella typhimurium. Incubations of L acidophilus LA 1 before and together with enterovirulent E coli were more effective than incubation after infection by E coli. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.8M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. Images in this article Click on the image to see a larger version. These references are in PubMed. This may not be the complete list of references from this article. - Kleeman, EG; Klaenhammer, TR. Adherence of Lactobacillus species to human fetal intestinal cells. J Dairy Sci. 1982 Nov;65(11):2063–2069. [PubMed]
- Coconnier, MH; Klaenhammer, TR; Kernéis, S; Bernet, MF; Servin, AL. Protein-mediated adhesion of Lactobacillus acidophilus BG2FO4 on human enterocyte and mucus-secreting cell lines in culture. Appl Environ Microbiol. 1992 Jun;58(6):2034–2039. [PubMed]
- Chauvière, G; Coconnier, MH; Kernéis, S; Fourniat, J; Servin, AL. Adhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cells. J Gen Microbiol. 1992 Aug;138 Pt 8:1689–1696. [PubMed]
- Reid, G; Bruce, AW; McGroarty, JA; Cheng, KJ; Costerton, JW. Is there a role for lactobacilli in prevention of urogenital and intestinal infections? Clin Microbiol Rev. 1990 Oct;3(4):335–344. [PubMed]
- Fogh, J; Fogh, JM; Orfeo, T. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst. 1977 Jul;59(1):221–226. [PubMed]
- Louvard, D; Kedinger, M; Hauri, HP. The differentiating intestinal epithelial cell: establishment and maintenance of functions through interactions between cellular structures. Annu Rev Cell Biol. 1992;8:157–195. [PubMed]
- Aubel, D; Darfeuille-Michaud, A; Joly, B. New adhesive factor (antigen 8786) on a human enterotoxigenic Escherichia coli O117:H4 strain isolated in Africa. Infect Immun. 1991 Apr;59(4):1290–1299. [PubMed]
- Darfeuille-Michaud, A; Aubel, D; Chauviere, G; Rich, C; Bourges, M; Servin, A; Joly, B. Adhesion of enterotoxigenic Escherichia coli to the human colon carcinoma cell line Caco-2 in culture. Infect Immun. 1990 Apr;58(4):893–902. [PubMed]
- Kerneis, S; Bilge, SS; Fourel, V; Chauviere, G; Coconnier, MH; Servin, AL. Use of purified F1845 fimbrial adhesin to study localization and expression of receptors for diffusely adhering Escherichia coli during enterocytic differentiation of human colon carcinoma cell lines HT-29 and Caco-2 in culture. Infect Immun. 1991 Nov;59(11):4013–4018. [PubMed]
- Kernéis, S; Chauvière, G; Darfeuille-Michaud, A; Aubel, D; Coconnier, MH; Joly, B; Servin, AL. Expression of receptors for enterotoxigenic Escherichia coli during enterocytic differentiation of human polarized intestinal epithelial cells in culture. Infect Immun. 1992 Jul;60(7):2572–2580. [PubMed]
- Finlay, BB; Falkow, S. Salmonella interactions with polarized human intestinal Caco-2 epithelial cells. J Infect Dis. 1990 Nov;162(5):1096–1106. [PubMed]
- Francis, CL; Jerse, AE; Kaper, JB; Falkow, S. Characterization of interactions of enteropathogenic Escherichia coli O127:H6 with mammalian cells in vitro. J Infect Dis. 1991 Oct;164(4):693–703. [PubMed]
- Gaillard, JL; Berche, P; Mounier, J; Richard, S; Sansonetti, P. In vitro model of penetration and intracellular growth of Listeria monocytogenes in the human enterocyte-like cell line Caco-2. Infect Immun. 1987 Nov;55(11):2822–2829. [PubMed]
- Mounier, J; Vasselon, T; Hellio, R; Lesourd, M; Sansonetti, PJ. Shigella flexneri enters human colonic Caco-2 epithelial cells through the basolateral pole. Infect Immun. 1992 Jan;60(1):237–248. [PubMed]
- Svensson, L; Finlay, BB; Bass, D; von Bonsdorff, CH; Greenberg, HB. Symmetric infection of rotavirus on polarized human intestinal epithelial (Caco-2) cells. J Virol. 1991 Aug;65(8):4190–4197. [PubMed]
- Okhuysen, PC; Ericsson, CD. Travelers' diarrhea. Prevention and treatment. Med Clin North Am. 1992 Nov;76(6):1357–1373. [PubMed]
- Evans, DG; Evans, DJ, Jr; Clegg, S; Pauley, JA. Purification and characterization of the CFA/I antigen of enterotoxigenic Escherichia coli. Infect Immun. 1979 Aug;25(2):738–748. [PubMed]
- Bilge, SS; Clausen, CR; Lau, W; Moseley, SL. Molecular characterization of a fimbrial adhesin, F1845, mediating diffuse adherence of diarrhea-associated Escherichia coli to HEp-2 cells. J Bacteriol. 1989 Aug;171(8):4281–4289. [PubMed]
- Girón, JA; Jones, T; Millán-Velasco, F; Castro-Muñoz, E; Zárate, L; Fry, J; Frankel, G; Moseley, SL; Baudry, B; Kaper, JB, et al. Diffuse-adhering Escherichia coli (DAEC) as a putative cause of diarrhea in Mayan children in Mexico. J Infect Dis. 1991 Mar;163(3):507–513. [PubMed]
- Isberg, RR; Voorhis, DL; Falkow, S. Identification of invasin: a protein that allows enteric bacteria to penetrate cultured mammalian cells. Cell. 1987 Aug 28;50(5):769–778. [PubMed]
- Lesuffleur, T; Barbat, A; Dussaulx, E; Zweibaum, A. Growth adaptation to methotrexate of HT-29 human colon carcinoma cells is associated with their ability to differentiate into columnar absorptive and mucus-secreting cells. Cancer Res. 1990 Oct 1;50(19):6334–6343. [PubMed]
- Chauvière, G; Coconnier, MH; Kerneis, S; Darfeuille-Michaud, A; Joly, B; Servin, AL. Competitive exclusion of diarrheagenic Escherichia coli (ETEC) from human enterocyte-like Caco-2 cells by heat-killed Lactobacillus. FEMS Microbiol Lett. 1992 Mar 15;70(3):213–217. [PubMed]
- Conway, PL; Kjelleberg, S. Protein-mediated adhesion of Lactobacillus fermentum strain 737 to mouse stomach squamous epithelium. J Gen Microbiol. 1989 May;135(5):1175–1186. [PubMed]
- Neeser, JR; Chambaz, A; Golliard, M; Link-Amster, H; Fryder, V; Kolodziejczyk, E. Adhesion of colonization factor antigen II-positive enterotoxigenic Escherichia coli strains to human enterocytelike differentiated HT-29 cells: a basis for host-pathogen interactions in the gut. Infect Immun. 1989 Dec;57(12):3727–3734. [PubMed]
- Falkow, S; Isberg, RR; Portnoy, DA. The interaction of bacteria with mammalian cells. Annu Rev Cell Biol. 1992;8:333–363. [PubMed]
- Cravioto, A; Tello, A; Villafán, H; Ruiz, J; del Vedovo, S; Neeser, JR. Inhibition of localized adhesion of enteropathogenic Escherichia coli to HEp-2 cells by immunoglobulin and oligosaccharide fractions of human colostrum and breast milk. J Infect Dis. 1991 Jun;163(6):1247–1255. [PubMed]
- Reid, G; Cook, RL; Bruce, AW. Examination of strains of lactobacilli for properties that may influence bacterial interference in the urinary tract. J Urol. 1987 Aug;138(2):330–335. [PubMed]
- Gotteland, M; Pochart, P; Dabbech, M; Bisetti, N; Desjeux, JF. In vivo effect of yogurt on excretion of enteropathogen Escherichia coli RDEC-1 during acute diarrhea in the just-weaned rabbit. J Pediatr Gastroenterol Nutr. 1992 Apr;14(3):264–267. [PubMed]
- Conway, PL; Gorbach, SL; Goldin, BR. Survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells. J Dairy Sci. 1987 Jan;70(1):1–12. [PubMed]
- Goldin, BR; Gorbach, SL; Saxelin, M; Barakat, S; Gualtieri, L; Salminen, S. Survival of Lactobacillus species (strain GG) in human gastrointestinal tract. Dig Dis Sci. 1992 Jan;37(1):121–128. [PubMed]
- Isolauri, E; Juntunen, M; Rautanen, T; Sillanaukee, P; Koivula, T. A human Lactobacillus strain (Lactobacillus casei sp strain GG) promotes recovery from acute diarrhea in children. Pediatrics. 1991 Jul;88(1):90–97. [PubMed]
|
This article has been 
