Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Albacore  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Thunnus alalunga
FDA Seafood List Common Name: Albacore
FDA Seafood List Market Name: Tuna
Unique IEF Gel Code: 19960730gel01
Unique Web Code: alg02Srr
Tissue Aliquot Code: thunalal E96-002 #02a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 11
LANE TYPE: pI Markers
ACTUAL GEL LANE: 12
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5383.40.391434138.8784822.513.53.539.613.20.380035
24.5520318.40.43226953.180078.14.84.55202.94.80.376741
35.229126.40.68487144.7117111.97.25.2290.84.10.655318
45.8543839.70.70418845.2331513.385.85437.54.20.697276
56.5560054.30.41435664.0601310.36.26.55599.36.20.368167
66.8564258.20.41883253.578489.15.56.85641.95.10.394868
77.3572665.80.34270784.3028410.96.67.35723.97.50.323005
88.1586077.90.11469101.96183538.15860.6100.110306
98.4593484.60.099862371.221953.11.98.45934.37.20.0957387
108.6597588.30.12377840.9225692.31.48.65974.44.20.124537
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3109298.90.12840671.439633.62.29.31091.56.60.122992
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 12
LANE TYPE: Specimen
ACTUAL GEL LANE: 13
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
15.5938134.50.12896720.4755520.80.55.59380.92.10.125768
25.62138835.10.44477132.479894.12.75.621388.33.10.444625
35.714409370.041473170.4618890.80.55.714409.36.60.039521
46.1550946.10.11903530.57713810.66.15508.62.90.113638
56.39256551.10.10239540.7314751.20.86.3925644.30.0967232
66.43157451.90.078641530.4166020.70.56.431573.22.80.0838866
76.50959253.60.57736243.96256.64.36.509592.63.90.572382
86.55460254.50.83229146.4178310.776.554602.24.30.844233
96.62761255.40.14251420.5347080.90.66.627611.52.20.137833
106.75162956.90.66133744.280447.24.76.7516293.80.635653
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.88364858.60.17998920.74291.20.86.883647.92.40.175632
127.02167160.70.11561771.481472.51.67.021671.17.50.111451
137.14669262.60.11863751.101121.81.27.1466924.60.133715
147.22470563.80.93305146.7355511.37.37.224705.24.10.937152
157.46974667.50.43857642.716324.537.469745.53.50.4325
167.58976669.30.84247735.150378.65.67.589765.13.40.863296
178.46293784.80.5752581311.9882013.18.462938.213.10.514545
188.972103493.60.445998139.57741610.48.9721037.8130.415119
PeakCounter: 18
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 13
LANE TYPE: pI Markers
ACTUAL GEL LANE: 14
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5363.30.3851912413.86529.620.83.524.7240.325943
24.5520218.30.51113143.592037.75.44.55202.44.50.451689
35.229326.60.77060345.4832111.78.25.2292.64.20.731788
45.8543839.80.71269856.08352139.15.85436.64.80.719083
56.5560054.50.45573564.518439.66.86.55599.56.20.408026
66.8564058.10.4650853.872538.35.86.85640.35.10.428691
77.3572365.70.34507874.173948.96.37.357217.20.326479
88.1585777.80.115875111.973274.238.1585610.70.104531
98.4593284.70.11721171.4041132.18.45931.96.80.116801
108.6597188.20.15807441.217122.61.88.65971.34.40.155041
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3109499.40.074718140.6503851.419.31091.73.80.0974034
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 14
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 15
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5413.70.382064127.8132586.63.543.412.30.357601
24.5521219.20.34714773.8808943.34.55212.77.10.307594
35.230127.20.4869275.187765.34.45.2300.16.60.445729
45.57538334.70.096972630.4966190.50.45.5753822.70.103277
55.60739035.30.40245832.43042.52.15.607389.93.40.408105
65.694409370.0373280.4297010.40.45.694410.48.10.0301157
75.8544340.10.46743143.364193.42.85.8544240.468759
86.16651246.30.10451230.5122150.50.46.166511.72.80.101987
96.41756751.30.048479720.158410.20.16.417566.91.70.0511909
106.47257952.40.014215330.1546830.20.16.472575.52.60.0340245
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.5559653.90.49694354.018854.13.46.55596.34.70.484192
126.62360554.80.79755334.8734554.16.623605.33.50.795533
136.69661455.60.099224940.7091360.70.66.696613.23.90.101987
146.8563357.30.60358154.95795.14.26.85633.35.30.528679
156.94765058.80.15212730.7522480.80.66.947650.32.90.14657
167.07767360.90.086635530.4930930.50.47.077673.13.20.0871881
177.19769462.80.084156160.8499940.90.77.1976975.90.0812832
187.28270964.20.85095445.463625.64.67.282709.33.60.847154
197.3572165.20.15854251.632991.71.47.35718.54.70.197987
207.5174767.60.365332.2252.31.97.51746.53.40.369807
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.63376769.40.80555134.969295.14.27.6337673.40.81388
228.15851770.136189153.513793.638.15848.914.70.134848
238.4593784.80.588345108.213548.46.98.45937.110.20.455977
248.65972880.5368292421.134221.617.98.65975.4240.49683
258.978103393.50.358432138.42148.67.18.9781039130.364496
269.3109398.90.15797251.347191.41.19.31092.15.20.147017
PeakCounter: 26
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 15
LANE TYPE: Specimen
ACTUAL GEL LANE: 16
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
15.56537934.30.082678130.4744580.90.55.565378.33.10.0859081
25.601387350.37479332.164274.12.25.601387.53.20.384501
35.68240536.60.061163240.3716520.70.45.682404.83.70.0562244
46.14850845.90.081034330.4188180.80.46.148507.930.0784282
56.39356250.80.11688740.7215571.40.76.393561.43.70.109829
66.4357051.50.10789330.6030521.10.66.43569.53.20.106269
76.52559153.40.48321632.6528352.76.525590.13.40.436777
86.554597540.85588846.5303712.26.86.554597.74.20.87266
96.636608550.1068830.7248061.40.86.636606.52.80.148029
106.76462556.50.62787443.83957.246.764624.73.70.58703
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.88864358.10.16737720.6760251.30.76.888642.62.30.168414
127.011664600.140374102.072133.92.17.011668.710.40.112092
137.139686620.17761641.0835321.17.139686.74.10.150537
147.23370263.50.93203346.6513912.56.97.233702.73.80.982984
157.464741670.40726142.6533252.77.464740.83.80.398816
167.58476168.80.8435834.91979.25.17.584760.53.30.84738
178.43993284.30.60321399.0238716.99.38.439933.69.20.553378
188.974103293.30.388686127.8089314.68.18.9741033.912.50.353234
PeakCounter: 18
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 16
LANE TYPE: pI Markers
ACTUAL GEL LANE: 17
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5433.90.41832414.273830.220.93.534.6240.335553
24.5520718.70.49241553.487937.45.14.552074.50.436276
35.229426.60.73363445.2789411.27.75.2293.94.20.702836
45.8544239.90.80089845.4292511.585.85441.83.80.800526
56.5559753.90.44517574.808610.276.55595.66.90.394951
66.8564057.80.49321954.198378.96.26.85639.85.10.467276
77.3572365.30.34954774.2769196.37.35721.77.40.325027
88.1586077.70.114805112.057764.338.15859.610.60.109857
98.4593384.30.10541171.228392.61.88.45932.96.50.106513
108.6597287.80.15160741.147432.41.78.65972.24.40.147857
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3109298.60.12109751.123432.41.69.31091.95.40.117517
PeakCounter: 11
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 3.50 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 6.85 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 3.50 label for gel schematic
white spacer for gel schematic
pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
white spacer for gel schematic
pI 8.45 label for gel schematic
white spacer for gel schematic
pI 8.65 label for gel schematic
white spacer for gel schematic
pI 9.30 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 95
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 11
LANE TYPE: pI Markers
ACTUAL GEL LANE: 12
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 12
LANE TYPE: Specimen
ACTUAL GEL LANE: 13
PeakCount: 18 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 13
LANE TYPE: pI Markers
ACTUAL GEL LANE: 14
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 14
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 15
PeakCount: 26 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 15
LANE TYPE: Specimen
ACTUAL GEL LANE: 16
PeakCount: 18 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 16
LANE TYPE: pI Markers
ACTUAL GEL LANE: 17
PeakCount: 11 Return to Lane Navigator



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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln