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Document Number | 1301 |
|---|---|
Primary Title | Response of Clostridium acetobutylicum to the Presence of Mixed Extractants |
Author Name | Evans, P. J.;Wang, H. Y. |
Author Affiliation | Univ. of Michigan, Ann Arbor, MI |
Editor Name | Scott, C. D. |
Conference Date | 05/05/1987 |
Conference Name | 9th Symposium on Biotechnology for Fuels and ChemicalsBoulder, CO,5 May 1987 |
Published Date | 04/01/1988 |
Detailed Publish Date | Apr 1988 |
Title Source | Applied Biochemistry and Biotechnology |
Volume Number | 17 |
Page Range | 175-192 |
Page Count | 18 |
Document Type | JOURNAL ARTICLE |
Publisher Name | Humana Press, Clifton, NJ |
Abstract | The production of butanol by Clostridium acetobutylicum ATCC 4259 was used as a model system to study the use of mixed extractants. Mixtures of toxic extractants having high distribution coefficients for the product and nontoxic extractants having low distribution coefficients were used. Growth was possible in mixtures composed of 20 to 30% (v/v) decanol in hexadecane. The toxicity of extractants was determined, and the order of toxicity precedence was corn oil, hexadecane and oleyl alcohol, with corn oil being the most toxic. The critical aqueous decanol concentration for a monophasic fermentation is presented. The critical aqueous decanol concentration was independent of the critical decanol volume fraction for the biphasic fermentations with corn oil, hexadecane, tridecane and oleyl alcohol. The results supported the hypothesis that the aqueous concentration of decanol is the sole determinant of toxicity to growth of C. acetobutylicum in a nondispersed system. The nontoxic coextractant could decrease the aqueous concentration of the toxic coextractant. (35 ref) |
Copyright Status | Y - Copyrighted--no reprint permission, |
Document Owner | B |