Bibliographic Citation
Document | For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field. For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability. |
---|---|
Title | Fatty aldehyde dehydrogenases in Acinetobacter sp. strain HO1-N: role in hexadecane and hexadecanol metabolism |
Creator/Author | Singer, M.E. ; Finnerty, W.R. |
Publication Date | 1985 Dec 01 |
OSTI Identifier | OSTI ID: 5321688 |
Other Number(s) | CODEN: JOBAA |
Resource Type | Journal Article |
Resource Relation | J. Bacteriol. ; Vol/Issue: 164:3 |
Research Org | Univ. of Georgia, Athens |
Subject | 550201 -- Biochemistry-- Tracer Techniques ;550701 -- Microbiology-- Tracer Techniques; BACTERIA-- BIOCHEMICAL REACTION KINETICS;DECANOLS-- METABOLISM;HEXADECANE-- METABOLISM; ALDEHYDES;ENZYME INDUCTION;OXIDOREDUCTASES;TRITIUM COMPOUNDS |
Related Subject | ALCOHOLS;ALKANES;ENZYMES;GENE REGULATION;HYDROCARBONS;HYDROXY COMPOUNDS;KINETICS;LABELLED COMPOUNDS;MICROORGANISMS;ORGANIC COMPOUNDS;REACTION KINETICS |
Description/Abstract | The role of fatty aldehyde dehydrogenases (FALDHs) in hexadecane and hexadecanol metabolism was studied in Acinetobacter sp. strain HO1-N.^Two distinct FALDHs were demonstrated in Acinetobacter sp. strain HO1-N: (i) a membrane-bound, NADP-dependent FALDH activity induced 5-, 15-, and 9 fold by growth on hexadecanol, dodecyl aldehyde, and hexadecane, respectively, and (ii) a constitutive, NAD-dependent, membrane-localized FALDH.^Dodecyl aldehyde-negative mutants were isolated and grouped into two phenotypic classes based on growth: class 1 mutants were hexadecane and hexadecanol negative and class 2 mutants were hexadecane and hexadecanol positive.^Specific activity of NADP-dependent FALDH in Ald21 (class 1 mutant) was 85% lower than that of wild-type FALDH, while the specific activity of Ald24 (class 2 mutant) was 55% greater than that of wild-type FALDH.^Ald21R, a dodecyl aldehyde-positive revertant able to grow on hexadecane, hexadecanol, and dodecyl aldehyde, exhibited a 100% increase in the specific activity of the NADP-dependent FALDH.^This study provides genetic and physiological evidence for the role of fatty aldehyde as an essential metabolic intermediate and NADP-dependent FALDH as a key enzyme in the dissimilation of hexadecane, hexadecanol, and dodecyl aldehyde in Acinetobacter sp. strain HO1-N. |
Country of Publication | United States |
Language | English |
Format | Pages: 1011-1016 |
System Entry Date | 2001 May 13 |
Top |