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Title Fatty aldehyde dehydrogenases in Acinetobacter sp. strain HO1-N: role in hexadecane and hexadecanol metabolism
Creator/Author Singer, M.E. ; Finnerty, W.R.
Publication Date1985 Dec 01
OSTI IdentifierOSTI ID: 5321688
Other Number(s)CODEN: JOBAA
Resource TypeJournal Article
Resource RelationJ. Bacteriol. ; Vol/Issue: 164:3
Research OrgUniv. of Georgia, Athens
Subject550201 -- Biochemistry-- Tracer Techniques ;550701 -- Microbiology-- Tracer Techniques; BACTERIA-- BIOCHEMICAL REACTION KINETICS;DECANOLS-- METABOLISM;HEXADECANE-- METABOLISM; ALDEHYDES;ENZYME INDUCTION;OXIDOREDUCTASES;TRITIUM COMPOUNDS
Related SubjectALCOHOLS;ALKANES;ENZYMES;GENE REGULATION;HYDROCARBONS;HYDROXY COMPOUNDS;KINETICS;LABELLED COMPOUNDS;MICROORGANISMS;ORGANIC COMPOUNDS;REACTION KINETICS
Description/Abstract The role of fatty aldehyde dehydrogenases (FALDHs) in hexadecane and hexadecanol metabolism was studied in Acinetobacter sp. strain HO1-N.^Two distinct FALDHs were demonstrated in Acinetobacter sp. strain HO1-N: (i) a membrane-bound, NADP-dependent FALDH activity induced 5-, 15-, and 9 fold by growth on hexadecanol, dodecyl aldehyde, and hexadecane, respectively, and (ii) a constitutive, NAD-dependent, membrane-localized FALDH.^Dodecyl aldehyde-negative mutants were isolated and grouped into two phenotypic classes based on growth: class 1 mutants were hexadecane and hexadecanol negative and class 2 mutants were hexadecane and hexadecanol positive.^Specific activity of NADP-dependent FALDH in Ald21 (class 1 mutant) was 85% lower than that of wild-type FALDH, while the specific activity of Ald24 (class 2 mutant) was 55% greater than that of wild-type FALDH.^Ald21R, a dodecyl aldehyde-positive revertant able to grow on hexadecane, hexadecanol, and dodecyl aldehyde, exhibited a 100% increase in the specific activity of the NADP-dependent FALDH.^This study provides genetic and physiological evidence for the role of fatty aldehyde as an essential metabolic intermediate and NADP-dependent FALDH as a key enzyme in the dissimilation of hexadecane, hexadecanol, and dodecyl aldehyde in Acinetobacter sp. strain HO1-N.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 1011-1016
System Entry Date2001 May 13

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