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DOI 10.1016/j.taap.2006.03.006
Title Effect of lycopene and {beta}-carotene on peroxynitrite-mediated cellular modifications
Creator/Author Muzandu, Kaampwe [Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18, W9, Kita-Ku, Sapporo 060-0818 (Japan)] ; Ishizuka, Mayumi [Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18, W9, Kita-Ku, Sapporo 060-0818 (Japan)] ; Sakamoto, Kentaro Q. [Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18, W9, Kita-Ku, Sapporo 060-0818 (Japan)] ; Shaban, Zein [Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18, W9, Kita-Ku, Sapporo 060-0818 (Japan)] ; El Bohi, Khlood [Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18, W9, Kita-Ku, Sapporo 060-0818 (Japan)] ; Kazusaka, Akio [Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18, W9, Kita-Ku, Sapporo 060-0818 (Japan)] ; Fujita, Shoichi [Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18, W9, Kita-Ku, Sapporo 060-0818 (Japan)]. E-mail: fujita@vetmed.hokudai.ac.jp
Publication Date2006 Sep 15
OSTI IdentifierOSTI ID: 20850419
Other Number(s)Journal ID: ISSN 0041-008X; TXAPA9; TRN: US07R1681020881
Resource TypeJournal Article
Resource RelationJournal: Toxicology and Applied Pharmacology; Journal Volume: 215; Journal Issue: 3; Other Information: DOI: 10.1016/j.taap.2006.03.006; PII: S0041-008X(06)00104-9; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Subject60 APPLIED LIFE SCIENCES; CAROTENOIDS; DNA; DOSES; FIBROBLASTS; HAMSTERS; IN VIVO; INFLAMMATION; LUNGS; NEOPLASMS; NITRATION; NITRIC OXIDE; NITROGEN; PROTEINS; SCAVENGING; STRAND BREAKS; TYROSINE; URIC ACID
Description/Abstract Peroxynitrite formed by the reaction of superoxide and nitric oxide is a highly reactive species with a role in various pathological processes such as cancer, chronic inflammation, and cardiovascular and neurological diseases. In the present study, the effect of the carotenoids, lycopene and {beta}-carotene, on peroxynitrite-mediated modifications in plasmid DNA as well as cellular DNA and proteins were investigated. In pUC18 plasmid DNA, these carotenoids strongly inhibited DNA strand breaks caused by peroxynitrite generated from 3-morpholinosydnonimine (SIN-1). SIN-1 was also used to determine effects on DNA damage and protein tyrosine nitration in Chinese hamster lung fibroblasts. SIN-1 dose-dependently increased nitration of proteins in cells above basal levels as determined by Western blotting. This nitration was inhibited in the presence of the uric acid as well as lycopene. Physiological concentrations (0.31-10 {mu}M) of lycopene and {beta}-carotene also had protective effects on DNA damage, as measured by the comet assay. Lycopene significantly reduced DNA damage particularly, in the median range of concentrations (2.5 {mu}M). The protective effects of lycopene and {beta}-carotene could be due to their scavenging of reactive oxygen (ROS) and/or nitrogen species (RNS) as they reduce the amount of intracellular ROS/RNS produced following treatment with SIN-1 by as much as 47.5% and 42.4%, respectively. The results obtained in this study suggest that carotenoids may alleviate some of the deleterious effects of peroxynitrite and possibly other reactive nitrogen species as well in vivo.
Country of PublicationUnited States
LanguageEnglish
FormatSize: page(s) 330-340
System Entry Date2007 Apr 02

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