4/9/4 (Item 4 from file: 155)

04869521 85233349

Arachidonic acid facilitates experimental chronic osteomyelitis in rats.

Rissing JP; Buxton TB; Fisher J; Harris R; Shockley RK

Infect Immun ( UNITED STATES ) Jul 1985 , 49 (1) p141‑4

Arachidonic acid was used as a facilitating agent in experimental rat Staphylococcus aureus osteomyelitis and compared with the more commonly used agent, sodium morrhuate. The injection of arachidonic acid or sodium morrhuate and S. aureus into rat tibiae caused increased quantitative bacterial bone counts, gross bone pathology, roentgenographic changes, and weight loss. The doses required to produce these changes appeared to be lower for arachidonic acid.

Tags: Animal

Descriptors: *Arachidonic Acids‑‑Toxicity‑‑TO;

*Osteomyelitis‑‑Etiology‑‑ET ; Disease Models, Animal;

Osteomyelitis‑‑Pathology‑‑PA; Osteomyelitis ‑‑Radiography‑‑RA; Rats;

Sodium Morrhuate‑‑Toxicity‑‑TO; Staphylococcal

Infections‑‑Complications‑‑CO; Staphylococcus aureus‑‑Pathogenicity‑‑PY;

Tissue Culture

CAS Registry No.: 0 (Arachidonic Acids); 506‑32‑1 (Arachidonic Acid);

8031‑09‑2 (Sodium Morrhuate)

4/9/15 (Item 2 from file: 94)

00880819 JICST Accession Number: 89A0234525 File Segment: JICST‑E

Study on experimental osteomyelitis in mice.

YOKOYAMA TAKASHI (1)

Tokyo Ika Daigaku Zasshi (Journal of Tokyo Medical College) , 1989 ,

VOL.47,NO.1 , PAGE.91‑104 , FIG.6, TBL.10, REF.26

Abstract: Osteomyelitis, a representative refractory infectious disease in the field of orthopedics, is liable to develop into a prolonged and chronic disease. In order to investigate the pathogenesis of osteomyelitis, attempts have been made to produce experimental animal model of the purulent osteomyelitis, however ideal model has not been developed. The present paper reports that a mouse model of experimental osteomyelitis was established successfully by injection of Staphylococcus aureus with arachidonic acid as a sclerosing agent directly into the medullary cavity of the tibia of mice with a microsyringe. By injecting both 105.6 CFU of Staphylococcus aureus No.28 and 31.5ng of sodium salt of arachidonic acid, the optimal condition for the formation of osteomyelitis in this model, the localized purulent osteomyelitis which closely resemble the human disease radiologically and histologically was fully recognized. For the first 2 weeks, the mice received both of the agents showed a statistically higher amount of prostaglandin in the tibia compared with the group of the staphylococci alone. In the leukopenic mice due to cyclophosphamide, the osteomyelitis occured in 90% of the mice by an intramedullary inoculation of 104.2 CFU of Staphylococcus aureus without use of the sclerosing agent. The evaluation method of the osteomyelitis was contrived by the score expressing the lesion of the osteomyelitis numerically in terms of its radiological, bacteriological and histological appearance, and the relative severity which was calculated from each score of the three findings made it possible to evaluate the osteomyelitic lesion quantitatively. It was made possible to compare the rate of formation and the severity of the osteomyelitis among experimental groups with a high accuracy, especially through the three criteria for determining the formation of osteomyelitis, based on the soft X‑ray findings and the relative severity.

Descriptors: mouse (animal); osteomyelitis; Staphylococcus aureus;

experimental disease; X‑ray inspection; vitamin F; polyene; aliphatic

carboxylic acid; unsaturated carboxylic acid

Broader Descriptors: Myomorpha; Rodentia; Mammalia; Vertebrata; animal;

inflammation; disease; infectious disease; bone disease; bone and joint

disease; bone marrow disease; hematologic disease; Staphylococcus;

Micrococcaceae; bacterium; microorganism; model; radiographic inspection;

nondestructive inspection; inspection; fatty acid; carboxylic acid;

fat‑soluble vitamin; vitamin; olefin compound

Classification Codes: EJ10030R