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Actinobacillus actinomycetemcomitans Search Results

Record: 1 of 1  
MiniMap IGR0625 IGR0630 IGR0628 IGR0626 IGR0627 IGR0632 IGR0629 IGR0631 AA00965 pckA,ppc, - AA00964 hupA, - AA00972 AA00971 AA00967 AA00973 nudC,yjaD, - AA00969 uroD,hemE, - AA00970 ppx, - AA00962 AA00961 AA00965 pckA,ppc, - AA00964 hupA, - AA00972 AA00971 AA00967 AA00973 nudC,yjaD, - AA00969 uroD,hemE, - AA00970 ppx, - AA00962 AA00961 AA00965 pckA,ppc, - AA00964 AA00967 AA00973 nudC,yjaD, - AA00969 uroD,hemE, - AA00970 ppx, - AA00962 AA00961 hupA, - AA00972 AA00971


Gene ID:AA00964

DNA Molecule Name:
1  

GenBank ID:


Gene Name:
pckA  ppc  

Definition:
phosphoenolpyruvate carboxykinase/carboxylase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
674428

Gene Stop:
672815

Gene Length:
1614

Molecular Weight*:
59487

pI*:


Net Charge*:


EC:
4.1.1.49  

Functional Class:
Energy metabolism; Fermentation  
Energy metabolism; Glycolysis and gluconeogenesis  

Gene Ontology:
Biological process
  GO:0006094    gluconeogenesis

Molecular function
  GO:0004611    phosphoenolpyruvate carboxykinase activity
  GO:0004612    phosphoenolpyruvate carboxykinase (ATP) activity
  GO:0005524    ATP binding
  GO:0005525    GTP binding


Pathway: pathway table
Carbon fixation
Citrate cycle (TCA cycle)
Pyruvate metabolism

Secondary Evidence:
Medina,V., Pontarollo,R., Glaeske,D., Tabel,H. and Goldie,H.
Sequence of the pckA gene of Escherichia coli K-12: relevance
to genetic and allosteric regulation and homology of E. coli
phosphoenolpyruvate carboxykinase with the enzymes from
Trypanosoma brucei and Saccharomyces cerevisiae.
J. Bacteriol. 172(12): 7151-7156, 1990.
PubMed: 1701430.

Laivenieks,M., Vieille,C. and Zeikus,J.G.
Cloning, sequencing, and overexpression of the
Anaerobiospirillum succiniciproducens phosphoenolpyruvate
carboxykinase (pckA) gene. Appl. Environ. Microbiol. 63(6):
2273-2280, 1997.
PubMed: 9172347.

Velayudhan J, Kelly DJ.
Analysis of gluconeogenic and anaplerotic enzymes in
Campylobacter jejuni: an essential role for
phosphoenolpyruvate
carboxykinase.
Microbiology. 2002 Mar;148(Pt 3):685-94.
PMID: 11882702

Prost JF, Cozzone AJ.
Detection of an extended-10 element in the promoter region of
the pckA gene encoding phosphoenolpyruvate carboxykinase in
Escherichia coli.
Biochimie. 1999 Mar;81(3):197-200.
PMID: 10385000

Comment:


View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|42630363|ref|ZP_00155907.1|  COG1866: Phosphoenolpyruvate...  1033   0.0  
 gi|148827997|ref|YP_001292750.1|  phosphoenolpyruvate carbox...  1031   0.0  
 gi|145632168|ref|ZP_01787903.1|  phosphoenolpyruvate carboxy...  1031   0.0  
 gi|68249404|ref|YP_248516.1|  phosphoenolpyruvate carboxykin...  1031   0.0  
 gi|145630556|ref|ZP_01786336.1|  phosphoenolpyruvate carboxy...  1030   0.0  
 gi|16272750|ref|NP_438969.1|  phosphoenolpyruvate carboxykin...  1029   0.0  
 gi|148826546|ref|YP_001291299.1|  phosphoenolpyruvate carbox...  1029   0.0  
 gi|145634779|ref|ZP_01790487.1|  phosphoenolpyruvate carboxy...  1027   0.0  
 gi|15603407|ref|NP_246481.1|  phosphoenolpyruvate carboxykin...   984   0.0  
 gi|53728709|ref|ZP_00131803.2|  COG1866: Phosphoenolpyruvate...   984   0.0


InterPro Summary:  InterProScan

InterPro
IPR001272
Family
Phosphoenolpyruvate carboxykinase (ATP)
PIRSF006294 [8-538]T 0 PIRSF006294 Phosphoenolpyruvate carboxykinase [ATP] Phosphoenolpyruvate carboxykinase [ATP]
PF01293 [18-491]T 0 PF01293 PEPCK_ATP PEPCK_ATP
TIGR00224 [2-538]T 0 TIGR00224 pckA: phosphoenolpyruvate carboxykinase (AT pckA: phosphoenolpyruvate carboxykinase (AT
PS00532 [263-278]T 8e-5 PS00532 PEPCK_ATP PEPCK_ATP
InterPro
IPR008210
Domain
Phosphoenolpyruvate carboxykinase, N-terminal
PD004738 [10-225]T 1e-102 PD004738 PPCK_HAEIN_P43923; PPCK_HAEIN_P43923;
noIPR
unintegrated
unintegrated
G3DSA:3.40.449.10 [25-225]T 5.5e-81 G3DSA:3.40.449.10 no description no description
G3DSA:3.90.228.20 [226-538]T 3.3e-111 G3DSA:3.90.228.20 no description no description


COGS Summary:  COGS Search
BeTs to 9 clades of COG1866
COG name: Phosphoenolpyruvate carboxykinase (ATP)
Functional Class: C
The phylogenetic pattern of COG1866 is -----zy--d--b-efgh--uj----
Number of proteins in this genome belonging to this COG is
Blocks Summary:  Blocks Search

Significant hit (4.3e-232) to 8/8 blocks of the IPB001272 family, which is described as "Phosphoenolpyruvate carboxykinase (ATP)". Interpro entry for IPR001272.
    IPB001272A    48-82    1.7e-27
    IPB001272B    124-168    3.4e-29
    IPB001272C    199-222    1.4e-23
    IPB001272D    239-280    3.9e-39
    IPB001272E    319-342    9.6e-19
    IPB001272F    370-423    6.9e-49
    IPB001272G    428-444    5.3e-10
    IPB001272H    457-500    2.6e-27

 ProDom Summary:  Protein Domain Search

Residues 10 to 225 match (4e-103) PD:PD004738 which is described as PHOSPHOENOLPYRUVATE CARBOXYKINASE LYASE DECARBOXYLASE GLUCONEOGENESIS CARBOXYLASE PEPCK PEP COMPLETE ATP

Paralogs:  Local Blast Search

AA00964 has no significant similarity (blastp p-value < 1e-3) to any other gene in this genome.


Pfam Summary:  Pfam Search


 Top PDB Hits:


Gene Protein Sequence:
MTDVNDVVKELEAIGIHNVKEVVYNPSYEQLFEEETKPGLEGFEKGTVTT
TGAVAVDTGIFTGRSPKDKYIVLDDTTKDTVWWTSDAAKNDNKPMTQETW
ASLKDIVTKELSGKRLFVIDGFCGASEKDRIAVRIVTEVAWQAHFVKNMF
IRPTDAELKDFKPSFVVMNGSKCTNPNWKEQGLNSENFVAFNLTERIQLI
GGTWYGGEMKKGMFSMMNYFLPLKGVGAMHCSANVGKDGDVAVFFGLSGT
GKTTLSTDPKRELIGDDEHGWDDVGIFNFEGGCYAKTIHLSEENEPDIYR
AIRRDALLENVVVRADGSVDFDDGSKTENTRVSYPIYHIDNIVKPVSRAG
HATKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPT
PTFSACFGAAFLTLHPTQYAEVLVKRMQAAGAEAYLVNTGWNGTGKRISI
KDTRGIIDAILDGSIEKAQMSELPIFNLAIPKALPGVDPAILDPRDTYAD
KAQWEAKAKDLAGRFVKNFEKYATNAEGKALISAGPKA

Gene Nucleotide Sequence:  Sequence Viewer
ATGACAGATGTAAACGACGTAGTTAAAGAACTTGAGGCAATTGGTATTCA
TAATGTAAAAGAAGTGGTTTATAACCCAAGTTATGAACAACTTTTTGAAG
AAGAAACCAAACCGGGCTTGGAAGGTTTCGAAAAAGGCACCGTCACCACC
ACCGGCGCGGTCGCTGTGGATACCGGCATTTTTACCGGACGTTCTCCTAA
GGATAAATATATTGTTTTAGATGACACCACCAAAGACACCGTATGGTGGA
CTTCCGACGCGGCGAAAAACGACAATAAACCGATGACCCAAGAAACCTGG
GCAAGCTTAAAAGACATCGTCACCAAAGAGCTTTCCGGCAAACGCTTATT
CGTGATTGACGGTTTCTGCGGCGCCAGCGAAAAAGACCGTATTGCGGTGC
GTATCGTGACTGAAGTGGCATGGCAGGCGCATTTCGTGAAAAATATGTTT
ATTCGTCCGACCGACGCCGAATTAAAAGATTTCAAACCAAGTTTCGTGGT
GATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGA
ATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATC
GGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGAT
GAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTA
ACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACC
GGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGA
TGAACACGGTTGGGATGATGTGGGTATCTTCAACTTTGAAGGTGGTTGTT
ACGCGAAAACCATTCACCTTTCCGAAGAAAACGAACCGGATATTTATCGC
GCAATCCGTCGTGATGCCTTATTAGAAAACGTAGTGGTGCGTGCAGACGG
TTCAGTGGATTTCGATGACGGTTCCAAAACCGAAAACACCCGCGTTTCCT
ATCCGATTTATCACATCGATAACATCGTTAAACCGGTTTCCCGCGCAGGT
CACGCTACTAAAGTGATTTTCTTAACCGCTGACGCTTTCGGCGTGTTGCC
GCCGGTATCTAAATTGACACCGGAACAAACCAAATACTACTTCTTATCCG
GTTTCACTGCGAAATTAGCCGGTACCGAACGTGGTATTACCGAACCGACC
CCAACATTCTCCGCTTGTTTCGGTGCGGCATTCTTAACCTTACACCCAAC
CCAATATGCGGAAGTGTTGGTAAAACGTATGCAAGCGGCAGGTGCGGAAG
CCTATTTGGTGAACACCGGTTGGAACGGCACGGGCAAACGTATCTCCATT
AAAGATACGCGCGGTATTATCGACGCCATCTTAGACGGTTCTATCGAAAA
AGCGCAAATGAGCGAATTACCAATCTTCAATTTGGCAATTCCTAAGGCAT
TACCCGGCGTTGATCCGGCTATTTTAGATCCGCGTGATACTTATGCCGAT
AAAGCGCAATGGGAAGCCAAAGCGAAAGATCTTGCTGGTCGTTTTGTGAA
GAATTTCGAAAAATACGCTACCAATGCAGAAGGTAAAGCCTTAATTTCTG
CCGGGCCCAAAGCG

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