Trenti T, Trenti F, Mongiardo N, Pecorari M, Pietrosemoli P, Sternieri E; International Conference on AIDS.
Int Conf AIDS. 1993 Jun 6-11; 9: 503 (abstract no. PO-B30-2208).
Dept. of Toxicology and Clinical Pharmacology, University of Modena, Italy.
BACKGROUND: Reduced glutathione (GSH) and cysteine (Cys) play an important role in lymphocyte activation and in lymphocyte-mediated cytotoxicity since the activation of T cells require the action of oxidizing and reducing substances as GSH and Cys. It is known that GSH is decreased in HIV infected peripheral blood mononuclear cells (BPMc) and this deficiency may potentiate HIV replication. AIM: The aim of the work was to evaluate the potential role of exogenous administration of GSH in HIV patients to replenish the GSH depleted store as it is unclear if the administered GSH may enter the cells. RESULTS: GSH (Tationil, Boehringer Mannheim, Milano, Italy) was administered i.v. as a bolus 70 micrograms/kg to 10 HIV patients with a low GSH amount in PBMc (4.28 +/- 1.69 vs 6.12 +/- 1.75 nmol/10(6) cells in controls, P < 0.02). GSH measured in HIV PBMc patients 2 hr after the administration of GSH bolus was significantly higher than in basal condition and a much higher concentration was found in PBMc after 4 hr (6.93 +/- 1.45 at 2hr, 7.81 +/- 2.23 nmol/10(6) at 4hr, P < 0.01 and P < 0.005 respectively vs basal values). Cysteine concentration, a precursor in GSH biosynthesis, exhibits a similar pattern in relation to GSH administration showing a significant increase. CONCLUSION: The present data support the possible use of exogenous GSH administration in replenish the GSH and Cysteine store in PBMc of HIV patients when depleted.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Cysteine
- Glutathione
- HIV
- HIV Infections
- HIV Seropositivity
- HIV-2
- Humans
- Italy
- Sulfhydryl Compounds
- T-Lymphocytes
- organization & administration
Other ID:
UI: 102205210
From Meeting Abstracts