Joseph S. Wall

Biology Department, 463
Brookhaven National Laboratory
Upton, NY 11973-5000

tel: (631) 344-2912 / 3372
fax: (631) 344-3407
wall@bnl.gov

The Group:
Joseph S. Wall (631) 344-2912
Frank E. Kito (631) 344-3372
Beth Yu Lin (631) 344-3372
Martha M. Simon (631) 344-3372
 

Research Interests:

Mass mapping of unstained biological molecules with the scanning transmission electron microscope (STEM), particularly assemblies of complexes from subunits of known size and shape. Examples include: Alzheimer's filaments, viral capsids, annelid hemoglobins, hemocyanins, proteases, chaperonins, microtubule proteins, prions and various nucleic acid-protein complexes. Another research area is instrument development involving design and construction of an instrument for low-temperture, energy loss spectroscopy, and elemental mapping at low dose. This is being used to map phosphorus in nucleic acid-protein complexes, phosphorylated proteins and phospholipid structures.

He also is director of the Scanning Transmission Electron Microscope STEM Facility which currently hosts over 50 user projects per year from around the world. These projects focus on use of the STEM for mass measurements of single molecules, or of gold cluster labeling for identifying molecular sites. Wall represents BNL (along with Y. Zhu) on DOE's TEAM Project (Transmission Electron Aberration-corrected Electron Microscope) which seeks to develop instruments with 0.5Å resolution. He is active in the CFN (Center for Functional Nanomaterials) in demonstrating the capabilities of the state-of-the-art aberration-corrected instruments recently installed there.

References:
Kendall A., McDonald M., Bian W., Bowles T., Baumgarten S.C., Shi J., Stewart P.L., Bullitt E., Gore D., Irving T.C., Havens W.M., Ghabrial S.A., Wall J.S. and Stubbs G.
Structure of flexible filamentous plant viruses.
Journal of Virology, 82(19):9546-9554 (2008).  PubMed
Volkov V.V., Wall J. and Zhu Y.
Position-sensitive diffractive imaging in STEM by an automated chaining diffraction algorithm.
Ultramicroscopy, 108(8):741-749 (2008).  PubMed
Wall J.S., Simon M.N., Lin B.Y. and Vinogradov S.N.
Mass mapping of large globin complexes by scanning transmission electron microscopy.
Methods in Enzymology, Vol. 436: Globins and Other Nitric Oxide-Reactive Proteins, Part A, Chapter 27, pp. 487-501, Elsevier, Inc. (2008).  PubMed
Deptuch G., Besson A., Rehak P., Szelezniak M., Wall J., Winter M., Zhu Y.
Direct Electron Imaging in Electron Microscopy with Monolithic Active Pixel Sensors.
Ultramicroscopy, 107(8):674-684 (2007).  PubMed
Jiang Z.G., Simon M.N., Wall J.S., and McKnight C.J.
Structural analysis of reconstituted lipoproteins containing the N-terminal domain of apolipoprotein B.
Biophysical J., 92(11):4097-4108 (2007).  PubMed
Ohi M.D., Feoktistova A., Ren L., Yip C., Cheng Y., Chen J.S., Yoon H.J., Wall J.S., Huang Z., Penczek P.A., Gould K.L., and Walz T.
Structural organization of the anaphase-promoting complex bound to the mitotic activator slp1.
Molecular Cell, 28(5):871-85 (2007).  PubMed
Ohi M.D., Ren L., Wall J.S., Gould K.L., and Walz T.
Structural characterization of the fission yeast U5.U2/U6 spliceosome complex.
Proc National Acad of Sci USA, 104(9):3195-3200 (2007).  PubMed
Sen A., Baxa U., Simon M.N., Wall J.S., Sabate R., Saupe S.J., and Steven A.C.
Mass analysis by scanning transmission electron microscopy and electron diffraction validate predictions of the stacked β-solenoid model of HET-s prion fibrils.
J of Bio Chem ., 282(8):5545-50 (2007).  PubMed
Spano A.J., Chen F.S., Goodman B.E., Sabat A.E., Simon M.N., Wall J.S., Correia J.J., McIvor W., Newcomb W.W., Brown J.C., Schnur J.M., and Lebedev N.
In vitro assembly of a prohead-like structure of the Rhodobacter capsulatus gene transfer agent.
Virology, 364(1):95-102 (2007).  PubMed
Diaz-Avalos R., King C.Y., Wall J.S., Simon M.N., and Caspar D.L.
Strain-specific morphologies of yeast prion amyloid fibrils.
Proc Natl Acad Sci USA, 102(29):10165-70 (2005).   PubMed   Full Text
Ksiezak-Reding H., and Wall J.S.
Characterization of paired helical filaments by scanning transmission electron microscopy.
Micros Res Tech., 67(3-4):126-140 (2005).   PubMed
Lashuel H.A., and Wall J.S.
Molecular electron microscopy approaches to elucidating the mechanisms of protein fibrillogenesis.
in: Methods in Molecular Biology Vol. 299: Amyloid Proteins: Methods and Protocols, E. M. Sigurdsson, Editor, Chapter 7, pp. 81-102, Humana Press, Inc., Totowa, NJ (2005).   PubMed
Ding S.-Y., Rumbles G., Jones M., Tucker M.P., Nedeljkovic J., Simon M.N., Wall J.S., Himmel M.E.
Bioconjugation of (CdSe)ZnS quantum dots using a genetically engineered multiple polyhistidine tagged cohesin/dockerin protein polymer.
Macromol Materials Eng. 289:622-628 (2004).
Mosesson M.W., DiOrio J.P., Hernandez I., Hainfeld J.F., Wall J.S., and Grieninger G.
The ultrastructure of fibrinogen-420 and the fibrin-420 clot.
Biophys Chem., 112: 209-214 (2004).   PubMed
Baxa U., Taylor K.L., Wall J.S., Simon M.N., Cheng N., Wickner R.B., and Steven A.C.
Architecture of Ure2p prion filaments: The N-terminal domains form a central core fiber.
J Biol Chem., 278(44):43717-27 (2003).   PubMed   Full Text
Chen W., De Geronimo G., Li Z., O'Connor P., Radeka V., Rehak P., Smith G.C., Wall J.S., Yu B.
High resistivity silicon active pixel sensors for recording data
from STEM Nuclear Instruments & Methods in Physics research section A-accelerators Spectrometers Detectors and Associated equipment, 512:368-377 (2003).
Ding S.-Y., Jones M., Tucker M.P., Nedeljkovic J.M., Wall J., Simon M.N., Rumbles G., and Himmel M.E.
Quantum Dot Molecules Assembled with Genetically Engineered Proteins.
NanoLetters, 3:1581-1585 (2003).
Ding S.-Y., Rumbles G., Jones M., Tucker M.P., Nedeljkovic J., Wall J., and Himmel M.E.
Quantum Dots Stabilized By Genetically Engineered Proteins.
Mat Res Soc Symp Proc., 774:165-170 (2003).
Lashuel H.A., Hartley D.M., Petre B.M., Wall J.S., Simon M.N., Walz T., and Lansbury P.T.
Mixtures of wild-type and a pathogenic (E22G) form of Abeta40 in vitro accumulate protofibrils, including amyloid pores.
J Mol Biol., 332:795-808 (2003).   PubMed
Mosesson M.W., Siebenlist K.R., Hernandez I., Wall J.S. and Hainfeld J.F.
Fibrinogen assembly and crosslinking on a fibrin fragment E template.
Thromb Haemost., 87:651-8 (2002).   PubMed
Watts N.R., Jones L.N., Cheng N., Wall J.S., Parry D.A. and Steven A.C.
Cryo-electron microscopy of trichocyte (hard alpha-keratin) intermediate filaments reveals a low-density core.
J Struct Biol., 137:109-18 (2002).   PubMed
King M.E., Ghoshal N., Wall J.S., Binder L.I., and Ksiezak-Reding H.
Structural analysis of Pick's disease-derived and in vitro-assembled tau filaments.
Am J Pathol., 158:1481-90 (2001).   PubMed   Full Text
Parker S.D., Wall J.S. and HunterF.
Analysis of Mason-Pfizer monkey virus Gag particles by scanning transmission electron microscopy.
J Virol., 75: 9543-8 (2001).   PubMed   Full Text
Wall J.S. and Simon M.N.
Scanning transmission electron microscopy of DNA-protein complexes.
Methods Mol Biol., 148:589-601 (2001).   PubMed
Kuchumov A.R., Taveau J.C., Lamy J.N., Wall J.S., Weber R.E., and Vinogradov S.N.
The role of linkers in the reassembly of the 3.6 MDa hexagonal bilayer hemoglobin from Lumbricus terrestris.
J Mol Biol., 289:1361-1374 (1999).   PubMed
Wall J.S.
Visualizing "greengold" clusters in the STEM.
J Struct Biol., 27:161-168 (1999).   PubMed
Ksiezak-Reding H., Yang G., Simon M., and Wall J.S.
Assembled tau filaments differ from native paired helical filaments as determined by scanning transmission electron microscopy (STEM).
Brain Res., 814:86-98 (1998).   PubMed
Mosesson M.W., Siebenlist K.R., Meh D.A., Wall J.S. and Hainfeld J.F.
The location of the carboxy-terminal region of gamma chains in fibrinogen and fibrin D domains.
Proc Natl Acad Sci USA, 95:10511-10516 (1998).   PubMed   Full Text
Wall J.S., Hainfeld J.F. and Simon M.N.
Scanning transmission electron microscopy of nuclear structures.
In: Nuclear Strcture and Function, M. Berrios (ed.) Methods in Cell Biology, vol. 53, San Diego: Academic Press, pp 139-164 (1998).   PubMed
Thomas D., Schultz P., Steven A.C., and Wall J.S.
Mass analysis of biological macromolecular complexes by STEM.
Biol Cell, 80:181-192 (1994).   PubMed
Tumminia S.J., Hellmann W., Wall J.S. and Boublik M.
Visualization of the protein-nucleic acid interactions involved in the in vitro assembly of the Escherichia coli 50S ribosomal subunit.
J Mol Biol., 235:1239-1250 (1994).   PubMed
Mosesson M.W., Di Orio J.P., Siebenlist K.R., Wall J.S. and Hainfeld J.F.
Evidence for a second type of fibril branch point in fibrin polymer networks,the trimolecular junction.
Blood, 82:1517-1521 (1993).   PubMed
Wall J.S. and Hainfeld J.F.
Mass mapping with the Scanning Transmission Electron Microscope.
Ann Rev Biophys Chem., 15:355-376 (1986).   PubMed
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