Mavoungou D, Mavoungou E, Macka G; International Conference on AIDS.
Int Conf AIDS. 2000 Jul 9-14; 13: abstract no. MoPeA2003.
D. Mavoungou, PO Box 12134, Libreville, Gabon, Tel.: +241 731 805, Fax: +241 731 804, E-mail: crph2000@yahoo.fr
Background: Trimolecular complexe gp 120-CD4-coreceptor inducing phospholipase A2 (PLA2) activation through proteinkinase C (PKC) may have a critical role for fusion between membrane phospholipids of cellular host and gp41 for HIV1 entry in cells. We present here the capacity to inhibit HIV1 envelope glycoproteins mediated-cell fusion of IM28 a new immunotherapeutic drug (D. Mavoungou, INPI, 9904706, France). Methods: T-cell line expressing stably HIV-1 envelope glycoprotein was co-cultured with an equal number of T cell lines expressing CD4 in absence or presence of IM28. Inhibition of cell-cell fusion induced by IM28 was also compared DHEA and to others compounds which are known to interact with HIV envelope proteins such as dextran sulfate, suramin, heparin, dexamethasone, concavalin A, provalbumin and troponin. Results: TF228.1.16 which expressed HIV-1 envelope glycoproteins fuses with 293/CD4+, a T cell expressing CD4 inducing numerous large syncytium in absence of corticoids. A concentration of IM28 ranging from 15 to 45 m g/ml was able to inhibit completely that fusion; during infection of Sup T1 cells with TF228.1.16, syncytium appeared more rapidly than those observed in 293/CD4+ cell line. Except provalbumin and troponin, IM28 and other compounds mentioned above inhibit syncytia induced by Sup T1; dexamethasone, DHEA and IM28 did not lyse the syncytia already formed but stopped the fusion reaction and apparition of new syncytia. IM28 presented less toxicity for cells than DHEA. Conclusions: IM28, a C19 steroid derivative seems to have an anti HIV1 activity by inhibiting HIV envelope glycoproteins-mediated cell fusion.
Publication Types:
Keywords:
- Antigens, CD4
- CD4-Positive T-Lymphocytes
- Cell Communication
- Cell Fusion
- Cell Line
- France
- Giant Cells
- Glycoproteins
- HIV-1
- Membrane Fusion
- T-Lymphocytes
- immunology
Other ID:
UI: 102237553
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