myo-Inositol Metabolism in Lilium longiflorum Pollen: Uptake and Incorporation of myo-Inositol-2-3H 1

Plant Physiol. 1977 April; 59(4): 653–657.

PMCID: PMC542467

myo-Inositol Metabolism in Lilium longiflorum Pollen

Uptake and Incorporation of myo-Inositol-2-³H ¹

Minshen Chen² and Frank A. Loewus³

^aDepartment of Agricultural Chemistry, Washington State University, Pullman, Washington 99164

² Present address: Department of Biochemistry, Purdue University, West Lafayette, Ind. 47907.

³ To whom inquiries and requests for reprints should be addressed.

¹ Scientific Paper No. 4689, Project 0266, College of Agriculture Research Center, Washington State University, Pullman, Wash. 99164. This work was supported in part by Grants GM-12422 and GM-22427 from the National Institute of General Medical Sciences, National Institutes of Health.

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Abstract

Germinating Lilium longiflorum pollen absorbs and metabolizes myo-inositol-2-³H (MI-2-³H) with a pronounced lag when label is supplied from the beginning of germination. If MI-2-³H is given after 3 hours of germination, incorporation of labeled metabolic products into pollen tube polysaccharides is constant over a range of 0.56 mm to 2.78 mm MI. When MI-2-³H is supplied as a 0.5-hour pulse 3 hours after germination, the proper precursor-product relationship to tube wall polysaccharides is observed. Replacing 10% of the germination media with sigmatic exudate from a compatible cultivar hastens germination and tube elongation. Enhanced MI metabolism accompanies tube growth in this exudate-enriched media.

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