MCELHANEY JE, HERRE JM, COLE SK, HOOTON JW; Interscience Conference on Antimicrobial Agents and Chemotherapy (42nd : 2002 : San Diego, Calif.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2002 Sep 27-30; 42: abstract no. G-1218.
Eastern Virginia Medical School, Norfolk, VA.
BACKGROUND: Congestive heart failure (CHF) adds to the increased risk of influenza illness in older adults but exacerbations of CHF during the influenza season are prevented by vaccination. The effect of CHF on the immune response to vaccination is unknown. The hypothesis was that CHF would diminish the T-cell response to influenza vaccination. METHODS: We studied the effect of CHF and performance on the 6-minute walk test (6-MWT) on T-lymphocyte responses to influenza vaccination in three groups of older adults; healthy, Class II CHF, Class III/IV CHF. Cytokine and granzyme B (GrzB) levels were measured in ex vivo virus-activated peripheral blood mononuclear cell cultures prior to and 4 and 12 weeks after administration of the 2000-01 trivalent influenza vaccine. RESULTS: All groups had a significant T helper type 1 response to vaccination with an increase in interferon-gamma (IFN-g) levels, a decline in interleukin-10 (IL-10) levels (p<.0001) and increase in cytotoxic T lymphocyte (CTL) activity measured by GrzB (p<.0001). The 6-MWT was significant variable in the analysis of cytokines and Grz B. After adjustment for the 6-MWT, the Class III/IV CHF group had a lower ratio of IFN-g:IL-10 levels compared to the Class II and healthy groups (p<.01) following vaccination. The IFN-g:IL-10 ratios were higher in PBMC stimulated with the H1N1 vaccine strain compared to the H3N2 and B strains of influenza. Both CHF groups had higher GrzB levels compared to healthy subjects before and after vaccination (p<.0001). The predicted negative correlation between Grz B and IL-10 levels (r=0.3, p<.0001) explained some of the difference in Grz B between healthy and CHF groups. CONCLUSIONS: Current killed virus vaccines effectively stimulate a Th1 response in older people including those with CHF. As predicted, these vaccines are poor stimulators of the CTL memory but the cytokine milieu related to CHF may augment the CTL response to influenza infection.
Publication Types:
Keywords:
- Adult
- Cytokines
- Granzymes
- Heart Failure
- Humans
- Influenza Vaccines
- Influenza, Human
- Interferon Type II
- Interleukin-10
- Serine Endopeptidases
- T-Lymphocytes
- T-Lymphocytes, Cytotoxic
- Vaccination
Other ID:
UI: 102268367
From Meeting Abstracts