MCELHANEY JE, GRAVENSTEIN S, HOOTON JW, ARORA A; Interscience Conference on Antimicrobial Agents and Chemotherapy (42nd : 2002 : San Diego, Calif.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2002 Sep 27-30; 42: abstract no. G-145.
Eastern Virginia Medical School, Norfolk, VA.
BACKGROUND: Influenza vaccination may not provide protection through the influenza season in older adults. The effect of a two-dose vaccination regimen given 16 weeks apart was compared to a single-dose vaccination. The hypothesis was that booster vaccine recipients would have less T-cell activation compared to single-dose recipients 4 weeks post-vaccination. METHODS: The immune response to standard trivalent split-virus influenza vaccine was compared in two groups of healthy older adults. One group received the standard dose (SD) of influenza vaccine. The booster dose (BD) group was re-vaccinated 16 weeks after an initial dose of vaccine. Serum antibody responses and ex vivo T-cell responses in influenza virus-activated peripheral blood mononuclear cells were measured. RESULTS: 4-week post-vaccination antibody titers were similar for the two groups. Interleukin-10 (IL-10) levels were higher pre-vaccination in the SD versus the BD group (p<.0001). IL-10 decreased in the SD but did change in the BD group such that IL-10 levels were similar in the two groups after vaccination. The ratio of interferon-gamma (IFN-g) to IL-10 levels increased in the SD group but declined in the BD group following vaccination. Granzyme B (Grz B) levels reflecting cytotoxic T-lymphocyte activity were similar pre-vaccination but were higher in the SD compared to the BD following vaccination (p=.01). There were strain-related differences in the cytokine but not the Grz B response to vaccination. CONCLUSIONS: These results suggest that vaccination stimulates a T helper type 1 (Th1) response that suppresses IL-10 levels for at least 16 weeks following vaccination. Booster vaccination suppresses the Th1 response with a reduction in the IFN-g:IL-10 ratio and is associated with lower levels of Grz B compared to a standard dose regimen. Post-vaccination antibody titers were not affected by booster vaccination.
Publication Types:
Keywords:
- Adult
- Aged
- Antibody Formation
- Cytokines
- Granzymes
- Humans
- Immunization, Secondary
- Influenza Vaccines
- Interferon Type II
- Interleukin-10
- Orthomyxoviridae
- Serine Endopeptidases
- T-Lymphocytes
- T-Lymphocytes, Cytotoxic
- Vaccination
- immunology
Other ID:
UI: 102268363
From Meeting Abstracts