Kakimoto WM, Gettie A, Segal JP, Marx P, Smith S, Connor R, Nixon DF; Keystone Symposia on Molecular and Cellular Biology: 1998 HIV Pathogenesis and Treatment.
Keyst Symp Mol Cell Biol Keyst Symp Mol Cell Biol. 1998 Mar 13-19; 92 (abstract no. 4047).
Aaron Diamond AIDS Research Center, New York, NY.
The detection of SIV specific CTL is critical in understanding the primate immune response to SIV infection and for the development of HIV vaccines. We compared several methods of SIV CTL restimulation in vitro both antigen specific, (with autologous B-lymphoblastoid cells (BCL) infected with recombinant vaccinia virus expressing SIV gene products fixed with paraformaldehyde), and polyclonal, with concanavalin A (ConA) and Staphylococcal enterotoxin B (SEB). Both freshly isolated and cryopreserved PBMC from SIV infected animals were cultured for 10-14 days, in bulk culture and at limiting dilution, with recombinant IL-2 added after the third day of stimulation. At day 14, cultures were assayed in a standard chromium release assay on autologous BCL targets infected with a panel of recombinant vaccinia viruses expressing SIV gene products. CTL responses were detected with all three methods of restimulation, with the BCL rVV infected, paraformaldehyde fixed stimulated CTL eliciting the strongest responses. However, polyclonal stimulation may be advantagous where cell numbers are limited or antigenic specificities are unknown.
Publication Types:
Keywords:
- AIDS Vaccines
- Epitopes
- In Vitro
- Simian immunodeficiency virus
- T-Lymphocytes, Cytotoxic
- Vaccinia virus
- immunology
- methods
Other ID:
UI: 102236898
From Meeting Abstracts