Moller BK; IAS Conference on HIV Pathogenesis and Treatment (2nd : 2003 : Paris, France).
Antivir Ther. 2003; 8 (Suppl.1): abstract no. 459.
Dept. of Clinical Immunology, Aarhus University Hospital, Denmark
Dendritic cells (DC) have recently been considered an important reservoir for the initial spreading of HIV to regional lymph nodes following infection and subsequently to CD4+ T cells encountering infected DC. We investigated the phenotypical changes of circulating dendritic cells of HIV-infected patients by flow cytometry. Dendritic cells were defined as CD4+ mononuclear cells lacking CD3 and CD14 expression, and the expression of CD11c, CD86, HLA-DR and CXCR4 was measured after logarithmic amplification of 4-colour fluorescence signals and software compensation for spectral overlap of listmode data. CD86 expression was confined to CD11c+ DC in cord blood, peripheral blood from healthy adults as well as adult HIV-infected patients. CD11c is generally considered a marker of myeloid DC. CD86 expression was unaltered or slightly upregulated, whereas HLA-DR expression was two- to eightfold upregulated in DC from HIV patients, similar to the increase in HLA-DR expression of CD4- T cells as compared to cells from healthy adults. CXCR4 expression was at or below detection limit in peripheral blood DC in cord blood and peripheral blood from controls or HIV patients, whereas CXCR4 expression in CD4+ T cells was significantly upreg-ulated in HIV patients, and exhibiting positive correlation to HLA-DR expression of CD4- T cells. In conclusion, phenotypical changes in DC of HIV patients are modest, but activation as deducted from increased expression of HLA-DR is not reflected in modulations of CD86 or CXCR4 expression.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Adult
- Anti-HIV Agents
- Antigens, CD
- Antigens, CD11c
- Antigens, CD4
- CD86 protein, human
- Dendritic Cells
- Flow Cytometry
- HIV
- HIV Antibodies
- HIV Infections
- HIV Seronegativity
- HIV Seropositivity
- HLA-DR Antigens
- Humans
- Membrane Glycoproteins
- Receptors, CXCR4
- T-Lymphocytes
- immunology
Other ID:
UI: 102262743
From Meeting Abstracts