Energy Citations Database

Bibliographic Citation

 
Document
For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field.
For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability.
DOI 10.1016/j.taap.2006.07.008
Title Regional specificity in deltamethrin induced cytochrome P450 expression in rat brain
Creator/Author Yadav, Sanjay [Developmental Toxicology Division, Industrial Toxicology Research Centre, PO Box 80, Mahatma Gandhi Marg, Lucknow-226 001, U.P. (India)] ; Johri, Ashu [Developmental Toxicology Division, Industrial Toxicology Research Centre, PO Box 80, Mahatma Gandhi Marg, Lucknow-226 001, U.P. (India)] ; Dhawan, Alok [Developmental Toxicology Division, Industrial Toxicology Research Centre, PO Box 80, Mahatma Gandhi Marg, Lucknow-226 001, U.P. (India)] ; Seth, Prahlad K. [Developmental Toxicology Division, Industrial Toxicology Research Centre, PO Box 80, Mahatma Gandhi Marg, Lucknow-226 001, U.P. (India)] ; Parmar, Devendra [Developmental Toxicology Division, Industrial Toxicology Research Centre, PO Box 80, Mahatma Gandhi Marg, Lucknow-226 001, U.P. (India)]. E-mail: parmar_devendra@hotmail.com
Publication Date2006 Nov 15
OSTI IdentifierOSTI ID: 20850474
Other Number(s)Journal ID: ISSN 0041-008X; TXAPA9; TRN: US07R1736020936
Resource TypeJournal Article
Resource RelationJournal: Toxicology and Applied Pharmacology; Journal Volume: 217; Journal Issue: 1; Other Information: DOI: 10.1016/j.taap.2006.07.008; PII: S0041-008X(06)00259-6; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Subject60 APPLIED LIFE SCIENCES; ANTIBODIES; CEREBELLUM; HIPPOCAMPUS; HYPOTHALAMUS; ISOENZYMES; LIVER; ORAL ADMINISTRATION; POLYMERASE CHAIN REACTION; RATS; SPECIFICITY
Description/Abstract Oral administration of deltamethrin (5 mg/kg x 7 or 15 or 21 days) was found to produce a time-dependent increase in the mRNA expression of xenobiotic metabolizing cytochrome P450 1A1 (CYP1A1), 1A2 and CYP2B1, 2B2 isoenzymes in rat brain. RT-PCR studies further showed that increase in the mRNA expression of these CYP isoenzymes observed after 21 days of exposure was region specific. Hippocampus exhibited maximum increase in the mRNA expression of CYP1A1, which was followed by pons-medulla, cerebellum and hypothalamus. The mRNA expression of CYP2B1 also exhibited maximum increase in the hypothalamus and hippocampus followed by almost similar increase in midbrain and cerebellum. In contrast, mRNA expression of CYP1A2 and CYP2B2, the constitutive isoenzymes exhibited relatively higher increase in pons-medulla, cerebellum and frontal cortex. Immunoblotting studies carried out with polyclonal antibody raised against rat liver CYP1A1/1A2 or CYP2B1/2B2 isoenzymes also showed increase in immunoreactivity comigrating with CYP1A1/1A2 or 2B1/2B2 in the microsomal fractions isolated from hippocampus, hypothalamus and cerebellum of rat treated with deltamethrin. Though the exact relationship of the xenobiotic metabolizing CYPs with the physiological function of the brain is yet to be clearly understood, the increase in the mRNA expression of the CYPs in the brain regions that regulate specific brain functions affected by deltamethrin have further indicated that modulation of these CYPs could be associated with the various endogenous functions of the brain.
Country of PublicationUnited States
LanguageEnglish
FormatSize: page(s) 15-24
System Entry Date2007 Apr 02

Top