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Title Visceral organ mass and hepatic protein synthetic capacity in fed and fasted rats
Creator/Author Burrin, D.G. ; Britton, R.A. ; Ferrell, C.L.
Publication Date1986 Mar 01
OSTI IdentifierOSTI ID: 5393752
Report Number(s)CONF-8604222-
Other Number(s)CODEN: FEPRA
Resource TypeConference
Specific TypeJournal Article
Resource RelationFed. Proc., Fed. Am. Soc. Exp. Biol. ; Vol/Issue: 45:3; 70. annual meeting of the Federation of American Society for Experimental Biology; 13 Apr 1986; St. Louis, MO, USA
Research OrgUniv. of Nebraska, Lincoln
Subject550201 -- Biochemistry-- Tracer Techniques; FASTING-- BIOLOGICAL EFFECTS;PROTEINS-- BIOSYNTHESIS; CARBON 14 COMPOUNDS;DNA;INTESTINES;KIDNEYS;LIVER;LIVER CELLS;OXYGEN;PERFUSED TISSUES;RATS;STOMACH;TRACER TECHNIQUES;WEIGHT
Related SubjectANIMAL CELLS;ANIMAL TISSUES;ANIMALS;BODY;DIGESTIVE SYSTEM;ELEMENTS;GASTROINTESTINAL TRACT;GLANDS;ISOTOPE APPLICATIONS;LABELLED COMPOUNDS;MAMMALS;NONMETALS;NUCLEIC ACIDS;ORGANIC COMPOUNDS;ORGANS;RODENTS;SOMATIC CELLS;SYNTHESIS;TISSUES;VERTEBRATES
Description/Abstract Forty-two male rats (avg wt.^= 320 g) were used to assess the effect of severe nutrient restriction (72 h fast) on visceral organ mass and hepatic protein synthetic capacity as measured by in vitro incorporation of U-/sup 14/-C-VALINE (/sup 14/C-VAL) into isolated hepatocytes.^Organ weights expressed as a percent of empty body weight for fed vs. fasted rats were; liver (5.21 +/- .54 vs 3.82 +/- .46), kidney (.87 +/- 0.6 vs .89 +/- .05), stomach (.60 +/- .06 vs .61 +/- .06), intestines (3.70 +/- .44 vs 3.41 +/- .37).^No differences were observed in in vitro oxygen consumption (15.7 +/- 3.1 vs 16.1 +/- 3.3, umole min/sup -1/ g/sup -1/ dry tissue) or /sup 14/-C VAL incorporation (4.93 +/- 1.28 vs 4.31 +/- 1.48, dpm min/sup -1/ mg/sup -1/ dry tissue) for hepatocytes from fed vs. fasted rats.^Analysis of perfused liver tissue indicated fed rats had higher protein (152.1 +/- 16.3 vs 136.6 +/- 29.6, mg/g tissue) and RNA (8.81 +/- 1.66 vs 5.97 +/- 1.87, mg/g tissue) with lower DNA (2.19 +/- .31 vs 3.19 +/- .54, mg/g tissue) compared to fasted rats.^Protein-nucleic acid ratios suggest liver tissue from fed rats had a greater capacity for protein synthesis compared to fasted rats, however, this was not evident from in vitro hepatocyte /sup 14/-C VAL incorporation estimates.^These data indicate that severe nutrient restriction (72 h fast) affects visceral organ mass largely by reduced liver and gut size as well as decreased hepatic protein synthetic capacity.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 233
System Entry Date2001 May 13

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