Bibliographic Citation
Document | For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field. For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability. |
---|---|
Title | Visceral organ mass and hepatic protein synthetic capacity in fed and fasted rats |
Creator/Author | Burrin, D.G. ; Britton, R.A. ; Ferrell, C.L. |
Publication Date | 1986 Mar 01 |
OSTI Identifier | OSTI ID: 5393752 |
Report Number(s) | CONF-8604222- |
Other Number(s) | CODEN: FEPRA |
Resource Type | Conference |
Specific Type | Journal Article |
Resource Relation | Fed. Proc., Fed. Am. Soc. Exp. Biol. ; Vol/Issue: 45:3; 70. annual meeting of the Federation of American Society for Experimental Biology; 13 Apr 1986; St. Louis, MO, USA |
Research Org | Univ. of Nebraska, Lincoln |
Subject | 550201 -- Biochemistry-- Tracer Techniques; FASTING-- BIOLOGICAL EFFECTS;PROTEINS-- BIOSYNTHESIS; CARBON 14 COMPOUNDS;DNA;INTESTINES;KIDNEYS;LIVER;LIVER CELLS;OXYGEN;PERFUSED TISSUES;RATS;STOMACH;TRACER TECHNIQUES;WEIGHT |
Related Subject | ANIMAL CELLS;ANIMAL TISSUES;ANIMALS;BODY;DIGESTIVE SYSTEM;ELEMENTS;GASTROINTESTINAL TRACT;GLANDS;ISOTOPE APPLICATIONS;LABELLED COMPOUNDS;MAMMALS;NONMETALS;NUCLEIC ACIDS;ORGANIC COMPOUNDS;ORGANS;RODENTS;SOMATIC CELLS;SYNTHESIS;TISSUES;VERTEBRATES |
Description/Abstract | Forty-two male rats (avg wt.^= 320 g) were used to assess the effect of severe nutrient restriction (72 h fast) on visceral organ mass and hepatic protein synthetic capacity as measured by in vitro incorporation of U-/sup 14/-C-VALINE (/sup 14/C-VAL) into isolated hepatocytes.^Organ weights expressed as a percent of empty body weight for fed vs. fasted rats were; liver (5.21 +/- .54 vs 3.82 +/- .46), kidney (.87 +/- 0.6 vs .89 +/- .05), stomach (.60 +/- .06 vs .61 +/- .06), intestines (3.70 +/- .44 vs 3.41 +/- .37).^No differences were observed in in vitro oxygen consumption (15.7 +/- 3.1 vs 16.1 +/- 3.3, umole min/sup -1/ g/sup -1/ dry tissue) or /sup 14/-C VAL incorporation (4.93 +/- 1.28 vs 4.31 +/- 1.48, dpm min/sup -1/ mg/sup -1/ dry tissue) for hepatocytes from fed vs. fasted rats.^Analysis of perfused liver tissue indicated fed rats had higher protein (152.1 +/- 16.3 vs 136.6 +/- 29.6, mg/g tissue) and RNA (8.81 +/- 1.66 vs 5.97 +/- 1.87, mg/g tissue) with lower DNA (2.19 +/- .31 vs 3.19 +/- .54, mg/g tissue) compared to fasted rats.^Protein-nucleic acid ratios suggest liver tissue from fed rats had a greater capacity for protein synthesis compared to fasted rats, however, this was not evident from in vitro hepatocyte /sup 14/-C VAL incorporation estimates.^These data indicate that severe nutrient restriction (72 h fast) affects visceral organ mass largely by reduced liver and gut size as well as decreased hepatic protein synthetic capacity. |
Country of Publication | United States |
Language | English |
Format | Pages: 233 |
System Entry Date | 2001 May 13 |
Top |